FlyBase Allele Report: Dmel\pelo[PB60]

General Information

Symbol

Dmel\pelo^PB60

Species

D. melanogaster

Name

FlyBase ID

FBal0324962

Feature type

allele

Associated gene

Dmel\pelo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Li et al., 2019)

amorphic allele - molecular evidence

(Yang et al., 2015)

Mutagen

P-element activity

(Yang et al., 2015)

Progenitor genotype

P{SUPor-P}pelo^KG06646

(Yang et al., 2015)

Cytology

Description

Imprecise excision of P{SUPor-P}pelo^KG06646 results in deletion of 107bp in the 2nd exon of pelo, resulting in an early stop codon.

(Yang et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:9,701,273..9,701,379 [+]

Comment:

A 107 bp deletion in the second exon of pelo resulting from the imprecise excision of P{SUPor-P}pelo^KG06646 that leads to early translation termination.

(Xi, 2017.7.11)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | spermatogenesis

(Li et al., 2019)

decreased cell number | progressive | recessive | spermatogenesis

(Li et al., 2019)

decreased cell number | spermatogenesis (with pelo¹)

(Li et al., 2019)

male sterile (with pelo¹)

(Li et al., 2019)

sterile (with pelo¹)

(Yang et al., 2015)

Phenotype Manifest In

elongation stage spermatid

(Li et al., 2019)

male germline cyst

(Li et al., 2019)

male germline stem cell (with pelo¹)

(Li et al., 2019)

male germline stem cell | progressive

(Li et al., 2019)

Nebenkern

(Li et al., 2019)

spermatid

(Li et al., 2019)

sperm individualization complex

(Li et al., 2019)

sperm individualization complex (with pelo¹)

(Li et al., 2019)

testis (with pelo¹)

(Yang et al., 2015)

Detailed Description

Statement

Reference

The testes of pelo^PB60 homozygotes show a significant and progressive decrease in germline stem cells, as well as meiosis defects, with abnormally large spermatids containing 4N or 2N chromosomal content, and large nebenkern, as compared to controls; unlike controls, individualization complexes are absent and the spermatid nuclei are scattered.

pelo^PB60 heterozygous males also show defects in late spermatogenesis, namely significantly fewer individualization complexes and randomly scattered spermatid nuclei along the testicular tube, despite of no changes in germline stem cell number, as compared to controls.

The testes of pelo^PB60/pelo¹ transheterozygotes have significantly fewer germline stem cells than in controls and lack both elongated spermatid nuclei bundles and individualization complexes.

(Li et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

pelo^PB60 has decreased cell number | spermatogenesis | recessive | progressive phenotype, non-enhanceable by HBS1¹/HBS1[+]

(Li et al., 2019)

Suppressed by

Statement

Reference

pelo¹/pelo^PB60 has female sterile phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/RpS30^UASp.cYa

(Yang et al., 2015)

pelo¹/pelo^PB60 has male sterile phenotype, suppressible by Mmus\Pelo^UASp.cYa/Scer\GAL4^αTub84B.PL

(Yang et al., 2015)

NOT suppressed by

Statement

Reference

pelo¹/pelo^PB60 has male sterile phenotype, non-suppressible by Mmus\Pelo^UASp.cYa/Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

pelo^PB60 has elongation stage spermatid phenotype, enhanceable by HBS1¹/HBS1¹⁷²

(Li et al., 2019)

pelo^PB60 has sperm individualization complex phenotype, enhanceable by HBS1¹/HBS1¹⁷²

(Li et al., 2019)

NOT Enhanced by

Statement

Reference

pelo^PB60 has male germline stem cell | progressive phenotype, non-enhanceable by HBS1¹/HBS1[+]

(Li et al., 2019)

Suppressed by

Statement

Reference

pelo¹/pelo^PB60 has testis phenotype, suppressible | partially by Mmus\Pelo^UASp.cYa/Scer\GAL4^αTub84B.PL

(Yang et al., 2015)

Enhancer of

Statement

Reference

pelo[+]/pelo^PB60 is an enhancer of individualization stage spermatid phenotype of HBS1¹/HBS1¹⁷²

(Li et al., 2019)

pelo[+]/pelo^PB60 is an enhancer of sperm individualization complex phenotype of HBS1¹/HBS1¹⁷²

(Li et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo¹/pelo^PB60 transheterozygous females can be partially rescued by expression of RpS30^{Scer\UAS.P\T.cYa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the mutant background.

(Yang et al., 2015)

Xenogenetic Interactions

Statement

Reference

The spermatogenesis defects but not male sterility characteristic for pelo¹/pelo^PB60 transheterozygous flies can be partially rescued by expression of Mmus\Pelo^{Scer\UAS.P\T.cYa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} while the Scer\GAL4^αTub84B.PL-driven expression restores male fertility to near wild-type level.

(Yang et al., 2015)

Complementation and Rescue Data

Rescued by

pelo¹/pelo^PB60 is rescued by pelo^UASp.cXa/Scer\GAL4^{VP16.nanos.UTR}

(Li et al., 2019)

pelo¹/pelo^PB60 is rescued by pelo^UASp.cXa/Scer\GAL4^αTub84B.PL

(Yang et al., 2015)

Partially rescued by

pelo¹/pelo^PB60 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/pelo^P210A.UASp

(Li et al., 2019)

pelo¹/pelo^PB60 is partially rescued by pelo^UASp.cXa/Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2015)

Comments

Expressing pelo^UASp.cXa under the control of Scer\GAL4^VP16.nos.UTR fully rescues the male sterility and spermatogenesis defects (male germline stem cell number, individualization complex formation and spermatid nuclei elongation defects) observed in pelo^PB60/pelo¹ transheterozygotes. Expressing pelo^P210A.UASp under the control of Scer\GAL4^VP16.nos.UTR, however, fails to rescue the male sterility and individualization complex formation defects, and only partially rescues the male germline stem cell number and spermatid nuclei elongation defects.

(Li et al., 2019)

The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo¹/pelo^PB60 transheterozygous females can be partially rescued by expression of pelo^{Scer\UAS.P\T.cXa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the mutant background. Expression under the Scer\GAL4^{nos.UTR.T:Hsim\VP16} driver rescues the spermatogenesis defects (no individualization complexes and waste bags) as well as the male sterility, which can also bes rescued by Scer\GAL4^αTub84B.PL-driven expression.

(Yang et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

68149

w^*; pelo^PB60/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pelo^PB60

(Li et al., 2019, Xi, 2017.7.11, Yang et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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