FlyBase Allele Report: Dmel\Sdhaf3[null]

General Information

Symbol

Dmel\Sdhaf3^null

Species

D. melanogaster

Name

FlyBase ID

FBal0298308

Feature type

allele

Associated gene

Dmel\Sdhaf3

Associated Insertion(s)

TI{TI}Sdhaf3^null

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Na et al., 2014)

Mutagen

gene targeting by homologous recombination

(Na et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}Sdhaf3^null

Cytology

Description

339bp deletion in the Sdhaf3 coding region, removing exons 1 and 2. A loxP cassette containing the w^+mW.hs marker is inserted in place of the deleted sequence.

(Na et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | progressive

(Na et al., 2014)

abnormal oxidative stress response

(Na et al., 2014)

chemical sensitive

(Na et al., 2014)

fertile

(Na et al., 2014)

short lived | chemical conditional

(Na et al., 2014)

viable

(Na et al., 2014)

Phenotype Manifest In

wing | conditional

(Na et al., 2014)

Detailed Description

Statement

Reference

Mutants progress normally through development and have a normal lifespan when maintained on standard growth media. However, these animals are sensitive to ethanol and oxidative stress, resulting from either paraquat treatment or hyperoxia - mutants show a 50% reduction in lifespan relative to controls in response to hyperoxia and hold their wings erect (indicative of mitochondrial dysfunction and muscle degeneration).

Although fully viable and fertile, Sdhaf3^null flies display an age-dependent reduction in motility.

(Na et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sdhaf3^null has abnormal oxidative stress response phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Sod2^UAS.cUa

(Na et al., 2014)

NOT suppressed by

Statement

Reference

Sdhaf3^null has abnormal oxidative stress response phenotype, non-suppressible by CG34229^UAS.cNa/Scer\GAL4^Act5C.PI

(Na et al., 2014)

Other

Statement

Reference

SdhB^EY12081, Sdhaf3^null has partially lethal phenotype

(Na et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sdhaf3^null is rescued by Scer\GAL4^Act5C.PI/Sdhaf3^UAS.cNa

(Na et al., 2014)

Partially rescued by

Sdhaf3^null is partially rescued by Sdhaf3^UAS.cNa/Scer\GAL4^C57

(Na et al., 2014)

Sdhaf3^null is partially rescued by Scer\GAL4^elav.PU/Sdhaf3^UAS.cNa

(Na et al., 2014)

Not rescued by

Sdhaf3^null is not rescued by Scer\GAL4^Cg.PA/Sdhaf3^UAS.cNa

(Na et al., 2014)

Sdhaf3^null is not rescued by Scer\GAL4^mex1.2.1/Sdhaf3^UAS.cNa

(Na et al., 2014)

Comments

Scer\GAL4^Act5C.PI-mediated expression of Sdhaf3^Scer\UAS.cNa rescues the hyperoxia sensitivity and climbing defects of Sdhaf3^null mutants, while Scer\GAL4^C57- or Scer\GAL4^elav.PU-mediated expression provides partial rescue.

(Na et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76575

w¹¹¹⁸; TI{TI}Sdhaf3^null

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sdhaf3^null

dSdhaf3^-

(Na et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close