The spermatogenesis defects but not male sterility characteristic for pelo1/peloPB60 transheterozygous flies can be partially rescued by expression of Mmus\PeloScer\UAS.P\T.cYa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 while the Scer\GAL4αTub84B.PL-driven expression restores male fertility to near wild-type level.
Expressing peloUASp.cXa under the control of Scer\GAL4VP16.nos.UTR fully rescues the male sterility and spermatogenesis defects (male germline stem cell number, individualization complex formation and spermatid nuclei elongation defects) observed in peloPB60/pelo1 transheterozygotes. Expressing peloP210A.UASp under the control of Scer\GAL4VP16.nos.UTR, however, fails to rescue the male sterility and individualization complex formation defects, and only partially rescues the male germline stem cell number and spermatid nuclei elongation defects.
The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo1/peloPB60 transheterozygous females can be partially rescued by expression of peloScer\UAS.P\T.cXa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background. Expression under the Scer\GAL4nos.UTR.T:Hsim\VP16 driver rescues the spermatogenesis defects (no individualization complexes and waste bags) as well as the male sterility, which can also bes rescued by Scer\GAL4αTub84B.PL-driven expression.