FlyBase Allele Report: Dmel\Pld[3.1]

General Information

Symbol

Dmel\Pld^3.1

Species

D. melanogaster

Name

FlyBase ID

FBal0324251

Feature type

allele

Associated gene

Dmel\Pld

Associated Insertion(s)

TI{TI}Pld^3.1

Carried in Construct

Also Known As

dPLD^3.1

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Thakur et al., 2016)

Mutagen

gene targeting by homologous recombination

(Thakur et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{TI}Pld^3.1

Cytology

Description

The Pld HKD1 motif has been disrupted by the insertion of sequence containing a w^+* marker and stop codons in all three frames on both strands.

(Thakur et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | progressive

(Panda et al., 2018)

Phenotype Manifest In

eye photoreceptor cell | progressive

(Panda et al., 2018)

ommatidium | adult stage | progressive | conditional

(Thakur et al., 2016)

retina | adult stage | progressive | conditional

(Thakur et al., 2016)

retina | adult stage | progressive | conditional (with Df(2R)ED1612)

(Thakur et al., 2016)

retina | adult stage | progressive | conditional (with Pld^3.3)

(Thakur et al., 2016)

Detailed Description

Statement

Reference

Pld^3.1 adults exhibit retinal degeneration (normal numbered eye photoreceptor cells on the day of eclosion followed by a progressively decreased number of eye photoreceptor cells) when compared to controls.

(Panda et al., 2018)

Pld^3.1 flies are homozygous viable and at eclosion their photoreceptor ultrastructure is indistinguishable from wild-type. Upon illumination however, the reduction in the volume fraction of the cell occupied by the rhabdomere in peripheral photoreceptors R1-R6 is more pronounced than in wild-type and Pld^3.1 mutants also display enhanced accumulation of rhodopsin1-containing vesicles (RLVs) in a Rab7 positive compartment. The mutant photoreceptors are less sensitive to light compared to eye color-matched controls at eclosion but do not exhibit defects in electrical response.

Under constant illumination the Pld^3.1 mutants display progressive light-dependent retinal degeneration, characterized by a collapse of the apical microvillar membrane and accumulation of membranous whorls within the cell body. Light-induced retinal degeneration is also observed in Pld^3.1/Pld^3.3 or Pld^3.1/Df(2R)ED1612 transheterozygous flies but not in flies heterozygous for either Pld^3.1 or Pld^3.3.

(Thakur et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pld^3.1 has decreased cell number | adult stage | progressive phenotype, suppressible by Scer\GAL4^hs.PU/Hsap\PLD1^UAS.cPa

(Panda et al., 2018)

Pld^3.1 has decreased cell number | adult stage | progressive phenotype, suppressible | partially by Hsap\PLD2^UAS.cPa/Scer\GAL4^hs.PU

(Panda et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pld^3.1 has eye photoreceptor cell | progressive phenotype, suppressible by Scer\GAL4^hs.PU/Hsap\PLD1^UAS.cPa

(Panda et al., 2018)

Pld^3.1 has eye photoreceptor cell | progressive phenotype, suppressible | partially by Hsap\PLD2^UAS.cPa/Scer\GAL4^hs.PU

(Panda et al., 2018)

Pld^3.1 has retina | adult stage | progressive | conditional phenotype, suppressible | partially by laza²²/laza²²

(Thakur et al., 2016)

Pld^3.1 has ommatidium | adult stage | progressive | conditional phenotype, suppressible | partially by laza²²/laza²²

(Thakur et al., 2016)

Pld^3.1 has retina | adult stage | progressive | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/rdgA^UAS.cRa

(Thakur et al., 2016)

Pld^3.1 has ommatidium | adult stage | progressive | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/rdgA^UAS.cRa

(Thakur et al., 2016)

Pld^3.1 has retina | adult stage | progressive | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Vps35^UAS.Tag:HA

(Thakur et al., 2016)

Pld^3.1 has ommatidium | adult stage | progressive | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Vps35^UAS.Tag:HA

(Thakur et al., 2016)

Pld^3.1 has retina | adult stage | progressive | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/garz^UAS.cUa

(Thakur et al., 2016)

Pld^3.1 has ommatidium | adult stage | progressive | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/garz^UAS.cUa

(Thakur et al., 2016)

Suppressor of

Statement

Reference

Pld^3.1/Pld^3.1 is a suppressor of ommatidium | adult stage | conditional phenotype of shi¹

(Thakur et al., 2016)

Pld^3.1/Pld^3.1 is a suppressor of ommatidium | adult stage phenotype of Arf1^CA.UAS.cUa, Scer\GAL4^ninaE.PU

(Thakur et al., 2016)

Pld^3.1/Pld^3.1 is a suppressor of rhabdomere | adult stage phenotype of Arf1^CA.UAS.cUa, Scer\GAL4^ninaE.PU

(Thakur et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The light-dependent retinal degeneration observed in Pld^3.1 homozygous adults is suppressed to a varying degree by combination with laza²² (in homozygous state) or by expression of any of the following: rdgA^Scer\UAS.cRa, Vps35^{Scer\UAS.T:Ivir\HA1} or garz^Scer\UAS.cUa under the control of Scer\GAL4^ninaE.PU in the mutant background.

The ultrastructural defects in the rhabdomere characteristic for newly eclosed adult flies expressing Arf79F^{CA.Scer\UAS.cUa} under the control of Scer\GAL4^ninaE.PU are rescued by combination with the Pld^3.1 allele in homozygous state.

The abnormal drop in the number of rhodopsin1-containing vesicles (RLVs) observed upon a pulse of bright white light administered to shi¹ homozygous adult flies, that have been kept at the permissive temperature of 18[o]C, and followed by a rapid transfer to the non-permissive temperature (25[o]C) is not observed when the shi¹ mutant allele is combined with Pld^3.1 in homozygous state.

(Thakur et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pld^3.1 is rescued by Pld^UAS.cRa/Scer\GAL4^hs.PU

(Panda et al., 2018)

Pld^3.1 is rescued by Pld^UAS.cRa/Scer\GAL4^hs.PU

(Thakur et al., 2016)

Not rescued by

Pld^3.1 is not rescued by Scer\GAL4^hs.PU/Pld^K1086R.UAS

(Thakur et al., 2016)

Comments

The light-dependent retinal regeneration observed in Pld^3.1 homozygous adults is rescued by expression of Pld^Scer\UAS.cRa but not Pld^{K1086R.Scer\UAS} under the control of Scer\GAL4^hs.PU in the mutant background.

(Thakur et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pld^3.1

dPLD^3.1

(Panda et al., 2018, Thakur et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

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