FlyBase Allele Report: Dmel\Stam[19]

General Information

Symbol

Dmel\Stam¹⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0319593

Feature type

allele

Associated gene

Dmel\Stam

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Sheng et al., 2016)

Progenitor genotype

Cytology

Description

Amino acid replacement: W269R.

(Sheng et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,860,190..10,860,190 [+]

Nucleotide change:

T10860190A

Reported nucleotide change:

T?A

Amino acid change:

W269R | Stam-PA

Reported amino acid change:

W269R

(Sheng et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | somatic clone | third instar larval stage

(Sheng et al., 2016)

Phenotype Manifest In

endosome | somatic clone | third instar larval stage

(Sheng et al., 2016)

eye disc | somatic clone | third instar larval stage

(Sheng et al., 2016)

photoreceptor neuron | somatic clone | third instar larval stage

(Sheng et al., 2016)

wing disc | somatic clone | third instar larval stage

(Sheng et al., 2016)

Detailed Description

Statement

Reference

Somatic clones of Stam¹⁹/Stam¹⁹ cells in the third instar larval eye or wing disc display low levels of apoptosis (detected by Caspase-3 staining) and show abnormal localization of rho in endosomes, which often display both early and late markers.

Differentiation of the R8 photoreceptor in Stam¹⁹/Stam¹⁹ somatic clones in the third instar larval eye disc is normal but differentiation of other photoreceptors is slightly delayed.

(Sheng et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Stam¹⁹ has increased cell death | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ/Rbf^15aΔ

(Sheng et al., 2016)

Suppressed by

Statement

Reference

Rbf^15aΔ, Stam¹⁹ has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by hid^w138

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by E2f1ⁱ²/E2f1^rM729

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^Act.PU

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Egfr^2.A887T.UAS

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by Scer\GAL4^Act.PU/spi^s.UAS

(Sheng et al., 2016)

NOT suppressed by

Statement

Reference

Rbf^15aΔ, Stam¹⁹ has increased cell death | somatic clone | third instar larval stage phenotype, non-suppressible by rho^UAS.cdCa/Scer\GAL4^Act.PU

(Sheng et al., 2016)

Enhancer of

Statement

Reference

Stam¹⁹/Stam¹⁹ is an enhancer of increased cell death | third instar larval stage | somatic clone phenotype of Rbf^15aΔ

(Sheng et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Stam¹⁹ has eye disc | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ/Rbf^15aΔ

(Sheng et al., 2016)

Stam¹⁹ has wing disc | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ/Rbf^15aΔ

(Sheng et al., 2016)

Suppressed by

Statement

Reference

Rbf^15aΔ, Stam¹⁹ has eye disc | somatic clone | third instar larval stage phenotype, suppressible by E2f1ⁱ²/E2f1^rM729

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has eye disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Ras85D^V12.UAS/Scer\GAL4^Act.PU

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has eye disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Egfr^2.A887T.UAS

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has eye disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/spi^s.UAS

(Sheng et al., 2016)

Rbf^15aΔ, Stam¹⁹ has eye disc | somatic clone | third instar larval stage phenotype, suppressible by hid^w138

(Sheng et al., 2016)

NOT suppressed by

Statement

Reference

Rbf^15aΔ, Stam¹⁹ has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by rho^UAS.cdCa/Scer\GAL4^Act.PU

(Sheng et al., 2016)

Enhancer of

Statement

Reference

Stam¹⁹/Stam¹⁹ is an enhancer of eye disc | third instar larval stage | somatic clone phenotype of Rbf^15aΔ

(Sheng et al., 2016)

Stam¹⁹/Stam¹⁹ is an enhancer of wing disc | third instar larval stage | somatic clone phenotype of Rbf^15aΔ

(Sheng et al., 2016)

Other

Statement

Reference

Rbf^15aΔ, Stam¹⁹ has eye | somatic clone | adult stage phenotype

(Sheng et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Somatic clones of Rbf^15aΔ;Stam¹⁹ double homozygous cells display significantly higher levels of apoptosis in third instar larval wing and eye discs, even in clones posterior to the eye disc morphogenetic furrow, compared to clones homozygous for either Rbf^15aΔ or Stam¹⁹ alone. In the adult eye, the double homozygous clones are very small and difficult to recover compared to each of the single homozygous clones.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic in third instar larval eye disc is completely abolished when the clones are created in hid^w138 mutant background.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic clones in third instar larval eye disc is completely abolished when the clones are created in E2f1[i2]/E2f1[rM729] mutant background.

(Sheng et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Stam^2L2896

(Sheng et al., 2016)

Stam^2L3297

(Sheng et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Stam¹⁹

stam¹⁹

(Sheng et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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