FlyBase Allele Report: Dmel\ifc[KO]

General Information

Symbol

Dmel\ifc^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0344249

Feature type

allele

Associated gene

Dmel\ifc

Associated Insertion(s)

Carried in Construct

Also Known As

ifc-KO

Key Links

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Zhu et al., 2025)

amorphic allele - molecular evidence

(Jung et al., 2017)

Mutagen

CRISPR/Cas9

(Jung et al., 2017)

Progenitor genotype

Cytology

Description

Deletion of the entire ifc open reading frame.

(Jung et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Tzou et al., 2021, Jung et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is ameliorated by Nox^HMS00429

(Tzou et al., 2021)

is ameliorated by Nox^HMS00691

(Tzou et al., 2021)

is ameliorated by Rac1^JF02813

(Tzou et al., 2021)

is ameliorated by Sod1^UAS.cWa

(Tzou et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Jung et al., 2017)

abnormal neurophysiology | adult stage | progressive | somatic clone | conditional

(Jung et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Whole eye ifc^KO homozygous clones in an otherwise mosaic individual show a progressive loss of rhabdomeres accompanied with distorted photoreceptor margins when under constant light conditions. Likewise, electroretinogram depolarization readings progressively decrease when under constant light conditions. These defects are not observed at eclosion nor under constant darkness. Clones within mosaic eyes also show the same significant decrease in rhabdomere number, associated with apoptotic bodies and increased lipid droplet-containing autophagosomes (autophagosomes: Atg8a-positive puncta; lipid-droplets: Bodipy-positive puncta).

Whole-eye ifc^KO homozygous clones also show a significant increase in the number of lipid droplets (Bodipy-positive puncta), as compared to controls.

(Jung et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ifc^KO has increased mortality during development phenotype, suppressible | partially by schlank^GD15550/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO has abnormal neurophysiology | somatic clone | adult stage | progressive | conditional phenotype, suppressible by schlank^GD15550/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO has increased mortality during development phenotype, suppressible | partially by Hsap\DEGS1^UAS.cJa/Scer\GAL4^αTub84B.PL

(Jung et al., 2017)

ifc^KO has increased mortality during development phenotype, suppressible | partially by Hsap\DEGS1^UAS.cJa/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^repo/Hsap\DEGS1^UAS.cJa

(Jung et al., 2017)

ifc^KO has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^Mef2.PR/Hsap\DEGS1^UAS.cJa

(Jung et al., 2017)

NOT suppressed by

Statement

Reference

ifc^KO has increased mortality during development phenotype, non-suppressible by schlank^GD15550/Scer\GAL4^αTub84B.PL

(Jung et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

ifc^KO has eye | somatic clone phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO has retina | somatic clone | adult stage | progressive | conditional phenotype, suppressible by schlank^GD15550/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO has autophagosome | somatic clone | adult stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO has rhabdomere of eye photoreceptor cell | somatic clone | adult stage | progressive | conditional phenotype, suppressible by schlank^GD15550/Scer\GAL4^elav.PLu

(Jung et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

ifc^KO is partially rescued by Scer\GAL4^αTub84B.PL/ifc^UAS.cJa

(Jung et al., 2017)

ifc^KO is partially rescued by Scer\GAL4^elav.PLu/ifc^UAS.cJa

(Jung et al., 2017)

ifc^KO is partially rescued by Scer\GAL4^repo/ifc^UAS.cJa

(Jung et al., 2017)

ifc^KO is partially rescued by Scer\GAL4^Mef2.PR/ifc^UAS.cJa

(Jung et al., 2017)

ifc^KO is partially rescued by ifc^UAS.mCherry/Scer\GAL4^αTub84B.PL

(Jung et al., 2017)

ifc^KO is partially rescued by ifc^UAS.mCherry/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO is partially rescued by Scer\GAL4^repo/ifc^UAS.mCherry

(Jung et al., 2017)

ifc^KO is partially rescued by Scer\GAL4^Mef2.PR/ifc^UAS.mCherry

(Jung et al., 2017)

Not rescued by

ifc^KO is not rescued by ifc^UAS.CFP/Scer\GAL4^αTub84B.PL

(Jung et al., 2017)

ifc^KO is not rescued by ifc^UAS.CFP/Scer\GAL4^elav.PLu

(Jung et al., 2017)

ifc^KO is not rescued by ifc^UAS.CFP/Scer\GAL4^repo

(Jung et al., 2017)

ifc^KO is not rescued by ifc^UAS.CFP/Scer\GAL4^Mef2.PR

(Jung et al., 2017)

Comments

Expressing ifc^UAS.cJa under the control of Scer\GAL4^αTub84B.PL partially rescues the second instar larval lethality of ifc^KO homozygotes, so that most individuals reach the pupal stage or adulthood; expressing under the control of either Scer\GAL4^elav.PLu, Scer\GAL4^repo or Scer\GAL4^Mef2.PR only allows some individuals to reach pupal stage or adulthood.

Expressing ifc^UAS.mCherry under the control of Scer\GAL4^αTub84B.PL partially rescues the second instar larval lethality of ifc^KO homozygotes, so that most individuals reach the pupal stage or adulthood; expressing under the control of either Scer\GAL4^elav.PLu, Scer\GAL4^repo or Scer\GAL4^Mef2.PR only allows some individuals to reach pupal stage or adulthood.

(Jung et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from second-site mutation(s) on the chromosome, affecting the MED15, lwr, Nle and Sf3b1 complementation groups. Since these four genes reside within approximately 90kb near the telomere in 2L, the second site lesion may be a small deletion that removes all four genes.

(Zhu et al., 2025)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

des1^KO

(Ziegler et al., 2025)

ifc-KO

(Tzou et al., 2025, Zhu et al., 2025, Tzou et al., 2021, Wu et al., 2021, Jung et al., 2017)

ifc^KO

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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