FlyBase Allele Report: Dmel\pelo[1]

P{PZ} is inserted into an intron in the pelo transcription unit. The insertion site lies 572 nucleotides 5' of an exon which begins at amino acid 319.

(Eberhart and Wasserman, 1995)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:9,706,076..9,706,076 [+]

(Xi et al., 2005, Spradling et al., 1999, Eberhart and Wasserman, 1995, BDGP Project Members, 1994-1999, Castrillon et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Castrillon et al., 1993)

abnormal meiotic cell cycle | recessive

(Eberhart and Wasserman, 1995)

abnormal size | oogenesis

(Xi et al., 2005)

decreased cell number | oogenesis

(Xi et al., 2005)

decreased cell number | oogenesis (with Df(2L)s1402)

(Xi et al., 2005)

decreased cell number | spermatogenesis (with pelo^PB60)

(Li et al., 2019)

decreased occurrence of cell division | oogenesis

(Xi et al., 2005)

female semi-sterile

(Eberhart and Wasserman, 1995)

female sterile

(Xi et al., 2005)

female sterile | cold sensitive

(Castrillon et al., 1993)

increased cell death

(Xi et al., 2005)

increased cell death | oogenesis

(Xi et al., 2005)

male sterile

male sterile (with Df(2L)30A-C)

(Kahsai and Cook, 2018)

male sterile (with Df(2L)N22-3)

(Kahsai and Cook, 2018)

male sterile (with Df(2L)N22-5)

(Kahsai and Cook, 2018)

male sterile (with Df(2L)N22-14)

(Kahsai and Cook, 2018)

male sterile (with Df(2L)s1402)

(Kahsai and Cook, 2018)

male sterile (with pelo^PB60)

(Li et al., 2019)

male sterile | recessive

(Spradling et al., 1999)

partially lethal - majority die

sterile (with pelo^PB60)

(Yang et al., 2015)

viable

(Eberhart and Wasserman, 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

The testes of pelo^PB60/pelo¹ transheterozygotes have significantly fewer germline stem cells than in controls and lack both elongated spermatid nuclei bundles and individualization complexes.

(Li et al., 2019)

pelo¹/pelo^PB60 transheterozygous mutants are practically sterile - the females lay only a few eggs that show a very low hatching rate, the males show severe spermatogenesis defects (no individualization complexes or waste bags are detectable in the testes).

(Yang et al., 2015)

pelo¹ mutant adults display resistance (higher survival rate) to Drosophila C Virus infection compared to controls.

(Wu et al., 2014)

The germaria of pelo¹ females have a lower number of germline stem cells (GSCs) that heterozygous controls; 7-day-old pelo¹ germaria have an average of 0.47 GSCs (vs. 2.4 GSCs in controls) with over half of them containing no GSC. pelo¹ germaria that do have 2 GSCs are small and contain very few germline cysts. No dying GSCs can be detected in pelo¹ germaria but dying cysts are observed. A lower amount of pelo¹ GSCs incorporate BrdU compared to controls, showing that GSC division is reduced. The self-renewal of GSCs is also affected in pelo¹ mutants as 55% of GSCs are maintained over a 3 week period in wild-type controls while over 97% of GSCs are lost over the same period in the mutants. pelo¹/Df(2L)s1402 females also exhibit a low number of GSCs. The formation and survival of somatic cells in the germarium is not affected in pelo¹ mutants. Mutant follicle cell clones exhibit a very minor phenotype, appearing slightly thinner than wild-type follicle cells. pelo¹ females are partially fertile after eclosion but quickly lose their fertility. This is reflected by the presence of some mature eggs in 2-day-old mutant ovaries, while 7-day-old ovaries rarely contain egg chambers that have progressed beyond stage 9. The early stage egg chambers, which contain an oocyte, exhibit condensed nurse cell DNA, suggesting that they are undergoing apoptosis, and are often smaller than wild-type egg chambers. pelo¹ germline cysts also fail to grow to a normal size and become apoptotic.

(Xi et al., 2005)

Homozygotes are fully viable at 18^oC and 25^oC, but have reduced viability in crowded conditions. Homozygous males show cell cycle arrest in spermatocytes just before, or very early in, the first meiotic cell division. Other aspects of spermatogenesis continue normally, resulting in 4N spermatids. Phenotype closely resembles that of twe¹. Spindle formation and nuclear envelope breakdown fail, but chromosome condensation proceeds normally. In some homozygous adults, eyes are roughened, with disordered ommatidial arrays and bristles. Eyes tend to be smaller than in wild-type siblings. Homozygous females have reduced fertility, and ovaries are reduced in size. The mitotic zone of the germarium appears disorganized and often contains degenerating cells.

(Eberhart and Wasserman, 1995)

Meiosis does not occur, abnormally large nebenkern form in later spermatocytes. Semi-lethal.

(Castrillon et al., 1993)

cold-sensitive. female-sterile. Meiosis does not occur; abnormally large Nebenkern forms in late spermatocytes.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pelo¹/pelo^PB60 has female sterile phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/RpS30^UASp.cYa

(Yang et al., 2015)

pelo¹/pelo^PB60 has male sterile phenotype, suppressible by Mmus\Pelo^UASp.cYa/Scer\GAL4^αTub84B.PL

(Yang et al., 2015)

NOT suppressed by

Statement

Reference

pelo¹/pelo^PB60 has male sterile phenotype, non-suppressible by Mmus\Pelo^UASp.cYa/Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2015)

pelo¹ has decreased cell number | oogenesis phenotype, non-suppressible by bam^Δ86

(Xi et al., 2005)

Suppressor of

Statement

Reference

pelo[+]/pelo¹ is a suppressor | partially of neoplasia phenotype of Scer\GAL4^C587, dpp^UAS.cNa

(Xi et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

pelo¹/pelo^PB60 has testis phenotype, suppressible | partially by Mmus\Pelo^UASp.cYa/Scer\GAL4^αTub84B.PL

(Yang et al., 2015)

Suppressor of

Statement

Reference

pelo[+]/pelo¹ is a suppressor | partially of female germline stem cell phenotype of Scer\GAL4^C587, dpp^UAS.cNa

(Xi et al., 2005)

pelo¹ is a suppressor of female germline stem cell phenotype of bam^Δ86

(Xi et al., 2005)

NOT Suppressor of

Statement

Reference

pelo[+]/pelo¹ is a non-suppressor of female germline stem cell phenotype of bam^Δ86

(Xi et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo¹/pelo^PB60 transheterozygous females can be partially rescued by expression of RpS30^{Scer\UAS.P\T.cYa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the mutant background.

(Yang et al., 2015)

In pelo¹;bam^Δ86 double mutant germaria, germ cells are able to differentiate as cystocytes can form. This is evidenced by branched fusomes, although the morphology of these fusomes is abnormal. However, some germ cells remain undifferentiated, as abnormally enlarged spectrosomes persist. Germ cell differentiation is not rescued when bam^Δ86 homozygous mutants are pelo¹/+; these germaria also show the rapid loss of GSCs observed in pelo¹ germaria. When dpp^Scer\UAS.cNa is expressed under the control of Scer\GAL4^C587 in a pelo¹/+ background, 64% of ovarioles no longer show the stem cell tumour phenotype seen when dpp^Scer\UAS.cNa is expressed in a wild-type background. These ovarioles contain differentiated germline cysts, developing egg chambers and mature eggs. When dpp^Scer\UAS.cNa is expressed in a pelo¹ homozygous background, the tumour phenotype is further suppressed.

(Xi et al., 2005)

Xenogenetic Interactions

Statement

Reference

The spermatogenesis defects but not male sterility characteristic for pelo¹/pelo^PB60 transheterozygous flies can be partially rescued by expression of Mmus\Pelo^{Scer\UAS.P\T.cYa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} while the Scer\GAL4^αTub84B.PL-driven expression restores male fertility to near wild-type level.

(Yang et al., 2015)

Complementation and Rescue Data

Rescued by

pelo¹/pelo^PB60 is rescued by pelo^UASp.cXa/Scer\GAL4^{VP16.nanos.UTR}

(Li et al., 2019)

pelo¹/pelo^PB60 is rescued by pelo^UASp.cXa/Scer\GAL4^αTub84B.PL

(Yang et al., 2015)

pelo¹ is rescued by pelo^UASp.cXa/Scer\GAL4^{VP16.nanos.UTR}

(Xi et al., 2005)

pelo¹ is rescued by Scer\GAL4^{VP16.nanos.UTR}/pelo^{nls.UASp.Tag:FLAG,Tag:MYC}

(Xi et al., 2005)

pelo¹ is rescued by pelo^{UASp.Tag:FLAG,Tag:MYC}/Scer\GAL4^{VP16.nanos.UTR}

(Xi et al., 2005)

pelo¹ is rescued by pelo^+t15

(Eberhart and Wasserman, 1995)

Partially rescued by

pelo¹/pelo^PB60 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/pelo^P210A.UASp

(Li et al., 2019)

pelo¹/pelo^PB60 is partially rescued by pelo^UASp.cXa/Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2015)

pelo¹ is partially rescued by pelo^UASp.cXa

(Xi et al., 2005)

pelo¹ is partially rescued by pelo^UASp.cXa/Scer\GAL4^C587

(Xi et al., 2005)

Not rescued by

pelo¹ is not rescued by pelo^{nls.UASp.Tag:FLAG,Tag:MYC}

(Xi et al., 2005)

pelo¹ is not rescued by Scer\GAL4^C587/pelo^{nls.UASp.Tag:FLAG,Tag:MYC}

(Xi et al., 2005)

Comments

Expressing pelo^UASp.cXa under the control of Scer\GAL4^VP16.nos.UTR fully rescues the male sterility and spermatogenesis defects (male germline stem cell number, individualization complex formation and spermatid nuclei elongation defects) observed in pelo^PB60/pelo¹ transheterozygotes. Expressing pelo^P210A.UASp under the control of Scer\GAL4^VP16.nos.UTR, however, fails to rescue the male sterility and individualization complex formation defects, and only partially rescues the male germline stem cell number and spermatid nuclei elongation defects.

(Li et al., 2019)

The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo¹/pelo^PB60 transheterozygous females can be partially rescued by expression of pelo^{Scer\UAS.P\T.cXa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the mutant background. Expression under the Scer\GAL4^{nos.UTR.T:Hsim\VP16} driver rescues the spermatogenesis defects (no individualization complexes and waste bags) as well as the male sterility, which can also bes rescued by Scer\GAL4^αTub84B.PL-driven expression.

(Yang et al., 2015)

The GSC loss phenotype in pelo¹ females is partially recued by leaky expression of the pelo^{Scer\UAS.P\T.cXa} transgene without a driver; expression of Scer\GAL4^C587 in the somatic cells of the germarium confers very limited extra rescue compared to that conferred by the transgene alone.

(Xi et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

11757

P{PZ}pelo¹ cn¹/CyO; ry⁵⁰⁶

Kyoto

108877

P{PZ}pelo¹ cn¹/CyO; ry⁵⁰⁶

101998

P{PZ}pelo¹ cn¹ / CyO ; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: Pka-C1⁰¹²⁷². Complements: ms(2)30C⁰⁷⁸²².

(BDGP Project Members, 1994-1999)

P\T^Δ2-3-induced reversion correlates precise excision of the P{PZ} with reversion of the pelo¹ mutant phenotype. Phenotype of pelo¹/Df(2L)30A-C is indistinguishable from that of pelo¹ homozygotes.

(Eberhart and Wasserman, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ms(2)01559

(Spradling et al., 1999)

ms(2)01559⁰¹⁵⁵⁹

pelo⁰¹⁵⁵⁹

pelo¹

(Blatt et al., 2021, Li et al., 2019, Kahsai and Cook, 2018, Yang et al., 2015, Wu et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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