FlyBase Allele Report: Dmel\MAPk-Ak2[Δ43]

General Information

Symbol

Dmel\MAPk-Ak2^Δ43

Species

D. melanogaster

Name

FlyBase ID

FBal0267165

Feature type

allele

Associated gene

Dmel\MAPk-Ak2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Seisenbacher et al., 2011)

Mutagen

P-element activity

(Seisenbacher et al., 2011)

Progenitor genotype

P{EP}MAPk-Ak2^GE3296

(Seisenbacher et al., 2011)

Cytology

Description

Excision of the P{EP} element in P{EP}MAPk-Ak2^GE3296 results in deletion of the region downstream of the insertion site, which includes the second exon and most of the third exon of MAPk-Ak2.

(Seisenbacher et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

melanotic mass phenotype | larval stage | nutrition conditional

(Seisenbacher et al., 2011)

partially lethal - majority live | nutrition conditional

(Seisenbacher et al., 2011)

viable

(Seisenbacher et al., 2011)

Phenotype Manifest In

embryonic/larval hindgut | larval stage | posterior | nutrition conditional

(Seisenbacher et al., 2011)

Detailed Description

Statement

Reference

No obvious phenotypes are seen in MAPk-Ak2^Δ43 homozygous mutants under normal conditions.

Approximately 38% of MAPk-Ak2^Δ43 mutant larvae exhibit a melanotic phenotype in response to strong salt stress (0.2M NaCl). A "black dot" (BD) is seen in the posterior hindgut and the affected hindgut epithelium appears pathologically altered. Approximately 20% of mutants exhibit this phenotype when raised on food containing 0.1M NaCl and no phenotypes are observed when MAPk-Ak2^Δ43 mutants are raised on normal food. The dorsal epithelial cells (ECs) containing BDs are apically ruptured, whereas no damage is detected in wild type hindguts under the same conditions. No morphological alterations are seen in MAPk-Ak2^Δ43 mutant ECs that do not contain a BD, although a blistering of the surface without damage to the apical membrane is occasionally observed. TUNEL staining reveals that local clusters of apoptotic hindgut ECs are present in mutant larvae under salt stress conditions, and these are not present in controls. The visceral muscles surrounding the hindgut ECs appear unaffected, with a disturbed hindgut musculature only observed in hindguts with large BDs. Loss of epithelial integrity close to BDs is also observed, as determined by more diffuse Avic\GFP^Nrg-G00305 expression. BDs are only seen in the dorsal hindgut hd compartment.

(Seisenbacher et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, suppressible | partially by Mkk4^G587/Mkk4^G414

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, suppressible | partially by Scer\GAL4^en-e16E/bsk^K53R.UAS

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, suppressible | partially by Scer\GAL4^puc-GAL4E69/bsk^K53R.UAS

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, suppressible by Scer\GAL4^en-e16E/Mmus\Mapkapk2^UAS.cSa

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, suppressible | partially by Tak1²

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, suppressible | partially by Tak1⁴

(Seisenbacher et al., 2011)

NOT suppressed by

Statement

Reference

MAPk-Ak2^Δ43 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, non-suppressible by Mmus\Bax^UAS.cGa/Scer\GAL4^He.PZ

(Seisenbacher et al., 2011)

Enhancer of

Statement

Reference

MAPk-Ak2^Δ43 is an enhancer of partially lethal - majority die | nutrition conditional phenotype of p38a¹

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is an enhancer of melanotic mass phenotype | larval stage phenotype of p38b^d27

(Seisenbacher et al., 2011)

NOT Enhancer of

Statement

Reference

MAPk-Ak2^Δ43 is a non-enhancer of melanotic mass phenotype | larval stage | nutrition conditional phenotype of p38b^d27

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is a non-enhancer of partially lethal - majority die phenotype of p38b^d27

(Seisenbacher et al., 2011)

Other

Statement

Reference

MAPk-Ak2^Δ43, p38a¹ has melanotic mass phenotype | larval stage | nutrition conditional phenotype

(Seisenbacher et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, suppressible | partially by Tak1²

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, suppressible | partially by Tak1⁴

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, suppressible | partially by Mkk4^G587/Mkk4^G414

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, suppressible | partially by Scer\GAL4^en-e16E/bsk^K53R.UAS

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, suppressible | partially by Scer\GAL4^puc-GAL4E69/bsk^K53R.UAS

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, suppressible by Scer\GAL4^en-e16E/Mmus\Mapkapk2^UAS.cSa

(Seisenbacher et al., 2011)

NOT suppressed by

Statement

Reference

MAPk-Ak2^Δ43 has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype, non-suppressible by Mmus\Bax^UAS.cGa/Scer\GAL4^He.PZ

(Seisenbacher et al., 2011)

Enhancer of

Statement

Reference

MAPk-Ak2^Δ43 is an enhancer of embryonic/larval hindgut | larval stage | posterior phenotype of p38b^d27

(Seisenbacher et al., 2011)

NOT Enhancer of

Statement

Reference

MAPk-Ak2^Δ43 is a non-enhancer of embryonic/larval hindgut | larval stage | posterior | nutrition conditional phenotype of p38b^d27

(Seisenbacher et al., 2011)

Other

Statement

Reference

MAPk-Ak2^Δ43, p38a¹ has embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype

(Seisenbacher et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Tak1² partially suppresses the "black dot" phenotype seen in the hindgut of MAPk-Ak2^Δ43 larvae raised on either 0.1M or 0.2M NaCl.

Tak1⁴ partially suppresses the "black dot" phenotype seen in the hindgut of MAPk-Ak2^Δ43 larvae raised on either 0.1M or 0.2M NaCl.

Mkk4^G587/Mkk4^G414 partially suppresses the "black dot" phenotype seen in the hindgut of MAPk-Ak2^Δ43 larvae raised on either 0.1M or 0.2M NaCl.

Expression of bsk^{K53R.Scer\UAS} under the control of either Scer\GAL4^en-e16E or Scer\GAL4^puc-GAL4E69 partially suppresses the "black dot" phenotype seen in MAPk-Ak2^Δ43 larvae.

MAPk-Ak2^Δ43 Mpk2¹ double mutant larvae exhibit a weak melanotic phenotype under normal food conditions. A "black dot" is seen in the posterior hindgut in approximately 6% of larvae. The number of larvae displaying black dots increases in response to salt stress. The semi-lethality seen in Mpk2¹ mutants in response to salt stress is enhanced by MAPk-Ak2^Δ43.

MAPk-Ak2^Δ43 slightly enhances the melanotic phenotype seen in p38b^d27 larvae under normal food conditions. A "black dot" (BD) is seen in the posterior hindgut in approximately 28% of larvae. However, MAPk-Ak2^Δ43 does not increase the number of p38b^d27 mutant larvae displaying the BD phenotype in salt stress conditions. The semi-lethality seen in p38b^d27 is not enhanced by MAPk-Ak2^Δ43.

(Seisenbacher et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Mapkapk2^Scer\UAS.cSa under the control of the dorsal hindgut expressed driver Scer\GAL4^en-e16E rescues the "black dot" phenotype seen in MAPk-Ak2^Δ43 larvae in response to salt stress.

Ablation of hemocytes (through expression of Mmus\Bax^Scer\UAS.cGb in the hemocytes under the control of Scer\GAL4^He.PZ) does not suppress the "black dot" phenotype seen in MAPk-Ak2^Δ43 mutant larvae.

(Seisenbacher et al., 2011)

Complementation and Rescue Data

Rescued by

MAPk-Ak2^Δ43 is rescued by MAPk-Ak2^+tSa

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is rescued by MAPk-Ak2^UAS.cSa/Scer\GAL4^da.G32

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is rescued by MAPk-Ak2^UAS.cSa/Scer\GAL4^byn.n2

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is rescued by Scer\GAL4^en-e16E/MAPk-Ak2^UAS.cSa

(Seisenbacher et al., 2011)

Partially rescued by

MAPk-Ak2^Δ43 is partially rescued by MAPk-Ak2^UAS.cSa/Scer\GAL4^puc-GAL4E69

(Seisenbacher et al., 2011)

Not rescued by

MAPk-Ak2^Δ43 is not rescued by Scer\GAL4^NP0001/MAPk-Ak2^UAS.cSa

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is not rescued by MAPk-Ak2^UAS.cSa/Scer\GAL4^cad-em459

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is not rescued by Scer\GAL4^da.G32/MAPk-Ak2^K49A.UAS

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is not rescued by Scer\GAL4^byn.n2/MAPk-Ak2^K49A.UAS

(Seisenbacher et al., 2011)

MAPk-Ak2^Δ43 is not rescued by Scer\GAL4^en-e16E/MAPk-Ak2^K49A.UAS

(Seisenbacher et al., 2011)

Comments

Expression of MAPk-Ak2^+tSa rescues the "black dot" phenotype and the lethality seen in MAPk-Ak2^Δ43 larvae as a result of salt stress.

Expression of MAPk-Ak2^Scer\UAS.cSa under the control of either the ubiquitous driver Scer\GAL4^da.G32 or the hindgut driver Scer\GAL4^byn.n2 rescues the "black dot" phenotype seen in MAPk-Ak2^Δ43 as a result of salt stress. Expression of MAPk-Ak2^Scer\UAS.cSa in the midgut under the control of Scer\GAL4^{Myo31DF-NP0001} or Scer\GAL4^cad-em459 is unable to rescue the phenotype. Expression of MAPk-Ak2^Scer\UAS.cSa under the control of the dorsal hindgut expressed driver Scer\GAL4^en-e16E also rescues the phenotype.

Expression of MAPk-Ak2^Scer\UAS.cSa under the control of the dorsal hindgut expressed driver Scer\GAL4^puc-GAL4E69 only mildly rescues the "black dot" phenotype seen in MAPk-Ak2^Δ43 larvae raised on 0.1M NaCl.

Expression of MAPk-Ak2^{K49A.Scer\UAS} under the control of either the ubiquitous driver Scer\GAL4^da.G32 or the hindgut driver Scer\GAL4^byn.n2 fails to rescue the "black dot" phenotype seen in MAPk-Ak2^Δ43 as a result of salt stress. Expression under the control of the dorsal-hindgut expressing driver Scer\GAL4^en-e16E also fails to rescue the phenotype.

(Seisenbacher et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

MAPk-Ak2^Δ43

Δ43

(Seisenbacher et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close