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General Information
Symbol
Dmel\HBS11
Species
D. melanogaster
Name
FlyBase ID
FBal0346868
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

2307bp deletion resulting from the imprecise excision of P{EPgy2}HBS1EY09557 that removes most of the coding region of HBS1.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

2307bp deletion (coordinates 3L:1863069..1865375 ) that removes the entire HBS1 coding region. Generated by imprecise excision of P{EPgy2}HBS1EY09557.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

HBS11 homozygous males show grossly normal testis morphology but lack sperm in the seminal vesicle and the seminal vesicles are much smaller than heterozygous controls; spermatogonia and spermatocytes show comparable sizes to controls, but spermatid cysts frequently show abnormal ratios of nebenkern to nucleus ( 1:1 , 1:2 , 1:3 to 1:4 , instead of the expected 1:1 ratio), abnormal large nebenkern and small or micronuclei, which indicates failure of cytokinesis and chromosome mis-segregation during spermatogenesis; spindle microtubules frequently fail to converge into the poles and are splayed outward; there are defects in later spermatogenesis, as individualization complexes are absent and the spermatid nuclei are randomly scattered along the testicular tube, as compared to controls; there are no change in the numbers of germline stem cells and spermatogonial cells (Bam-GFP positive cells), as compared to controls.

HBS11 heterozygotes show a similar number of individualization complexes to wild-type controls.

HBS11/HBS1172 transheterozygous males also have late spermatogenesis defects, namely significantly fewer individualization complexes and randomly scattered spermatid nuclei along the testicular tube, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference

HBS11/HBS1[+] is a non-enhancer of male germline stem cell | progressive phenotype of peloPB60

HBS11/HBS1[+] is a non-enhancer of male germline stem cell | progressive phenotype of peloPA13

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
HBS11
Name Synonyms
Secondary FlyBase IDs
    References (1)