FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4Gr43a-GAL4
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General Information
Symbol
Scer\GAL4Gr43a-GAL4
Species
S. cerevisiae
Name
FlyBase ID
FBal0290234
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{GAL4}Gr43aGAL4
Carried in Construct
Also Known As
Gr43aGAL4, Gr43a-GAL4
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Miyamoto et al., 2012)
Progenitor genotype
Associated Insertion(s)
TI{GAL4}Gr43aGAL4
Cytology
Description

The Gr43a coding region has been replaced by sequence encoding a GAL4 driver, resulting in GAL4 being expressed under the control of endogenous Gr43a regulatory sequences.

(Miyamoto et al., 2012)
Allele components
Component
Use(s)
Inserted element
TI{GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval gustatory receptor neuron of the posterior pharyngeal sense organ | subset
      (Mishra et al., 2013)
      larval gustatory receptor neuron of the dorsal pharyngeal sense organ | subset
      (Mishra et al., 2013)
      larval gustatory receptor neuron of the ventral pharyngeal sense organ | subset
      (Mishra et al., 2013)
      embryonic/larval ventricular ganglion | restricted
      (Mishra et al., 2013)
      larval protocerebrum | restricted
      (Mishra et al., 2013)
      embryonic/larval mushroom body | restricted
      (Mishra et al., 2013)
      larval central nervous system | restricted
      (Mishra et al., 2013)
      larval gnathal ganglion | restricted
      (Mishra et al., 2013)
      larval gustatory receptor neuron of the ventral pharyngeal sense organ
      (Mishra et al., 2018)
      larval gustatory receptor neuron of the dorsal pharyngeal sense organ
      (Mishra et al., 2018)
      adult stage
      sugar-sensing neuron of taste bristle | subset of prothoracic leg taste bristle
      (Miyamoto et al., 2012)
      sugar-sensing neuron of taste bristle | subset of labral sense organ neuron
      (Miyamoto et al., 2012)
      ventral cibarial sense organ neuron
      (Miyamoto et al., 2012)
      neuron | subset of labellum | faint
      (Miyamoto et al., 2012)
      adult labral nerve
      (Miyamoto et al., 2012)
      adult gnathal ganglion | restricted
      (Miyamoto et al., 2012)
      neuron | subset of adult proventriculus
      (Miyamoto et al., 2012)
      neuron | subset of posterior superior lateral protocerebrum
      (Miyamoto et al., 2012)
      neuron | subset of uterus
      (Miyamoto et al., 2012)
      adult foregut | restricted
      (Miyamoto et al., 2012)
      adult midgut | restricted
      (Miyamoto et al., 2012)
      adult subesophageal zone | restricted
      (Miyamoto et al., 2012)
      Additional Information
      Statement
      Reference

      Drives expression in sugar-sensing neurons of the larval dorsal, posterior, and ventral pharyngeal sense organs; and in sugar-sensing neurons of a small number of taste bristles in the tarsus.

      (FlyBase Curators, 2017)

      ScerGAL4Gr43a-GAL4 drives expression in around four cells of the larval pharyngeal sense organ projecting to the embryonic/larval subesophageal ganglion.

      (Mishra et al., 2013)

      ScerGAL4Gr43a-GAL4 drives strong expression in two S cells in the middle segment of the prothoracic tarsal segment 5 and weak expression in two S cells at the tip of this segment. A total of 4 neurons are labelled in the adult proventriculus, 2 extend axons to the subesophageal ganglion and 2 to muscles of the mid gut, all 4 extend dentrites in the foregut lumen.

      (Miyamoto et al., 2012)
       
      Marker for
      Reflects expression of
      Gr43a
      (Mishra et al., 2013, Miyamoto et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr43a-GAL4 cells in adults does not significantly affect preference for sucrose, compared to controls.

      (Thoma et al., 2016)

      Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.UAS.Citrine with orange light) of the combined set of Scer\GAL4Gr28b.b.4.7 and Scer\GAL4Gr43a-GAL4 cells, but not Scer\GAL4Gr43a-GAL4 cells alone, in adults significantly increases food intake of satiated flies, compared to controls, but this is to a significantly reduced extent compared to Scer\GAL4Gr28b.b.4.7 cells alone.

      (Zhan et al., 2016)

      Inhibition (using shits1.IVS.p10.20xUAS at 33[o]C) of a subset of Scer\GAL4Gr43a-GAL4 cells (restricted using Scer\GAL80ChAT.7.4P) in adults during training significantly reduces long-term, but not short-term, associative appetitive (sugar) olfactory memory, compared to controls.

      (Yamagata et al., 2015)

      Larvae in which neural transmission has been blocked in the Scer\GAL4Gr43a-GAL4-expressing cells in the brain show a normal preference for fructose but do not show the usual preference for glucose and sorbitol.

      (Mishra et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (21)