Open Close
General Information
Symbol
Dmel\Cdk1T14A.Y15F.UASp.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0294235
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Cdk1AF
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp sequences regulate expression of Cdk1 carrying T14A and Y15F substitutions (which prevents inhibitory phosphorylation by Wee1 and Myt1 kinases on these residues), and tagged at the C terminus with Venus.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Cdk1T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4bam.T:Hsim\VP16 leads to partial male sterility.

Adult-specific expression of Cdk1T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus (under the control of RU486-dependent Scer\GAL4elav.Switch.PO) results in a significant reduction in sleep.

Expression of Cdk1T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus in the pars lateralis under the control of Scer\GAL4Crz.504.Δ311-249 results in a significant reduction in sleep.

Expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus via Scer\GAL4sd.PU results in wing margin defects. Wing discs are smaller than normal and abnormally shaped.

Expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus via Scer\GAL4ey.PU results in eyeless, pharates that are unable to eclose.

Scer\GAL4en.PU-mediated expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus increases apoptosis and the mitotic index in the posterior of the wing imaginal disc. Wing discs are smaller than normal and abnormally shaped.

Scer\GAL4neur-P72-mediated expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus in the sensory organ precursor cells of the zone of non-proliferating cells of the wing disc initiates mitosis in these cells, which are normally arrested in G2 phase.

One hour after exposure to 40-Gy ionizing radiation, Scer\GAL4en.PU cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus wing discs show mitotic cells in the posterior compartment, indicating a lack of normal checkpoint response.

Scer\GAL4pros.PU-mediated expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus in type I larval brain neuroblasts results in a relatively low mitotic index (after treatment with colchicine). Almost half of these neuroblasts exhibit gross chromosome aberrations. Mitosis takes relatively longer in these cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of CycBScer\UAS.T:Ivir\HA1 enhances the wing phenotype of Scer\GAL4sd.PU cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus flies, resulting in complete loss of the wing.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Scer\GAL4tub.PU-mediated expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus does not rescue the pupal lethality of cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Cdk1T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus
Cdk1T14A.Y15F.UASp.Venus
cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus
Name Synonyms
Secondary FlyBase IDs
    References (4)