FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PldUAS.cRa
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General Information
Symbol
Dmel\PldUAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Raghu
FlyBase ID
FBal0241089
Feature type
allele
Associated gene
Dmel\Pld
Associated Insertion(s)
Carried in Construct
P{UAS-Pld.R}
Key Links
Transgenic product class
wild_type
(Raghu et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Raghu et al., 2009)
Mutagen
in vitro construct
(Raghu et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Pld.R}
Cytology
Description

UASt regulatory sequences drive expression of the Pld open reading frame.

(Raghu et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Pld
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing PldUAS.cRa under the control of Scer\GAL4VGlut-OK371 leads to larval NMJ showing fewer boutons than controls.

      (Kankel et al., 2020)

      Expression of PldUAS.cRa under the control of Scer\GAL4ninaE.PU leads to retinal degeneration (normal numbered eye photoreceptor cells on the day of eclosion followed by a progressively decreased number of eye photoreceptor cells) when compared to controls.

      (Panda et al., 2018)

      Expression of PldScer\UAS.cRa under the control of Scer\GAL4ninaE.PU results in a decreased number of rhodopsin1-containing vesicles (RLVs) per ommatidium upon illumination in adult flies, in contrast to wild-type where the RLV number slightly increases compared to pre-illumination.

      (Thakur et al., 2016)

      Flies expressing PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF show vesiculation and degeneration of the rhabdomeres. In addition, the cell body of the photoreceptors shows accumulation of expanded tubulovesicular membraneous structures, which appear as concentric stacks of membranes in the most severe cases.

      Retinal extracts from flies expressing PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF show a significant elevation of a single species of phosphatidic acid (predicted fatty acyl composition 16:0 / 18:2 ) compared to controls. The levels of phosphatidylcholine and phosphatidylinositol in the retinal extracts are normal.

      (Raghu et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expressing TBPHN493D.UAS and PldUAS.cRa under the control of Scer\GAL4VGlut-OK371 leads to extensive larval NMJ disorganization.

      (Kankel et al., 2020)

      The abnormal drop in the number of rhodopsin1-containing vesicles per ommatidium upon illumination observed in adult flies expressing PldScer\UAS.cRa under the control of Scer\GAL4ninaE.PU is fully suppressed by co-expression of either Vps35dsRNA.Scer\UAS.cUa or garzGD13940 (together with UAS-Dicer2 to enhance the RNAi efficiency).

      The light-dependent retinal degeneration as well as the elevated number of rhodopsin1-containing vesicles per ommatidium characteristic for norpA36 homozygous adults can be partially suppressed by expression of PldScer\UAS.cRa under the control of Scer\GAL4ninaE.PU in the mutant background.

      (Thakur et al., 2016)

      Co-expression of CG11426Scer\UAS.T:Avic\GFP partially rescues the rhabdomere degeneration caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF.

      Co-expression of garzEP2028 strongly enhances the retina phenotype caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF; only one or two poorly developed rhabdomeres are seen.

      Co-expression of Gap69CScer\UAS.cRa partially the rhabdomere defects caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF.

      Co-expression of sizP2.Scer\UAS does not enhance the photoreceptor cell phenotypes caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF.

      Arf51F4 does not suppress the photoreceptor cell phenotypes caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF.

      Animals co-expressing both PldScer\UAS.cRa and α-AdaptindsRNA.Scer\UAS.cUa under the control of Scer\GAL4GMR.PF have virtually no detectable rhabdomeres, and the cell bodies of the photoreceptors continue to show accumulation of membrane transport intermediates.

      A shi1 background at 23[o]C does not suppress the rhabdomere defects caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF.

      There is a dramatic increase in rhabdomere size in flies expressing PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF in a sktlΔ1-1/sktlΔ20 background compared to flies expressing PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF in a wild-type background.

      (Raghu et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      PldUAS.cRa/Scer\GAL4hs.PU rescues Pld3.1

      (Panda et al., 2018)

      PldUAS.cRa/Scer\GAL4hs.PU rescues Pld3.1

      (Thakur et al., 2016)
      Comments

      The light-dependent retinal regeneration observed in Pld3.1 homozygous adults is rescued by expression of PldScer\UAS.cRa under the control of Scer\GAL4hs.PU in the mutant background.

      (Thakur et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      PldScer\UAS.cRa
      PldUAS.cRa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Raghu
      Secondary FlyBase IDs
        References (5)