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General Information
Symbol
Dmel\MAPk-Ak2Δ43
Species
D. melanogaster
Name
FlyBase ID
FBal0267165
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Excision of the P{EP} element in P{EP}MAPk-Ak2GE3296 results in deletion of the region downstream of the insertion site, which includes the second exon and most of the third exon of MAPk-Ak2.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

No obvious phenotypes are seen in MAPk-Ak2Δ43 homozygous mutants under normal conditions.

Approximately 38% of MAPk-Ak2Δ43 mutant larvae exhibit a melanotic phenotype in response to strong salt stress (0.2M NaCl). A "black dot" (BD) is seen in the posterior hindgut and the affected hindgut epithelium appears pathologically altered. Approximately 20% of mutants exhibit this phenotype when raised on food containing 0.1M NaCl and no phenotypes are observed when MAPk-Ak2Δ43 mutants are raised on normal food. The dorsal epithelial cells (ECs) containing BDs are apically ruptured, whereas no damage is detected in wild type hindguts under the same conditions. No morphological alterations are seen in MAPk-Ak2Δ43 mutant ECs that do not contain a BD, although a blistering of the surface without damage to the apical membrane is occasionally observed. TUNEL staining reveals that local clusters of apoptotic hindgut ECs are present in mutant larvae under salt stress conditions, and these are not present in controls. The visceral muscles surrounding the hindgut ECs appear unaffected, with a disturbed hindgut musculature only observed in hindguts with large BDs. Loss of epithelial integrity close to BDs is also observed, as determined by more diffuse Avic\GFPNrg-G00305 expression. BDs are only seen in the dorsal hindgut hd compartment.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

MAPk-Ak2Δ43 is a non-enhancer of melanotic mass phenotype | larval stage | nutrition conditional phenotype of p38bd27

Other
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

MAPk-Ak2Δ43 is a non-enhancer of embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype of p38bd27

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Tak12 partially suppresses the "black dot" phenotype seen in the hindgut of MAPk-Ak2Δ43 larvae raised on either 0.1M or 0.2M NaCl.

Tak14 partially suppresses the "black dot" phenotype seen in the hindgut of MAPk-Ak2Δ43 larvae raised on either 0.1M or 0.2M NaCl.

Mkk4G587/Mkk4G414 partially suppresses the "black dot" phenotype seen in the hindgut of MAPk-Ak2Δ43 larvae raised on either 0.1M or 0.2M NaCl.

Expression of bskK53R.Scer\UAS under the control of either Scer\GAL4en-e16E or Scer\GAL4puc-GAL4E69 partially suppresses the "black dot" phenotype seen in MAPk-Ak2Δ43 larvae.

MAPk-Ak2Δ43 Mpk21 double mutant larvae exhibit a weak melanotic phenotype under normal food conditions. A "black dot" is seen in the posterior hindgut in approximately 6% of larvae. The number of larvae displaying black dots increases in response to salt stress. The semi-lethality seen in Mpk21 mutants in response to salt stress is enhanced by MAPk-Ak2Δ43.

MAPk-Ak2Δ43 slightly enhances the melanotic phenotype seen in p38bd27 larvae under normal food conditions. A "black dot" (BD) is seen in the posterior hindgut in approximately 28% of larvae. However, MAPk-Ak2Δ43 does not increase the number of p38bd27 mutant larvae displaying the BD phenotype in salt stress conditions. The semi-lethality seen in p38bd27 is not enhanced by MAPk-Ak2Δ43.

Xenogenetic Interactions
Statement
Reference

Expression of Mmus\Mapkapk2Scer\UAS.cSa under the control of the dorsal hindgut expressed driver Scer\GAL4en-e16E rescues the "black dot" phenotype seen in MAPk-Ak2Δ43 larvae in response to salt stress.

Ablation of hemocytes (through expression of Mmus\BaxScer\UAS.cGb in the hemocytes under the control of Scer\GAL4He.PZ) does not suppress the "black dot" phenotype seen in MAPk-Ak2Δ43 mutant larvae.

Complementation and Rescue Data
Partially rescued by
Comments

Expression of MAPk-Ak2+tSa rescues the "black dot" phenotype and the lethality seen in MAPk-Ak2Δ43 larvae as a result of salt stress.

Expression of MAPk-Ak2Scer\UAS.cSa under the control of either the ubiquitous driver Scer\GAL4da.G32 or the hindgut driver Scer\GAL4byn.n2 rescues the "black dot" phenotype seen in MAPk-Ak2Δ43 as a result of salt stress. Expression of MAPk-Ak2Scer\UAS.cSa in the midgut under the control of Scer\GAL4Myo31DF-NP0001 or Scer\GAL4cad-em459 is unable to rescue the phenotype. Expression of MAPk-Ak2Scer\UAS.cSa under the control of the dorsal hindgut expressed driver Scer\GAL4en-e16E also rescues the phenotype.

Expression of MAPk-Ak2Scer\UAS.cSa under the control of the dorsal hindgut expressed driver Scer\GAL4puc-GAL4E69 only mildly rescues the "black dot" phenotype seen in MAPk-Ak2Δ43 larvae raised on 0.1M NaCl.

Expression of MAPk-Ak2K49A.Scer\UAS under the control of either the ubiquitous driver Scer\GAL4da.G32 or the hindgut driver Scer\GAL4byn.n2 fails to rescue the "black dot" phenotype seen in MAPk-Ak2Δ43 as a result of salt stress. Expression under the control of the dorsal-hindgut expressing driver Scer\GAL4en-e16E also fails to rescue the phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
MAPk-Ak2Δ43
Name Synonyms
Secondary FlyBase IDs
    References (1)