FB2026_02 , released June 18, 2026
Allele: Scer\GAL4Acox1-CR00998-TG4.1
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General Information
Symbol
Scer\GAL4Acox1-CR00998-TG4.1
Species
S. cerevisiae
Name
FlyBase ID
FBal0352147
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{CRIMIC.TG4.1}Acox1CR00998-TG4.1
Carried in Construct
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{CRIMIC.TG4.1}Acox1CR00998-TG4.1
Cytology
Description

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into CG5009, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: ATGTACATGTAGGTCTGTCGGGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 5bp of genomic sequence flanking the insertion site.

(Gene Disruption Project members, 2018-)
Allele components
Component
Use(s)
Inserted element
TI{CRIMIC.TG4.1}
Encoded product / tool
GAL4
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage -- adult stage
      CNS glial cell
      (Chung et al., 2020)
      adult stage
      adult glial cell
      (Chung et al., 2020)
      Additional Information
      Statement
      Reference

      ScerGAL4ACOX1-CR00998-TG4.1 drives expression in glial cells of the larval CNS, and in most adult glial cells; expression is colocalized repo.

      (Chung et al., 2020)
       
      Marker for
      Reflects expression of
      Acox1
      (Chung et al., 2020)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Scer\GAL4ACOX1-CR00998-TG4.1
      Scer\GAL4Acox1-CR00998-TG4.1
      Scer\GAL4CG5009-CR00998-TG4.1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)