Open Close
General Information
Symbol
Dmel\Cdk1B47
Species
D. melanogaster
Name
FlyBase ID
FBal0030731
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
cdc2B47, Dmcdc2B47
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

T10386027C

Reported nucleotide change:

T?C

Comment:

Mutation of the intron 1 splice donor site from GT to GC.

Comment:

destroys splice consensus at 5' end of first intron

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation destroys a splice consensus sequence at the 5' end of the first intron.

Mutation of the GT intron 1 splice donor site. Nucleotide substitution: GT to GC.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C die during the pupal stage.

At the restrictive temperature of 29[o]C, cdc2E1-24/cdc2B47 oocytes show prolonged prophase I at stage 13 and abnormal DNA morphology at stage 14.

In cdc2B47 mutants, the NB6-4t lineage develops as in wild-type, generating two glial cells and five-to-six dorso-lateral neurons.

When cdc2B47/cdc2E1-24 animals are raiseds at a semi-restrictive temperature (26oC), flies develop until the end of pupariation, but fail to hatch. These animals exhibit a severe loss of bristles. In addition, at 26h APF about 50% of sensory organs are composed of only two cells, lacking shaft and socket cells.

Lethal phase is during the larval/pupal stage. Homozygous embryos develop essentially normally. RP2 neuron duplication and large RP2 neurons are seen in only 3.6% and 3% of cases respectively. Duplication of muscle DA1 can be seen in embryos carrying four copies of cdc2A171T (in a cdc2B47 background) which have been shifted to the non-permissive temperature. RP2 neuron duplication and large RP2 neurons are seen in 42.7% and 4.6% of cases respectively. Temperature upshifts can also result in the arrest of neuroblasts in G2 in these embryos.

Somatic clones in the wing lead to wing cells that are larger than normal which produce either longer than normal wing hairs, or double wing hairs.

cdc2B47/cdc2E1-24 larvae at the restrictive temperature show mitotic defects resulting in a reduction in the size of the imaginal discs and a reduction in the cell density in the optic lobe. The nuclei of the abdominal histoblasts are enlarged, and the DNA content is increased to about 16C.

cdc2B47/cdc2E1-24 females cease to lay eggs when shifted to the restrictive temperature of 29oC. Dividing cells in the ovary disappear over a period of 5 days after shifting to 29oC, while nurse cell nuclei persist and increase in size.

The cdc2B47/cdc2E1-24 combination is temperature sensitive. cdc2B47/cdc2E1-24 third instar mutants the size of the central nervous system and imaginal discs is severly reduced. Many of the mutants cells in the CNS, salivary gland and imaginal discs are larger and more intensely DAPI-staining than wild type. Cell division is reduced but BrdU incorporation occurs in the salivary gland imaginal ring and CNS during the third instar. Mutations at esg have no further effect on these mutant phenotypes. In cdc2B47/Df(2L)J27, many cells of the outer part of the optic lobe contain huge bnanded polytene chromsomes comparable in size and morphology to the salivary gland polytene chromosomes. Abdominal histoblasts in cdc2B47/cdc2E1-24 larvae accumulate BrdU without increasing cell number, suggesting they are in endocycle.

Males carrying two or four copies of P{Dmcdc2A171T} at 18oC or 29oC exhibit a reduction in testis cell number and increase in size of the nucleus and Nebenkern. Results indicate only one meiotic division occurs in males with two copies of P{Dmcdc2A171T} and no meiotic divisions in males with four copies. Males carrying two copies still exhibit spermatid differentiation.

Larvae contain no imaginal discs. Brain development is abnormal.

Die after pupariation at the larval-pupal interface. Larvae take 1-2 days longer than wild type to reach the wandering stage. Imaginal discs are missing or rudimentary and larval brains are reduced in size. Endoreplication and polytenization of larval cells is normal, as are salivary gland size and intensity of DNA staining of their polytene nuclei.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

cdc2[+]/Cdk1B47 is a suppressor of increased cell death phenotype of shtd1

Cdk1B47 is a suppressor of hyperplasia phenotype of wtse26-1

Cdk1B47 is a suppressor of hyperplasia phenotype of wtsP8

Cdk1B47 is a suppressor of abnormal size | adult stage phenotype of wtsP8

Other
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

cdc2[+]/Cdk1B47 is a non-enhancer of eye | somatic clone phenotype of gfr1

cdc2[+]/Cdk1B47 is a non-enhancer of phenotype of pngunspecified

Suppressor of
Statement
Reference

cdc2[+]/Cdk1B47 is a suppressor of eye phenotype of shtd1

cdc2[+]/Cdk1B47 is a suppressor of ommatidium phenotype of shtd1

Cdk1B47 is a suppressor of eye phenotype of wtsP8

Cdk1B47 is a suppressor of pupa phenotype of wtse26-1

NOT Suppressor of
Statement
Reference

cdc2[+]/Cdk1B47 is a non-suppressor of eye | somatic clone phenotype of gfr1

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr1 is not modified by cdc2B47/+.

The defects in the abdominal histoblasts are partially rescued by CycAScer\UAS.cHa expressed under the control of Scer\GAL4P127; the increase in DNA content is limited to 8C.

Dominantly suppresses the pupal lethality and overproliferation phenotype of wtsP8 homozygotes. The disc overproliferation phenotype of wtse26-1 homozygotes is suppressed by one copy of cdc2B47.

In cdc2B47/+,esg35Ce-3/esg05729 imaginal discs are reduced in size and sometimes have cells with large nuclei.

Xenogenetic Interactions
Statement
Reference

cdc2B47 enhances the eye phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4dpp.PH.

The defects of cdc2B47/cdc2E1-24 larvae are rescued by Xlae\cdc2Scer\UAS.cHa expressed under the control of Scer\GAL4P127, but not by Xlae\cdc2K33R.Scer\UAS expressed under the control of Scer\GAL4P127. Coexpression of CycAScer\UAS.cHa and Xlae\cdc2K33R.Scer\UAS under the control of Scer\GAL4P127 completely rescues the abdominal histoblast defects seen in cdc2B47/cdc2E1-24 larvae at the restrictive temperature. The imaginal disc defects seen in these larvae are not rescued.

Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments

Scer\GAL4tub.PU-mediated expression of cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus rescues the pupal lethality of cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4tub.PU-mediated expression of cdc2T14A.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus rescues the pupal lethality of cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4tub.PU-mediated expression of cdc2Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus does not rescue the pupal lethality of cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4tub.PU-mediated expression of cdc2T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus does not rescue the pupal lethality of cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C.

Lethality of heterozygotes with Df(2L)J27 can be partially rescued by expression of cdc2A171T, cdc2G43E or cdc2P131S at 18oC, levels of rescue drop at higher temperatures.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (27)