FB2026_02 , released June 18, 2026
Allele: Scer\GAL4ChAT-MI04508-TG4.0
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General Information
Symbol
Scer\GAL4ChAT-MI04508-TG4.0
Species
S. cerevisiae
Name
FlyBase ID
FBal0304241
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Mi{Trojan-GAL4.0}ChATMI04508-TG4.0
Carried in Construct
Also Known As
ChAT-GAL4
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Diao et al., 2015)
Progenitor genotype
Mi{MIC}ChATMI04508
(Diao et al., 2015)
Associated Insertion(s)
Mi{Trojan-GAL4.0}ChATMI04508-TG4.0
Cytology
Description

The attP cassette in the Mi{MIC}ChATMI04508 insertion (which is in a coding intron common to all splice forms of ChAT) has been replaced with a Trojan GAL4 cassette that contains a splice acceptor site followed by the T2A peptide, the Scer\GAL4 coding sequence and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap ChAT. The splice acceptor ensures that the T2A-Scer\GAL4 open reading frame is incorporated into the ChAT mRNA, while the T2A sequence is expected to truncate the native ChAT gene product and promotes the separate translation of the Scer\GAL4 open reading frame. Thus Scer\GAL4 expression is driven by the native ChAT regulatory sequences.

(Diao et al., 2015)
Allele components
Component
Use(s)
Inserted element
Mi{Trojan-GAL4.0}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      cholinergic neuron
      (Diao et al., 2015)
      sensory neuron
      (Diao et al., 2015)
      larval DA1 motor neuron
      (Diao et al., 2015)
      pCC neuron
      (Diao et al., 2015)
      adult stage
      cholinergic neuron
      (Lacin et al., 2019)
      lobula columnar neuron LC11
      (Keleş et al., 2020)
      medulla intrinsic neuron Mi1
      (Davis et al., 2020)
      Additional Information
      Statement
      Reference

      ScerGAL4ChAT-MI04508-TG4.0 drives expression in neurons in the adult thoracico-abdominal ganglion that express ChAT; it also drives expression in neurons labeled by GABA or VGlut1 antibodies.

      (Lacin et al., 2019)

      ScerGAL4ChAT-MI04508-TG4.0 drives expression in neurons that use acetylcholine as a neurotransmitter, and replicates the ChAT immunoreactivity pattern. Expression is observed in most neurons of the larval CNS, and in sensory neurons of the larval PNS.

      (Diao et al., 2015)
       
      Marker for
      Reflects expression of
      ChAT
      (Lacin et al., 2019, Bloomington Drosophila Stock Center, 2017, Diao et al., 2015)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ChATGAL4
      (Lacin et al., 2019)
      Scer\GAL4ChAT-MI04508-TG4.0
      Name Synonyms
      Secondary FlyBase IDs
        References (17)