FlyBase Allele Report: Dmel\grk[6]

General Information

Symbol

Dmel\grk⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0270011

Feature type

allele

Associated gene

Dmel\grk

Associated Insertion(s)

Carried in Construct

Also Known As

grk^WG, grk^WG41

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neuman-Silberberg and Schupbach, 1996, Neuman-Silberberg and Schupbach, 1993, Schupbach and Wieschaus, 1991, Schupbach, 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. Amino acid replacement: R221Q.

(Schupbach, 2001.10.19)

Mutation stated to be R to E, but see Schupbach, 2001.10.19, personal communication to FlyBase for correction. Nucleotide substitution: G2217A.

(Thio et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,431,900..8,431,900 [-]

Nucleotide change:

G8431900A

Reported nucleotide change:

G2217A

Amino acid change:

R222Q | grk-PA

Reported amino acid change:

R221Q

(Thio et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Schupbach and Wieschaus, 1991)

female sterile | recessive

(Neuman-Silberberg and Schupbach, 1993)

lethal | maternal effect | recessive

(Roth and Schupbach, 1994)

Phenotype Manifest In

dorsal appendage

(Peri et al., 1999, Schupbach, 1987)

dorsal appendage | maternal effect

(Roth and Schupbach, 1994)

egg

(Peri et al., 1999, Forlani et al., 1993)

egg chamber

(Peri et al., 1999)

egg operculum

(Schupbach, 1987)

embryo | ventral

(Schupbach, 1987)

embryonic/first instar larval cuticle (with grk²)

(Morisato, 2001)

follicle cell

(Gonzalez-Reyes et al., 1995)

ventral furrow

(Schupbach, 1987)

ventral furrow | ectopic (with grk²)

(Morisato, 2001)

ventral furrow | increased number (with grk²)

(Morisato, 2001)

Detailed Description

Statement

Reference

Embryos derived from grk²/grk⁶ females gastrulate with two distinct ventral furrows. Cuticles show an expansion of ventral structures.

(Morisato, 2001)

grk⁶/grk³ females produce weakly ventralized egg chambers. Eggs laid have lost their dorsalmost fates and have a single medially fused dorsal appendage.

(Peri et al., 1999)

Posterior follicle cells show an abnormal shape and behaviour.

(Gonzalez-Reyes et al., 1995)

grk³/grk⁶ causes strong ventralization of embryos: 71% of eggs have reduced and 22% of eggs have no dorsal appendages. 2% of the eggs contain embryos.

(Roth and Schupbach, 1994)

grk⁶ homozygous females also heterozygous or homozygous for fs(1)K10¹³ or fs(1)K10¹ produce eggs with a ventralised phenotype.

(Forlani et al., 1993)

Embryos from double mutants with sqd^ix43 are weakly ventralised.

(Kelley, 1993)

Follicular epithelium of mutant egg chambers has holes.

(Goode et al., 1992)

Reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle at both ends of eggs, embryos ventralized.

(Schupbach and Wieschaus, 1991)

An enlarged mass of mesodermal cells invaginates on the ventral side of the embryo, the enlarged mesoderm is often organised into two ventral furrows. Organisation is lost in later stages of development and a mass of mesodermal cells fills the ventral half of the embryo. Respiratory appendages are fused and shortened and adjacent operculum is reduced.

(Schupbach, 1987)

phenotype of eggs and embryos: mostly intermediate but can vary from weak to strong

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

grk⁶/grk[+] is an enhancer of embryonic epidermis phenotype of cact^E10

(Wakabayashi-Ito et al., 2001)

grk⁶ is an enhancer of dorsal appendage phenotype of Lis-1^k11702

(Lei and Warrior, 2000)

NOT Enhancer of

Statement

Reference

grk⁶ is a non-enhancer of embryonic epidermis phenotype of fus¹

(Wakabayashi-Ito et al., 2001)

Suppressor of

Statement

Reference

grk⁶ is a suppressor | partially of dorsal appendage phenotype of Scer\GAL4^55B, rho^UAS.cGa

(Sapir et al., 1998)

grk⁶ is a suppressor | partially of ventral denticle belt phenotype of Scer\GAL4^55B, rho^UAS.cGa

(Sapir et al., 1998)

grk⁶ is a suppressor | partially of larval dorsal hair phenotype of Scer\GAL4^55B, rho^UAS.cGa

(Sapir et al., 1998)

Other

Statement

Reference

da², grk⁶/grk[+] has follicle cell phenotype

(Smith et al., 2002)

da², grk⁶ has follicle cell phenotype

(Smith et al., 2002)

Lis-1^3.1.2, grk⁶ has dorsal appendage phenotype

(Lei and Warrior, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35504

grk⁶ Adc^b-1 bw¹ speck¹/CyO, l(2)DTS100¹

Notes on Origin

Discoverer

Comments

Intermediate grk allele.

(Schupbach, 1987)

"Nucleotide substitution: G?A." was stated as revision. "Amino acid replacement: R221Q." was stated as revision.

(Schupbach, 2001.10.19)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

grk⁶

grk^WG12

(Peri et al., 1999)

grk^WG41

(Smith et al., 2002, Thio et al., 2000, Reich et al., 1999, Sapir et al., 1998, Forlani et al., 1993)

grk^WG

(Lin et al., 2007, Morisato, 2001, Pathirana et al., 2001, Wakabayashi-Ito et al., 2001, Zhao et al., 2000, Zhao et al., 2000, Zhao and Bownes, 1999, Sen et al., 1998, Neuman-Silberberg and Schupbach, 1996, Gonzalez-Reyes et al., 1995, Hong and Hashimoto, 1995, Roth et al., 1995, Roth and Schupbach, 1994, Kelley, 1993, Neuman-Silberberg and Schupbach, 1993, Clifford and Schupbach, 1989, Schupbach, 1987)

Name Synonyms

Secondary FlyBase IDs

FBal0005214
FBal0191238

References (28)

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