FlyBase Allele Report: Dmel\Vps8[1]

General Information

Symbol

Dmel\Vps8¹

Species

D. melanogaster

Name

FlyBase ID

FBal0320420

Feature type

allele

Associated gene

Dmel\Vps8

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Lőrincz et al., 2016)

Progenitor genotype

Cytology

Description

4bp deletion (nucleotides 186-189) and 12bp deletion (nucleotides 419-431), both within Vps8. The 4bp deletion results in a frameshift and a premature stop codon which is predicted to result in the translation of only the first 39 amino acid residues of Vps8.

(Lőrincz et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:6,495,002..6,495,005 [-]

Comment:

A 4 bp deletion causes a frameshift and immediate stop codon after the first 39 amino acids of Vps8. There is also a downstream 12 bp deletion in Vps8 ( 3L:6494760..6494771 ) in the mutant strain.

(Lőrincz et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | third instar larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

increased cell size (with Df(3L)ED211)

(Lőrincz et al., 2019)

majority die during pharate adult stage

(Lőrincz et al., 2016)

majority die during pharate adult stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

melanotic mass phenotype | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

visible (with Df(3L)ED211)

(Lőrincz et al., 2016)

Phenotype Manifest In

embryonic/larval crystal cell | larval stage | increased number (with Df(3L)ED211)

(Lőrincz et al., 2016)

embryonic/larval garland cell | larval stage

(Lőrincz et al., 2016)

embryonic/larval garland cell | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

embryonic/larval hemocyte | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

embryonic/larval hemocyte | larval stage | increased number (with Df(3L)ED211)

(Lőrincz et al., 2016)

endolysosome | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

garland cell (with Df(3L)ED211)

(Lőrincz et al., 2019)

lamellocyte | larval stage | increased number (with Df(3L)ED211)

(Lőrincz et al., 2016)

larval sessile hemocyte | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

late endosome (with Df(3L)ED211)

(Lőrincz et al., 2019)

late endosome | larval stage

(Lőrincz et al., 2016)

late endosome | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

vacuole | larval stage (with Df(3L)ED211)

(Lőrincz et al., 2016)

wing (with Df(3L)ED211)

(Lőrincz et al., 2016)

Detailed Description

Statement

Reference

Vps8¹/Df(3L)ED211 transheterozygous garland cells are enlarged and show smaller late endosomes (Rab7-positive puncta) than controls, but do not show an obvious increase in the number of autophagosomes (endogenous Atg8a-positive puncta).

(Lőrincz et al., 2019)

Vps8¹/Vps8¹ and Vps8¹/Df(3L)ED211 mutant flies are semilethal, with most dying as pharate adults. Dissected nephrocytes of Vps8¹/Df(3L)ED211 and Vps8¹/Vps8¹ larvae display defective endosomal trafficking as shown by incubation with, and subsequent analysis of internalization and transport of fluorescent dextran. Garland nephrocytes in these mutants also exhibit late endosome and endolysosome fragmentation, and absent α-vacuoles, but early endosomes are unaffected.

Surviving Vps8¹/Df(3L)ED211 adults are weak, fail to unfold their wings properly, and die within 24 hr, but do not display any eye pigmentation defects. Vps8¹/Df(3L)ED211 mutant larvae have significantly more circulating hematocytes than controls, and often contain hemocyte-derived melanotic tumors in their body cavity, disorganization of the sessile hemocyte compartment, and accumulation of crystal cells and lamellocytes. Hemocytes of these mutants fail to acidify bacteria-containing phagosomes upon injection of E. coli. In an assay of nephrocyte function using food supplementation with silver nitrate, Vps8¹/Df(3L)ED211 larvae exhibit impaired silver uptake and endo-lysosomal trafficking. The endosomal compartment of Vps8¹/Df(3L)ED211 mutant wing imaginal discs is indistinguishable from wild type.

(Lőrincz et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Vps8¹/Df(3L)ED211 has melanotic mass phenotype | larval stage phenotype, non-suppressible by Df(2L)lt45/lt^LL07138

(Lőrincz et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Vps8¹/Df(3L)ED211 has embryonic/larval garland cell | larval stage phenotype, enhanceable by Df(2L)lt45/lt^LL07138

(Lőrincz et al., 2016)

NOT suppressed by

Statement

Reference

Vps8¹/Df(3L)ED211 has late endosome | larval stage phenotype, non-suppressible by Df(2L)lt45/lt^LL07138

(Lőrincz et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

lt^LL07138/Df(2L)lt45, Vps8¹/Df(3L)ED211 double mutants exhibit similar melanotic tumor-formation and nephrocyte late endosome fragmentation phenotypes as compared to Vps8¹/Df(3L)ED211 single mutants, and nephrocytes also exhibit many small electron dense lysosome-like organelles.

(Lőrincz et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vps8¹ is rescued by Vps8^Tag:HA

(Lőrincz et al., 2016)

Vps8¹/Df(3L)ED211 is rescued by Vps8^Tag:HA

(Lőrincz et al., 2016)

Not rescued by

Vps8¹/Df(3L)ED211 is not rescued by Vps8^UAS.cLa/Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

Comments

Expression of Vps8^T:Ivir\HA1 rescues the semilethality, nephrocyte enlargement and function defects, late endosome and endolysosome fragmentation, and α-vacuole absence of Vps8¹/Vps8¹ and Vps8¹/Df(3L)ED211 mutant flies, and rescues the increased hemocyte number, melanotic tumor formation, and defective bacteria-containing endosome trafficking of Vps8¹/Df(3L)ED211 mutant flies.

(Lőrincz et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Vps8¹

vps8¹

(Lőrincz et al., 2019, Lőrincz et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

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