FlyBase Allele Report: Scer\GAL4[Hk-UH3]

General Information

Symbol

Scer\GAL4^Hk-UH3

Species

S. cerevisiae

Name

FlyBase ID

FBal0277817

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}Hk^UH3

Carried in Construct

Also Known As

UH3-Gal4

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}Hk^UH3

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:10,257,539..10,257,539 [-]

(Farkas et al., 2024, Nikonova et al., 2024, Schneider et al., 2023, Wishard et al., 2023, Nikonova et al., 2022, Liao et al., 2020, González-Morales et al., 2019, Katti et al., 2017, Chatterjee et al., 2016, Kooij et al., 2016, Liao et al., 2016, Firdaus et al., 2015, Viswanathan et al., 2015, Molnár et al., 2014, Singh et al., 2014, Katzemich et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing disc | restricted

(Singh et al., 2014)

pupal stage P6 -- P7

organism | ubiquitous

(Singh et al., 2014)

pupal stage P7 -- adult stage

indirect flight muscle cell

(Singh et al., 2014)

adult stage

indirect flight muscle cell

(Katzemich et al., 2012)

Additional Information

Statement

Reference

ScerGAL4^UH3 drives expression in a complex pattern in the wing disc, but is not observed in the presumptive notum or myoblasts. ScerGAL4^UH3 drives expression ubiquitously in early pupae; by 35 hours APF, expression is more pronounced in the developing indirect flight muscles (IFMs), and is restricted to the IFMs by from 55 hours APF. ScerGAL4^UH3 continues to drive expression in the indirect flight muscles in adults.

(Singh et al., 2014)

Marker for

indirect flight muscle cell

(Singh et al., 2014)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with chic^UAS.cJa

(Kooij et al., 2016)

abnormal flight, with sals^JF01109

(Farkas et al., 2024)

abnormal flight, with sals^KK112869

(Farkas et al., 2024)

abnormal flight, with TpnC41C^JF02379

(Singh et al., 2014)

decreased size, with Rbfox1^UAS.cUa

(Nikonova et al., 2022)

decreased size, with tmod^UAS.cBa

(Farkas et al., 2024)

decreased size | adult stage, with sals^JF01109

(Farkas et al., 2024)

decreased size | adult stage, with sals^KK112869

(Farkas et al., 2024)

flightless, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

flightless, with DAAM^KK102786, Dcr-2^UAS.cDa

(Molnár et al., 2014)

flightless, with DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

flightless, with mir-9a^UAS.pLUC

(Katti et al., 2017)

flightless, with Obsc^GD14839

(Katzemich et al., 2012)

flightless, with sals^JF01109

(Farkas et al., 2024)

flightless, with sals^KK112869

(Farkas et al., 2024)

flightless, with TpnC4^GD7719

(Singh et al., 2014)

flightless, with up^HMS00743

(Singh et al., 2014)

flightless, with wupA^JF02172

(Singh et al., 2014)

flightless, with wupA^UAS.L9, wupA^hdp-3

(Singh et al., 2014)

increased size, with sals^UAS.Tag:FLAG

(Farkas et al., 2024)

lethal - all die during pupal stage, with chic^HMS00550

(Kooij et al., 2016)

visible | adult stage, with mir-9a^UAS.pLUC

(Katti et al., 2017)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with mir-9a^UAS.pLUC

(Katti et al., 2017)

dorsal longitudinal indirect flight muscle cell, with sls^GD9680

(Katti et al., 2017)

dorsal longitudinal indirect flight muscle cell, with up^GD12132

(Katti et al., 2017)

H zone | adult stage, with Obsc^GD14839

(Katzemich et al., 2012)

indirect flight muscle, with sals^UAS.Tag:FLAG

(Farkas et al., 2024)

indirect flight muscle, with tmod^UAS.cBa

(Farkas et al., 2024)

indirect flight muscle | adult stage, with sals^JF01109

(Farkas et al., 2024)

indirect flight muscle | adult stage, with sals^KK112869

(Farkas et al., 2024)

indirect flight muscle cell, with chic^UAS.cJa

(Kooij et al., 2016)

indirect flight muscle cell, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

indirect flight muscle cell, with Rbfox1^UAS.cUa

(Nikonova et al., 2022)

indirect flight muscle cell, with tmod^NIG.1539R

(Molnár et al., 2014)

indirect flight muscle cell, with TpnC4^GD7719

(Singh et al., 2014)

indirect flight muscle cell, with up^HMS00743

(Singh et al., 2014)

indirect flight muscle cell, with wupA^JF02172

(Singh et al., 2014)

indirect flight muscle cell, with wupA^UAS.L9, wupA^hdp-3

(Singh et al., 2014)

indirect flight muscle cell, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

indirect flight muscle cell | adult stage, with Obsc^GD14839

(Katzemich et al., 2012)

M band, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

M band, with tmod^UAS.cBa

(Farkas et al., 2024)

M band | adult stage, with sals^JF01109

(Farkas et al., 2024)

M band | adult stage, with sals^KK112869

(Farkas et al., 2024)

muscle cell, with Rbfox1^UAS.cUa

(Nikonova et al., 2022)

myofibril, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

myofibril, with Rbfox1^UAS.cUa

(Nikonova et al., 2022)

myofibril, with tmod^NIG.1539R

(Molnár et al., 2014)

myofibril, with tmod^UAS.cBa

(Farkas et al., 2024)

myofibril | adult stage, with mir-9a^UAS.pLUC

(Katti et al., 2017)

myofibril | adult stage, with Obsc^GD14839

(Katzemich et al., 2012)

myofibril | adult stage, with sals^KK112869

(Farkas et al., 2024)

myofibril | adult stage, with up^GD12132

(Katti et al., 2017)

myofilament | adult stage, with sals^KK112869

(Farkas et al., 2024)

sarcomere, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

sarcomere, with Rbfox1^UAS.cUa

(Nikonova et al., 2022)

sarcomere, with sals^UAS.Tag:FLAG

(Farkas et al., 2024)

sarcomere, with tmod^NIG.1539R

(Molnár et al., 2014)

sarcomere, with tmod^UAS.cBa

(Farkas et al., 2024)

sarcomere, with TpnC4^GD7719

(Singh et al., 2014)

sarcomere, with up^HMS00743

(Singh et al., 2014)

sarcomere, with wupA^JF02172

(Singh et al., 2014)

sarcomere | adult stage, with chic^UAS.cJa

(Kooij et al., 2016)

sarcomere | adult stage, with mir-9a^UAS.pLUC

(Katti et al., 2017)

sarcomere | adult stage, with sals^JF01109

(Farkas et al., 2024)

sarcomere | adult stage, with sals^KK112869

(Farkas et al., 2024)

sarcomere | adult stage, with sls^GD9680

(Katti et al., 2017)

sarcomere | adult stage, with up^GD12132

(Katti et al., 2017)

sarcomere | adult stage, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

striated muscle myosin thick filament, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

striated muscle thin filament, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

striated muscle thin filament, with tmod^UAS.cBa

(Farkas et al., 2024, Molnár et al., 2014)

striated muscle thin filament | adult stage, with chic^UAS.cJa

(Kooij et al., 2016)

striated muscle thin filament | adult stage, with sals^JF01109

(Farkas et al., 2024)

striated muscle thin filament | adult stage, with sals^KK112869

(Farkas et al., 2024)

wing, with mir-9a^UAS.pLUC

(Katti et al., 2017)

Z disc, with DAAM^Ex1, DAAM^{RNAi.UAS.T129M}, Dcr-2^UAS.cDa

(Molnár et al., 2014)

Z disc, with tmod^UAS.cBa

(Farkas et al., 2024)

Z disc | adult stage, with sals^JF01109

(Farkas et al., 2024)

Z disc | adult stage, with sals^KK112869

(Farkas et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has flightless phenotype, suppressible | partially by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has flightless phenotype, suppressible | partially by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Suppressor of

Statement

Reference

up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3 is a suppressor | partially of flightless phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3 is a suppressor | partially of flightless phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has sarcomere | adult stage phenotype, suppressible by Mhc^2B/Mhc^2B

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has myofibril | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has sarcomere | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has myofibril | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has sarcomere | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by Mhc^2B/Mhc^2B

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has myofibril | adult stage phenotype, suppressible by Mhc^2B/Mhc^2B

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, tmod^NIG.1539R has sarcomere phenotype, suppressible by DAAM^Ex1

(Molnár et al., 2014)

Scer\GAL4^Hk-UH3, tmod^NIG.1539R has indirect flight muscle cell phenotype, suppressible by DAAM^Ex1

(Molnár et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^Hk-UH3, tmod^UAS.cBa has striated muscle thin filament phenotype, non-suppressible by DAAM^UASp.cMa, Scer\GAL4^Hk-UH3

(Molnár et al., 2014)

Suppressor of

Statement

Reference

up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3 is a suppressor of dorsal longitudinal indirect flight muscle cell phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3 is a suppressor of myofibril | adult stage phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3 is a suppressor of sarcomere | adult stage phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3 is a suppressor of dorsal longitudinal indirect flight muscle cell phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3 is a suppressor of myofibril | adult stage phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3 is a suppressor of sarcomere | adult stage phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

NOT Suppressor of

Statement

Reference

DAAM^UASp.cMa, Scer\GAL4^Hk-UH3 is a non-suppressor of striated muscle thin filament phenotype of Scer\GAL4^Hk-UH3, tmod^UAS.cBa

(Molnár et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Zasp52^{Stop143.UAS.Tag:FLAG}/Scer\GAL4^Hk-UH3 partially rescues Zasp52^MI02988/Df(2R)BSC427

(Liao et al., 2020)

wupA^UAS.L9/Scer\GAL4^Hk-UH3 partially rescues wupA^hdp-3

(Singh et al., 2014)

Fails to rescue

Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}/Scer\GAL4^Hk-UH3 fails to rescue Zasp52^MI02988/Df(2R)BSC427

(Liao et al., 2016)

Zasp52^{LIM234.UAS.FSH}/Scer\GAL4^Hk-UH3 fails to rescue Zasp52^MI02988/Df(2R)BSC427

(Liao et al., 2016)

Zasp52^{UAS.PR.Tag:FLAG}/Scer\GAL4^Hk-UH3 fails to rescue Zasp52^MI00979/Df(2R)BSC427

(Liao et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Hk-UH3

Scer\GAL4^UH3

Name Synonyms

Secondary FlyBase IDs

References (16)

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