FlyBase Allele Report: Scer\GAL4[Cdkl-CR70087-TG4.2]

General Information

Symbol

Scer\GAL4^{Cdkl-CR70087-TG4.2}

Species

S. cerevisiae

Name

FlyBase ID

FBal0390606

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{CRIMIC.TG4.2}Cdkl^{CR70087-TG4.2}

Carried in Construct

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.TG4.2}Cdkl^{CR70087-TG4.2}

Cytology

Description

A TI{CRIMIC.TG4.2} DNA cassette has been inserted into CG7236, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.2} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: CGGAATTCCCACCCCGATTGCGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.2}

gene trap

RMCE target element

Encoded product / tool

GAL4

binary expression system - driver

Also carries

attP

integrase target site

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion_site

2L:5,712,189..5,712,190 [+]

(Bereshneh et al., 2025, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | adult stage, with Hsap\CDKL1^{R169C.UAS.Tag:HA}

(Bereshneh et al., 2025)

abnormal heat stress response | adult stage, with Hsap\CDKL1^{V115A.UAS.Tag:HA}

(Bereshneh et al., 2025)

abnormal heat stress response | adult stage, with Hsap\CDKL2^E333VE.UAS

(Bereshneh et al., 2025)

abnormal heat stress response | adult stage, with Hsap\CDKL2^M215L.UAS

(Bereshneh et al., 2025)

abnormal heat stress response | adult stage, with Hsap\CDKL2^N468S.UAS

(Bereshneh et al., 2025)

abnormal heat stress response | adult stage, with Hsap\CDKL2^S119G.UAS

(Bereshneh et al., 2025)

bang sensitive, with Hsap\CDKL1^{R169C.UAS.Tag:HA}

(Bereshneh et al., 2025)

bang sensitive, with Hsap\CDKL1^{V115A.UAS.Tag:HA}

(Bereshneh et al., 2025)

bang sensitive, with Hsap\CDKL2^E333VE.UAS

(Bereshneh et al., 2025)

bang sensitive, with Hsap\CDKL2^M215L.UAS

(Bereshneh et al., 2025)

bang sensitive, with Hsap\CDKL2^N468S.UAS

(Bereshneh et al., 2025)

bang sensitive, with Hsap\CDKL2^S119G.UAS

(Bereshneh et al., 2025)

decreased rate of adult locomotory behavior, with Hsap\CDKL1^{R169C.UAS.Tag:HA}

(Bereshneh et al., 2025)

decreased rate of adult locomotory behavior, with Hsap\CDKL1^{V115A.UAS.Tag:HA}

(Bereshneh et al., 2025)

decreased rate of adult locomotory behavior, with Hsap\CDKL2^E333VE.UAS

(Bereshneh et al., 2025)

decreased rate of adult locomotory behavior, with Hsap\CDKL2^M215L.UAS

(Bereshneh et al., 2025)

decreased rate of adult locomotory behavior, with Hsap\CDKL2^N468S.UAS

(Bereshneh et al., 2025)

decreased rate of adult locomotory behavior, with Hsap\CDKL2^S119G.UAS

(Bereshneh et al., 2025)

short lived, with Hsap\CDKL1^{R169C.UAS.Tag:HA}

(Bereshneh et al., 2025)

short lived, with Hsap\CDKL1^{V115A.UAS.Tag:HA}

(Bereshneh et al., 2025)

short lived, with Hsap\CDKL2^E333VE.UAS

(Bereshneh et al., 2025)

short lived, with Hsap\CDKL2^M215L.UAS

(Bereshneh et al., 2025)

short lived, with Hsap\CDKL2^N468S.UAS

(Bereshneh et al., 2025)

short lived, with Hsap\CDKL2^S119G.UAS

(Bereshneh et al., 2025)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL1^UAS.Tag:HA is a suppressor of increased mortality during development phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^UAS.cBa/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of increased mortality during development phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{V115A.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of increased mortality during development phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{R169C.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of increased mortality during development phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^N468S.UAS is a suppressor of increased mortality during development phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL1^UAS.Tag:HA is a suppressor of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^UAS.cBa/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{R169C.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^E333VE.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^N468S.UAS is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{V115A.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^S119G.UAS is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^M215L.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{R169C.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^S119G.UAS is a suppressor | partially of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^E333VE.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^N468S.UAS is a suppressor | partially of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^M215L.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{V115A.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL1^UAS.Tag:HA is a suppressor | partially of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^UAS.cBa/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL1^UAS.Tag:HA is a suppressor of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^UAS.cBa/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{V115A.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{R169C.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL1^UAS.Tag:HA is a suppressor of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^UAS.cBa/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL5^UAS.cBa is a suppressor of decreased rate of adult locomotory behavior phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL5^UAS.cBa is a suppressor of abnormal heat stress response | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

NOT Suppressor of

Statement

Reference

Hsap\CDKL2^E333VE.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of partially lethal - majority die phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^S119G.UAS is a non-suppressor of partially lethal - majority die phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^M215L.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^E333VE.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^N468S.UAS is a non-suppressor of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^M215L.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of partially lethal - majority die phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{R169C.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{V115A.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^N468S.UAS is a non-suppressor of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^E333VE.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^M215L.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^S119G.UAS is a non-suppressor of short lived phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^S119G.UAS is a non-suppressor of abnormal neurophysiology | adult stage phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL1^UAS.Tag:HA is a suppressor of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^UAS.cBa/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{V115A.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL1^{R169C.UAS.Tag:HA}/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a suppressor | partially of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

NOT Suppressor of

Statement

Reference

Hsap\CDKL2^M215L.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^N468S.UAS is a non-suppressor of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Scer\GAL4^{Cdkl-CR70087-TG4.2}/Hsap\CDKL2^S119G.UAS is a non-suppressor of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Hsap\CDKL2^E333VE.UAS/Scer\GAL4^{Cdkl-CR70087-TG4.2} is a non-suppressor of antenna phenotype of Df(2L)ED270/Cdkl^{CR70087-TG4.2}

(Bereshneh et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98479

y¹ w^*; TI{CRIMIC.TG4.2}Cdkl^{CR70087-TG4.2}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cdkl^T2A-GAL4

(Bereshneh et al., 2025)

Scer\GAL4^{CG7236-CR70087-TG4.2}

Scer\GAL4^{Cdkl-CR70087-TG4.2}

Name Synonyms

Secondary FlyBase IDs

References (2)

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