FlyBase Allele Report: Scer\GAL4[DBD.R32D11]

General Information

Symbol

Scer\GAL4^DBD.R32D11

Species

S. cerevisiae

Name

FlyBase ID

FBal0304452

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R32D11-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R32D11-GAL4.DBD}

Cytology

Description

The R32D11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR32D11

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PAM neuron 10 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 11 | intense

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 13 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 14 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)

mushroom body alpha lobe slice 1

Comment: when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

(Aso and Rubin, 2016)

dopaminergic PAM neuron 11

Comment: when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

(Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R58E02

(Takemura et al., 2017)

abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R58E02

(Takemura et al., 2017)

abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1^{Chrimson.IVS.Syn21.10xUAS.tdTomato}, Hsap\RELA^AD.R58E02

(Takemura et al., 2017)

Phenotype Manifest In

mushroom body output neuron 7 | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R58E02

(Takemura et al., 2017)

mushroom body output neuron 7 | conditional, with Cnoc\ChR1^{Chrimson.IVS.Syn21.10xUAS.tdTomato}, Hsap\RELA^AD.R58E02

(Takemura et al., 2017)

Detailed Description

Statement

Reference

Optogenetic activation (using Zzzz\ChR^{Chrimson.IVS.Syn21.10xUAS.tdTomato} or Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R32D11 and Hsap\RELA^AD.R58E02 in adult females results in a strong, slow depolarization of MBON-alpha1 (mushroom body output neuron 7), sufficient to elicit a spiking response.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R32D11 and Hsap\RELA^AD.R58E02 in adult females, in combination with a paired odor, results in a short-term associative appetitive olfactory memory for this odor; preference is significantly greater when these neurons are not also activated during the testing period.

(Takemura et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions