FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\Mi{Trojan-GAL4.0}ChATMI04508-TG4.0
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General Information
Symbol
Dmel\Mi{Trojan-GAL4.0}ChATMI04508-TG4.0
Species
D. melanogaster
Name
FlyBase ID
FBti0168134
Feature type
transposable_element_insertion_site
Also Known As
ChAT-GAL4
Description
Inserted Element
Mi{Trojan-GAL4.0}
LINE ID
Affected gene(s)
CG7715, ChAT
Viability / fertility
Associated allele(s)
CG7715MI04508-TG4.0-X, ChATMI04508-TG4.0, Scer\GAL4ChAT-MI04508-TG4.0
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3R ( 91C4 )
Sequence Location
Target / Docking site
Mi{MIC}ChATMI04508
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
91C4 (near gene of known cytology)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Mi{Trojan-GAL4.0}
(Diao et al., 2015)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
CG7715
(FlyBase, 1996-)
ChAT
(Diao et al., 2024, Keleş et al., 2020, Diao et al., 2015)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
cholinergic neuron
(Diao et al., 2015)
sensory neuron
(Diao et al., 2015)
larval DA1 motor neuron
(Diao et al., 2015)
pCC neuron
(Diao et al., 2015)
adult stage
cholinergic neuron
(Lacin et al., 2019)
lobula columnar neuron LC11
(Keleş et al., 2020)
medulla intrinsic neuron Mi1
(Davis et al., 2020)
Additional Information
Statement
Reference

ScerGAL4ChAT-MI04508-TG4.0 drives expression in neurons in the adult thoracico-abdominal ganglion that express ChAT; it also drives expression in neurons labeled by GABA or VGlut1 antibodies.

(Lacin et al., 2019)

ScerGAL4ChAT-MI04508-TG4.0 drives expression in neurons that use acetylcholine as a neurotransmitter, and replicates the ChAT immunoreactivity pattern. Expression is observed in most neurons of the larval CNS, and in sensory neurons of the larval PNS.

(Diao et al., 2015)
 
Marker for
Reflects expression of
ChAT
(Lacin et al., 2019, Bloomington Drosophila Stock Center, 2017, Diao et al., 2015)
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Replacement descendant of (target)
Mi{MIC}ChATMI04508
(Diao et al., 2015)
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 0 "Trojan GAL4" cassette from the pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70 plasmid.

(Diao et al., 2015)
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
ChAT-GAL4
(Delandre et al., 2025, Silva et al., 2025, Yu et al., 2025, Chen et al., 2023, Lacin et al., 2019, Watanabe et al., 2017)
ChAT-GAL4 MI04508
(Estacio-Gómez et al., 2020)
ChAT-Gal4
(Chvilicek et al., 2025, Katheder et al., 2023)
ChAT-T2A-Gal4
(King et al., 2020)
ChATMI-GAL4
(Ho et al., 2022)
Cha-Gal4
(Raouf Issa et al., 2022)
ChaMI04508-Gal4
(Diao et al., 2015)
ChaTMI04508-GAL4
(Ho et al., 2022)
ChaTTE-Gal4
(Diao et al., 2024)
Mi{Trojan-GAL4.0}ChATMI04508-TG4.0
(Katheder et al., 2023, Keleş et al., 2020)
VAChT-GAL4
(Chen et al., 2023)
Name Synonyms
Secondary FlyBase IDs
    References (17)