FlyBase Allele Report: Dmel\trbl[UAS.Tag:FLAG]

General Information

Symbol

Dmel\trbl^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0284617

Feature type

allele

Associated gene

Dmel\trbl

Associated Insertion(s)

Carried in Construct

P{UAS-FLAG-trbl.WT}

Also Known As

UAS-FLAG-trbl

Key Links

Transgenic product class

wild_type

(Masoner et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Masoner et al., 2013)

Mutagen

in vitro construct

(Masoner et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-FLAG-trbl.WT}

Cytology

Description

UASt regulatory sequences drive expression of trbl which is tagged at the N-terminal end with Tag:FLAG.

(Masoner et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

trbl

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | oogenesis, with Scer\GAL4^slbo.2.6

(Fischer et al., 2017)

abnormal developmental rate, with Scer\GAL4^ppl.PP

(Das et al., 2014)

abnormal developmental rate | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

abnormal size | adult stage | female limited, with Scer\GAL4^ppl.PP

(Das et al., 2014)

abnormal size | larval stage, with Scer\GAL4^ppl.PP

(Das et al., 2014)

abnormal size | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

decreased cell size, with Scer\GAL4^ppl.PP

(Das et al., 2014)

decreased cell size | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

increased cell size, with Scer\GAL4^e16A

(Das et al., 2014)

visible, with Scer\GAL4^ap-md544

(Das et al., 2014)

visible, with Scer\GAL4^e16A

(Das et al., 2014)

visible, with Scer\GAL4^e22c

(Masoner et al., 2013)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Fischer et al., 2017, Masoner et al., 2013)

crossvein, with Scer\GAL4^e22c

(Masoner et al., 2013)

embryonic/larval fat body | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

fat cell | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

larva, with Scer\GAL4^r4

(Fischer et al., 2017)

lipid droplet | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

nucleus | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

wing, with Scer\GAL4^ap-md544

(Das et al., 2014)

wing, with Scer\GAL4^e16A

(Das et al., 2014)

wing disc posterior compartment, with Scer\GAL4^e22c

(Masoner et al., 2013)

wing hair, with Scer\GAL4^e16A

(Das et al., 2014)

wing hair, with Scer\GAL4^en-e16E

(Fischer et al., 2017)

Detailed Description

Statement

Reference

Larvae expressing trbl^UAS.Tag:FLAG under the control of Scer\GAL4^r4 show a significant decrease in body weight; larval fat body cells, their nuclei and lipid droplets are significantly smaller than in control; there is a delay to pupariation.

The expression of trbl^UAS.Tag:FLAG under the control of Scer\GAL4^slbo.2.6 induces a severe block in border cell migration.

The expression of trbl^UAS.Tag:FLAG under the control of Scer\GAL4^en-e16E leads to a significant reduction in wing size:trichome density ratio, despite of no strong differences in wing size, as compared to controls.

(Fischer et al., 2017)

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} in the posterior wing compartment under the control of Scer\GAL4^e16A results in an increase in cell size (detectable as a decrease in the density of trichomes) and a decrease in overall wing size.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} in the fat body under the control of Scer\GAL4^ppl.PP results in a significant reduction in body weight at the larval stages and in two-day old female adults compared with age-matched controls. Males are not appreciably affected. The larval fat body cells and nuclei are smaller than in wild type controls. Pupariation and eclosion are delayed.

trbl^{Scer\UAS.T:Zzzz\FLAG} expression in the fat body under the control of Scer\GAL4^ppl.PP leads to a significant increase in circulating levels of glucose and trehalose. There is a significant decrease in total triglyceride levels.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} in the eye and head under the control of Scer\GAL4^GMR.long has no effect on head size compared with controls.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ap-md544 causes the wing to bend up.

(Das et al., 2014)

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^slbo.2.6 partially blocks border cell migration.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^e22c results in crossvein defects and an increase in cells size in the posterior wing compartment.

(Masoner et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has increased cell size phenotype, suppressible by S6k^UAS.cUa, Scer\GAL4^e16A

(Das et al., 2014)

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has increased cell size phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^e16A

(Das et al., 2014)

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has visible phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^e16A

(Das et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has increased cell size phenotype, non-suppressible by Pten^JF01859, Scer\GAL4^e16A

(Das et al., 2014)

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has increased cell size phenotype, non-suppressible by Pi3K92E^UAS.Exel, Scer\GAL4^e16A

(Das et al., 2014)

Enhancer of

Statement

Reference

Scer\GAL4^ap-md544/trbl^UAS.Tag:FLAG is an enhancer of visible phenotype of Scer\GAL4^ap-md544, foxo^UAS.cPa

(Das et al., 2014)

Suppressor of

Statement

Reference

trbl^UAS.Tag:FLAG, Scer\GAL4^GMR.long is a suppressor of abnormal size phenotype of Akt^UAS.Exel, Scer\GAL4^GMR.long

(Das et al., 2014)

trbl^UAS.Tag:FLAG, Scer\GAL4^Mef2.PR is a suppressor of lethal | heat sensitive phenotype of Akt^UAS.Exel, Scer\GAL4^Mef2.PR

(Das et al., 2014)

trbl^UAS.Tag:FLAG, Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Akt^UAS.Exel, Scer\GAL4^ap-md544

(Das et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has wing phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^e16A

(Das et al., 2014)

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has wing hair phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^e16A

(Das et al., 2014)

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has wing hair phenotype, suppressible by S6k^UAS.cUa, Scer\GAL4^e16A

(Das et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has wing hair phenotype, non-suppressible by Pten^JF01859, Scer\GAL4^e16A

(Das et al., 2014)

Scer\GAL4^e16A, trbl^UAS.Tag:FLAG has wing hair phenotype, non-suppressible by Pi3K92E^UAS.Exel, Scer\GAL4^e16A

(Das et al., 2014)

Enhancer of

Statement

Reference

Scer\GAL4^ap-md544/trbl^UAS.Tag:FLAG is an enhancer of wing phenotype of Scer\GAL4^ap-md544, foxo^UAS.cPa

(Das et al., 2014)

Suppressor of

Statement

Reference

trbl^UAS.Tag:FLAG, Scer\GAL4^GMR.long is a suppressor of adult head phenotype of Akt^UAS.Exel, Scer\GAL4^GMR.long

(Das et al., 2014)

trbl^UAS.Tag:FLAG, Scer\GAL4^Mef2.PR is a suppressor of larval muscle system phenotype of Akt^UAS.Exel, Scer\GAL4^Mef2.PR

(Das et al., 2014)

trbl^UAS.Tag:FLAG, Scer\GAL4^ppl.PP is a suppressor of embryonic/larval fat body phenotype of Akt^UAS.Exel, Scer\GAL4^ppl.PP

(Das et al., 2014)

trbl^UAS.Tag:FLAG, Scer\GAL4^e16A is a suppressor of wing hair phenotype of Pten^JF01859, Scer\GAL4^e16A

(Das et al., 2014)

trbl^UAS.Tag:FLAG, Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Akt^UAS.Exel, Scer\GAL4^ap-md544

(Das et al., 2014)

NOT Suppressor of

Statement

Reference

trbl^UAS.Tag:FLAG, Scer\GAL4^e16A is a non-suppressor of wing hair phenotype of S6k^STDE.UAS, Scer\GAL4^e16A

(Das et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Akt1^{Scer\UAS.Exel} suppresses the reductions in trichome density and overall wing size seen when trbl^{Scer\UAS.T:Zzzz\FLAG} is expressed under the control of Scer\GAL4^e16A.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} suppresses the increase in head size seen when Akt1^{Scer\UAS.Exel} is expressed in the eye and head under the control of Scer\GAL4^GMR.long.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} suppresses the increase in fibre breadth seen in larval muscles when Akt1^{Scer\UAS.Exel} is expressed under the control of Scer\GAL4^Mef2.PR.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} suppresses the increase in cell and nucleus size seen when Akt1^{Scer\UAS.Exel} is expressed under in the larval fat body the control of Scer\GAL4^ppl.PP.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} suppresses the increase in trichome density seen in the wings of flies expressing Pten^JF01859 under the control of Scer\GAL4^e16A. The resulting flies exhibit a significant decrease in trichome density compared with controls, as is seen in trbl^{Scer\UAS.T:Zzzz\FLAG} alone.

Expression of Pi3K92E^{Scer\UAS.Exel} does not suppress the reduction in trichome density seen in the wings of flies expressing trbl^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^e16A.

Expression of S6k^Scer\UAS.cUa suppresses the reduction in trichome density seen in the wings of flies expressing trbl^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^e16A. The resulting flies exhibit a significant increase in trichome density, as is seen in S6k^Scer\UAS.cUa alone.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} suppresses the downward wing bending phenotype seen when Akt1^{Scer\UAS.Exel} is expressed under the control of Scer\GAL4^ap-md544, resulting in a nearly flat wing blade.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} suppresses the bending downward of the wing blade seen when S6k^{STDE.Scer\UAS} is expressed under the control of Scer\GAL4^ap-md544, resulting in a flat wing blade.

Expression of trbl^{Scer\UAS.T:Zzzz\FLAG} enhances the curved upward wing phenotype seen when foxo^Scer\UAS.cPa is expressed under the control of Scer\GAL4^ap-md544.

(Das et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

trbl^{Scer\UAS.T:Zzzz\FLAG}

trbl^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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