FlyBase Allele Report: Dmel\Piezo[KO]

General Information

Symbol

Dmel\Piezo^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0267720

Feature type

allele

Associated gene

Dmel\Piezo

Associated Insertion(s)

PBac{RB5.WH5}Piezo^KO

Carried in Construct

Also Known As

dmpiezo^KO

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(He et al., 2018, Kim et al., 2012)

Mutagen

FLPase

(Kim et al., 2012)

Progenitor genotype

PBac{RB}Piezo^e00109

(Kim et al., 2012)

PBac{WH}Piezo^f02291

(Kim et al., 2012)

Associated Insertion(s)

PBac{RB5.WH5}Piezo^KO

Cytology

Description

FLPase-mediated recombination between PBac{RB}Piezo^e00109 and PBac{WH}Piezo^f02291 resulting in deletion of all 31 coding exons of Piezo.

(Kim et al., 2012)

Allele components

Component

Use(s)

Inserted element

PBac{RB5.WH5}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | larval stage

(Wang et al., 2025)

axon | larval stage | conditional

(Li et al., 2021)

crop

(Min et al., 2021)

larval multidendritic class III neuron

(Wang et al., 2025)

larval multidendritic class I neuron | conditional

(Li et al., 2021)

Detailed Description

Statement

Reference

Unlike controls, Piezo^KO mutants show increased class III da neuron axonal regeneration after injury.

(Li et al., 2021)

Piezo^KO adults exhibit crop distension, increased food intake, increased feeding bout duration and increased defecation (fecal spots); abdomen bursting does rarely occur,

(Min et al., 2021)

Piezo^KO mutant adult females have no difference in preference for softer plain, or 100 mM sucrose-containing, agarose for egg laying, compared to controls.

(Wu et al., 2019)

Piezo^KO mutant adults have no difference in spiking of mechanosensory neurons of the labellar taste bristles or labellar taste pegs in response to mechanical stimuli, compared to controls.

(Zhou et al., 2019)

Piezo^KO mutant adults do not show any defects in sensing food hardness and like the wild-type flies prefer softer food with lower sugar content (0.5mM sucrose, 0.2% agarose) to a hard food with higher sugar content (1mM sucrose, 2% agarose) in a binary choice assay.

(Jeong et al., 2016)

Piezo^KO mutant adults show normal preference for less solid sucrose-agarose food than a harder one (higher agarose conc., same sucrose content) one in a two-way food choice assay.

(Zhang et al., 2016)

Piezo^KO/Piezo^KO third instar larvae have normal locomotor turning frequency, but show significantly reduced mechanical nociception (response to harsh touch).

(Gorczyca et al., 2014)

The percentage of Piezo^KO/Piezo^KO third instar larvae showing rolling behavior in response to nociceptive mechanical stimuli (2.5, 5, 12 and 47mN) is significantly reduced compared to wild type.

(Guo et al., 2014)

Piezo^KO mutant larvae do not have alterations in defecation rhythm compared to controls.

(Zhang et al., 2014)

Piezo^KO mutants exhibit a wild-type behavioral response to 500Hz sound.

(Zhang et al., 2013)

Mutants do not show a lack of coordination or a defect in bristle mechanoreceptor potential.

Piezo^KO homozygous larvae have a severe mechanical nociception response deficit over a wide range of stimulation intensities. Piezo^KO/Df(2L)Exel7034 trans-heterozygous larvae show a similar defect.

Piezo^KO larvae are indistinguishable from wild type in assays for responses to high temperature or innocuous gentle touch.

Mechanical stimulation of isolated ppk-positive neurons from Piezo^KO larvae does not result in a current, whereas stimulation of these neurons from wild type larvae does generate a current.

(Kim et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Piezo^KO has abnormal pain response | third instar larval stage phenotype, enhanceable by ppk26¹/ppk26¹

(Guo et al., 2014)

Piezo^KO has abnormal pain response | third instar larval stage phenotype, enhanceable by ppk26^Gal4/ppk26^Gal4

(Guo et al., 2014)

NOT Enhanced by

Statement

Reference

Piezo^KO has abnormal touch response phenotype, non-enhanceable by pain^EP2451/pain^EP2451

(Kim et al., 2012)

Suppressed by

Statement

Reference

Piezo^KO has abnormal wound healing | recessive | larval stage phenotype, suppressible by Scer\GAL4^19-12/Hsap\PIEZO1^UAS.cSa

(Wang et al., 2025)

Piezo^KO has abnormal neuroanatomy | recessive | larval stage phenotype, suppressible by Scer\GAL4^19-12/Hsap\PIEZO1^UAS.cSa

(Wang et al., 2025)

Piezo^KO has increased rate of feeding behavior | recessive | adult stage phenotype, suppressible by Scer\GAL4^Piezo.1.0/TrpA1^UAS.(B).cKa

(Min et al., 2021)

Piezo^KO has increased size | recessive | adult stage phenotype, suppressible by Scer\GAL4^Piezo.1.0/TrpA1^UAS.(B).cKa

(Min et al., 2021)

NOT suppressed by

Statement

Reference

Piezo^KO has abnormal wound healing | recessive | larval stage phenotype, non-suppressible by Hsap\PIEZO1^4PA.UAS/Scer\GAL4^19-12

(Wang et al., 2025)

Piezo^KO has abnormal neuroanatomy | recessive | larval stage phenotype, non-suppressible by Hsap\PIEZO1^4PA.UAS/Scer\GAL4^19-12

(Wang et al., 2025)

Piezo^KO has abnormal touch response phenotype, non-suppressible by pain^EP2451/pain^EP2451

(Kim et al., 2012)

Enhancer of

Statement

Reference

Piezo[+]/Piezo^KO is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Piezo^KO/Piezo^KO is an enhancer of abnormal pain response | third instar larval stage phenotype of ppk26^Gal4

(Guo et al., 2014)

Piezo^KO/Piezo^KO is an enhancer of abnormal pain response | third instar larval stage phenotype of ppk26¹

(Guo et al., 2014)

NOT Enhancer of

Statement

Reference

Piezo^KO/Piezo^KO is a non-enhancer of abnormal touch response phenotype of pain^EP2451

(Kim et al., 2012)

NOT Suppressor of

Statement

Reference

Piezo^KO/Piezo^KO is a non-suppressor of abnormal touch response phenotype of pain^EP2451

(Kim et al., 2012)

Other

Statement

Reference

Piezo^KO, mys¹ has abnormal neuroanatomy | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, mys¹ has abnormal wound healing | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, Rtca^Δ has abnormal neuroanatomy | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, Rtca^Δ has abnormal wound healing | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, zip¹ has abnormal neuroanatomy | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, zip¹ has abnormal wound healing | larval stage phenotype

(Wang et al., 2025)

Piezo[+]/Piezo^KO, pain^EP2451 has abnormal touch response phenotype

(Kim et al., 2012)

Piezo^KO, pain^EP2451/pain[+] has abnormal touch response phenotype

(Kim et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Piezo^KO has axon | larval stage phenotype, suppressible by Scer\GAL4^19-12/Hsap\PIEZO1^UAS.cSa

(Wang et al., 2025)

Piezo^KO has larval multidendritic class III neuron phenotype, suppressible by Scer\GAL4^19-12/Hsap\PIEZO1^UAS.cSa

(Wang et al., 2025)

Piezo^KO has crop phenotype, suppressible by Scer\GAL4^Piezo.1.0/TrpA1^UAS.(B).cKa

(Min et al., 2021)

NOT suppressed by

Statement

Reference

Piezo^KO has larval multidendritic class III neuron phenotype, non-suppressible by Hsap\PIEZO1^4PA.UAS/Scer\GAL4^19-12

(Wang et al., 2025)

Piezo^KO has axon | larval stage phenotype, non-suppressible by Hsap\PIEZO1^4PA.UAS/Scer\GAL4^19-12

(Wang et al., 2025)

Enhancer of

Statement

Reference

Piezo[+]/Piezo^KO is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Other

Statement

Reference

Piezo^KO, mys¹ has axon | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, mys¹ has larval multidendritic class III neuron phenotype

(Wang et al., 2025)

Piezo^KO, Rtca^Δ has axon | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, Rtca^Δ has larval multidendritic class III neuron phenotype

(Wang et al., 2025)

Piezo^KO, zip¹ has axon | larval stage phenotype

(Wang et al., 2025)

Piezo^KO, zip¹ has larval multidendritic class III neuron phenotype

(Wang et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Acute activation of Piezo-positive neurons, by expressing TrpA1^UAS.(B).cKa under the control of Scer\GAL4^Piezo.1.0 and activating it 30[o]C, suppresses the increased feeding and crop distention observed in Piezo^KO adults.

(Min et al., 2021)

The percentage of Piezo^KO/Piezo^KO;ppk26¹/ppk26¹ or Piezo^KO/Piezo^KO;ppk26^Gal4/ppk26^Gal4 double mutant third instar larvae showing rolling behavior in response to nociceptive mechanical stimuli (47mN) is significantly reduced compared to either single mutant alone.

(Guo et al., 2014)

pain^EP2451, Piezo^KO double mutants have a mechanical nociception response deficit that is comparable to either single mutant.

Larvae that are heterozygous for both pain^EP2451 and Piezo^KO show a mechanical nociception response deficit whereas each separate heterozygote is normal.

(Kim et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Piezo^KO is rescued by Scer\GAL4^Piezo.1.0/Piezo^UAS.EGFP

(Min et al., 2021)

Piezo^KO is rescued by Piezo^UAS.EGFP/Scer\GAL4^ppk.PG

(Kim et al., 2012)

Not rescued by

Piezo^KO is not rescued by Piezo^UAS.EGFP/Scer\GAL4^esg.PU

(Min et al., 2021)

Comments

Scer\GAL4^ppk.PG-mediated expression of Piezo^{Scer\UAS.T:Avic\GFP-EGFP} rescues the mechanical nociception phenotype of Piezo^KO larvae.

(Kim et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

58770

w^*; PBac{RB5.WH5}Piezo^KO

95298

w^*; PBac{RB5.WH5}Piezo^KO P{neoFRT}40A; M{UAS-Piezo.2306MYC.FLAG}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

DmPiezo^KO

(Li et al., 2021)

DmPiezoKO

(Song et al., 2019)

Dmpiezo KO

(Kim et al., 2012)

Dmpiezo^KO

(Zhang et al., 2020)

Piezo KO

(Min et al., 2021)

Piezo^KO

(Stricker et al., 2025, Wang et al., 2025, Zhao et al., 2025, Schoofs et al., 2024, Zhao et al., 2024, Gong et al., 2022, Beqja et al., 2020, Feuillette et al., 2020, Jiang et al., 2020, Wu et al., 2019, He et al., 2018, Jeong et al., 2016)

dPiezo^KO

(Chen et al., 2018)

dmpiezo^KO

(Zhang et al., 2013)

piezo (KO)

(Tian et al., 2023)

piezo^K.O

(Oh et al., 2021)

piezo^KO

(Guo et al., 2025, Luedke et al., 2024, Yamanouchi et al., 2023, Li et al., 2022, Liu et al., 2022, Xie et al., 2022, Li and Montell, 2021, Wang et al., 2020, Zhang et al., 2020, Shao et al., 2019, Zhou et al., 2019, Li et al., 2016, Zhang et al., 2016, Ramdya et al., 2015, Guo et al., 2014, Zhang et al., 2014)

piezo^ko

(Gorczyca et al., 2014)

piezoKO

(Ohyama et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (39)

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