FlyBase Allele Report: Dmel\wg[CTD.UAS.Tag:MYC]

General Information

Symbol

Dmel\wg^{CTD.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0353154

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.CTD.myc}

Key Links

Transgenic product class

inframe_deletion

(Won et al., 2019)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Won et al., 2019)

Mutagen

in vitro construct

(Won et al., 2019)

Progenitor genotype

Carried in construct

P{UAS-wg.CTD.myc}

Cytology

Description

UASt regulatory sequences drive expression of wg amino acids 1-22 and 245-468. A Tag:MYC epitope has been inserted in between residues 367 and 368.

(Won et al., 2019)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^ci.PC

(Won et al., 2019)

abnormal size | adult stage, with Scer\GAL4^en.PU

(Won et al., 2019)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Won et al., 2019)

abnormal size | larval stage, with Scer\GAL4^en.PU

(Won et al., 2019)

increased occurrence of cell division | larval stage, with Scer\GAL4^ptc.PU

(Won et al., 2019)

some die during larval stage, with Scer\GAL4^ptc.PU

(Won et al., 2019)

some die during pupal stage, with Scer\GAL4^nub.PU

(Won et al., 2019)

some die during pupal stage, with Scer\GAL4^ptc.PU

(Won et al., 2019)

visible | adult stage, with Scer\GAL4^ci.PC

(Won et al., 2019)

visible | adult stage, with Scer\GAL4^en.PU

(Won et al., 2019)

visible | adult stage, with Scer\GAL4^nub.PU

(Won et al., 2019)

visible | adult stage, with Scer\GAL4^ptc.PU

(Won et al., 2019)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing disc, with Scer\GAL4^ptc.PU

(Won et al., 2019)

eye, with Scer\GAL4^ci.PC

(Won et al., 2019)

wing | adult stage, with Scer\GAL4^en.PU

(Won et al., 2019)

wing | adult stage, with Scer\GAL4^nub.PU

(Won et al., 2019)

wing | adult stage, with Scer\GAL4^ptc.PU

(Won et al., 2019)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Won et al., 2019)

Detailed Description

Statement

Reference

The continual expression of wg^{CTD.UAS.Tag:MYC} under the control of Scer\GAL4^en.PU produces small and deformed wings, and the posterior region of the wing disc is smaller than the anterior region. However, acute expression for 10h during mid-third instar larval stage (under the additional control of Gal80[ts]) produces significantly larger wings than in controls.

The continual expression of wg^{CTD.UAS.Tag:MYC} under the control of Scer\GAL4^nub.PU also produces small and deformed wings, without ectopic bristles in the wing margin. Transient expression of wg^{CTD.UAS.Tag:MYC} under the combined control of Scer\GAL4^nub.PU and Gal80[ts] does not induce ectopic bristles in the wing margin.

The continual expression of wg^{CTD.UAS.Tag:MYC} under the control of Scer\GAL4^ptc.PU leads to significantly increased mitotic index in the wing disc ptc-positive domain, and produces notched wings.

The continual expression under the control of either Scer\GAL4^wg-Gal4 or Scer\GAL4^bbg-C96 leads to normal wings, without notches.

The continual expression of wg^{CTD.UAS.Tag:MYC} under the control of Scer\GAL4^ci.PC produces small and rough eyes.

Transient expression of wg^{CTD.UAS.Tag:MYC} under the combined control of Scer\GAL4^nub.PU and Gal80[ts] does not induce ectopic bristles in the wing margin.

(Won et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, wg^{CTD.UAS.Tag:MYC} has visible | adult stage phenotype, suppressible by sona^RNAi.UAS.1/Scer\GAL4^ptc.PU

(Won et al., 2019)

Scer\GAL4^nub.PU, wg^{CTD.UAS.Tag:MYC} has abnormal size | adult stage phenotype, suppressible | partially by sgg^UAS.cBa/Scer\GAL4^nub.PU