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General Information
Symbol
Dmel\peloPB60
Species
D. melanogaster
Name
FlyBase ID
FBal0324962
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 107 bp deletion in the second exon of pelo resulting from the imprecise excision of P{SUPor-P}peloKG06646 that leads to early translation termination.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{SUPor-P}peloKG06646 results in deletion of 107bp in the 2nd exon of pelo, resulting in an early stop codon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The testes of peloPB60 homozygotes show a significant and progressive decrease in germline stem cells, as well as meiosis defects, with abnormally large spermatids containing 4N or 2N chromosomal content, and large nebenkern, as compared to controls; unlike controls, individualization complexes are absent and the spermatid nuclei are scattered.

peloPB60 heterozygous males also show defects in late spermatogenesis, namely significantly fewer individualization complexes and randomly scattered spermatid nuclei along the testicular tube, despite of no changes in germline stem cell number, as compared to controls.

The testes of peloPB60/pelo1 transheterozygotes have significantly fewer germline stem cells than in controls and lack both elongated spermatid nuclei bundles and individualization complexes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

peloPB60 has male germline stem cell | progressive phenotype, non-enhanceable by HBS11/HBS1[+]

Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

pelo[+]/peloPB60 is an enhancer of sperm individualization complex phenotype of HBS11/HBS1172

Additional Comments
Genetic Interactions
Statement
Reference

The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo1/peloPB60 transheterozygous females can be partially rescued by expression of RpS30Scer\UAS.P\T.cYa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background.

Xenogenetic Interactions
Statement
Reference

The spermatogenesis defects but not male sterility characteristic for pelo1/peloPB60 transheterozygous flies can be partially rescued by expression of Mmus\PeloScer\UAS.P\T.cYa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 while the Scer\GAL4αTub84B.PL-driven expression restores male fertility to near wild-type level.

Complementation and Rescue Data
Comments

Expressing peloUASp.cXa under the control of Scer\GAL4VP16.nos.UTR fully rescues the male sterility and spermatogenesis defects (male germline stem cell number, individualization complex formation and spermatid nuclei elongation defects) observed in peloPB60/pelo1 transheterozygotes. Expressing peloP210A.UASp under the control of Scer\GAL4VP16.nos.UTR, however, fails to rescue the male sterility and individualization complex formation defects, and only partially rescues the male germline stem cell number and spermatid nuclei elongation defects.

The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo1/peloPB60 transheterozygous females can be partially rescued by expression of peloScer\UAS.P\T.cXa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background. Expression under the Scer\GAL4nos.UTR.T:Hsim\VP16 driver rescues the spermatogenesis defects (no individualization complexes and waste bags) as well as the male sterility, which can also bes rescued by Scer\GAL4αTub84B.PL-driven expression.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)