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General Information
Symbol
Dmel\wun49
Species
D. melanogaster
Name
FlyBase ID
FBal0264542
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C9402702T

Reported nucleotide change:

C?T

Amino acid change:

P189L | wun-PA; P268L | wun-PB; P268L | wun-PC

Reported amino acid change:

P268L

Comment:

Reported as P268H but corrected to P268L.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: P268L.

Revision of amino acid replacement data reported in FBrf0210764.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Other
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference

79% of embryos lacking both maternal and zygotic wun and wun2 function (derived from females carrying homozygous double mutant wun49 wun2EP2650ex34 germline clones mated to wun wun2 double mutant males) have aberrant tracheal morphology. Breaks in the dorsal trunk plus bulbous chitin accumulation throughout the dorsal and lateral trunk and transverse connectives are seen.

Very few animals which lack both maternal and zygotic wun and wun2 function (derived from females containing double mutant wun49 wun2EP2650ex34 germline clones mated to Df(2R)wun-GL/+ or Ab(2;?)wunCE/+ males) survive to adulthood. This lethality is only partially rescued by expression of either wunScer\UAS.T:Avic\GFP-EGFP or wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4btl.PS.

Embryos derived from females carrying homozygous wun49 wun2EP2650ex34 germline clones crossed to wild type males are lethal in late embryogenesis. These embryos form a wild type number of germ cells that are correctly positioned in the midgut pocket at stage nine. wun49 wun2EP2650ex34 mutant germ cells fail to individualise, but are still able to cross the midgut as a cluster, although this occurs at stage 11 rather than stage 10. However they fail to migrate further into the overlying mesoderm and eventually undergo cell death. Cell death is first observed at stage 10 and by stage 15/16 the number of germ cells is reduced to on average 1.4 (compared to approximately 21 in wild type). The remaining wun49 wun2EP2650ex34 mutant germ cells also exhibit defects in distribution to the gonads. When either 2 or 4 germ cells are present the germ cells distribute randomly between the two gonads, whereas when 3 germ cells are present there is a significant bias towards all germ cells migrating to the same gonad.

Expression of wunScer\UAS.cZa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the germ cell death in embryos derived from females carrying homozygous wun49 wun2EP2650ex34 germline clones and wild type males.

Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the germ cell death in embryos derived from females carrying homozygous wun49 wun2EP2650ex34 germline clones and wild type males.

Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is unable to rescue the germ cell death seen in embryos derived from females carrying homozygous wun49 wun2EP2650ex34 germline clones and wild type males.

Embryos derived from females carrying wun49 wun2EP2650ex34 germline clones and Df(2R)wun-GL males have a less severe germ cell death phenotype than embryos produced when females with wun49 wun2EP2650ex34 germline clones are crossed to wild type males (10.6 germ cells on average compared to 1.4). The surviving germ cells are found exclusively within the midgut and fail to individualise at stage 10. Most germ cells do not cross the midgut epithelium, instead remaining in a clump inside the midgut pocket. Occasional germ cells detach from the clump and migrate within the midgut with normal amoeboid-like motion.

Loss of maternal shgA9-49 is unable to rescue the germ cell migration defects seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones crossed to Df(2R)wun-GL males. Germ cell dispersal can be seen at stage 10.

Expression of Rho1N19.Scer\UAS in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones. No germ cells can be detected outside of the gut.

Expression of Rac1V12.Scer\UAS in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones. No germ cells can be detected outside of the gut.

Expression of Rac1N17.Scer\UAS in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones.

Expression of Rho1V14.Scer\UAS in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones.

Expression of Rho1Scer\UAS.cMa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones.

Homozygous srp2 rescues the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones. The surviving germ cells are found in a tight clump inside the gut but also frequently in loosely associated groups outside of the gut. These germ cells are of normal size and morphology. Heterozygous srp2 is unable to rescue the phenotype.

Wild-type stage 5 germ cells transplanted into the posterior pole of stage 5 wun49 wun2EP2650ex34 host embryos does not prevent germ cell death from occuring at stage 14. No correlation can be seen between cell transplanted and the number of surviving germ cells.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)