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General Information
Symbol
Hsap\SOD1UAS.cWa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Watson
FlyBase ID
FBal0248884
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hSOD1, UAS-SOD1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Carries a corrected K75R amino acid substitution to match the canonical Hsap\SOD1 open reading frame.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Animals expressing Hsap\SOD1Scer\UAS.cWa in motor neurons under the control of Scer\GAL4D42 perform similarly to wild-type in larval turning assays.

park25 mutant flies expressing Hsap\SOD1Scer\UAS.cWa under the control of Scer\GAL4twi.PG do not show a difference in survival compared to park25 flies, up to 4 days of age. However, at 11 days of age only 10% of the park25 flies remain, compared to 80% of the park25 flies expressing Hsap\SOD1Scer\UAS.cWa under the control of Scer\GAL4twi.PG. None of the park25 flies survive to 17 days of age, at which time point approximately 80% of Hsap\SOD1Scer\UAS.cWa-expressing flies are still alive.

Flies overexpressing Hsap\SOD1Scer\UAS.cWa driven by Scer\GAL4D42 in motor neurons show a progressive loss of climbing ability starting at 21 days after eclosion.

Flies overexpressing Hsap\SOD1Scer\UAS.cWa driven by Scer\GAL4D42 show no detectable loss of neuronal nuclei over time.

No anatomical degeneration of the retina occur in flies overexpressing Hsap\SOD1Scer\UAS.cWa driven by Scer\GAL4GMR.PF.

Synaptic transmission along the giant fibre motor pathway is abnormal in flies overexpressing Hsap\SOD1Scer\UAS.cWa driven by Scer\GAL4D42 in motor neurons.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
Statement
Reference

Hsap\SOD1UAS.cWa/Scer\GAL4twi.PG is a suppressor of short lived | chemical conditional phenotype of park25

Hsap\SOD1UAS.cWa/Scer\GAL4twi.PG is a suppressor of long lived | recessive | chemical conditional phenotype of park25

NOT Suppressor of
Other
Phenotype Manifest In
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of Hsap\SOD1Scer\UAS.cWa enhances the toxicity of Scer\GAL4GMR.PU>Hsap\MAPTR406W.Scer\UAS in Drosophila compound eyes. The rough eye phenotype caused by Hsap\MAPTR406W.Scer\UAS-expression becomes more severe, and the ommatidia are more severely fused and irregular.

Co-expression of Hsap\SOD1Scer\UAS.cWa shortens the lifespan of files expressing Scer\GAL4elav.PU>Hsap\MAPTR406W.Scer\UAS and exacerbates their movement impairment.

Co-expression of Hsap\SOD1Scer\UAS.cWa exacerbates the brain damage caused by the expression of Scer\GAL4elav.PU>Hsap\MAPTR406W.Scer\UAS. The number of vacuoles is significantly increased in the double-transgenic flies compared with Hsap\MAPTR406W.Scer\UAS-expression alone.

park25 mutant flies expressing Hsap\SOD1Scer\UAS.cWa under the control of Scer\GAL4twi.PG do not show a difference in survival compared to park25 flies, up to 4 days of age. However, at 11 days of age only 10% of the park25 flies remain, compared to 80% of the park25 flies expressing Hsap\SOD1Scer\UAS.cWa under the control of Scer\GAL4twi.PG. None of the park25 flies sruvvie to 17 days of age, at which time point approximately 80% of Hsap\SOD1Scer\UAS.cWa-expressing flies are still alive.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Hsap\SOD1Scer\UAS.cWa
Hsap\SOD1UAS.cWa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Watson
Secondary FlyBase IDs
    References (12)