FlyBase Allele Report: Dmel\N[RNAi.UAS.cUa]

General Information

Symbol

Dmel\N^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407456

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.RNAi.U}

Also Known As

UAS-Notch-RNAi, UAS-Notch^RNAi, N-IR, notch^RNAi, UAS-N-RNAi, UAS-NIRM, UAS-N^RNAi, UAS-Notch RNAi, N RNAi

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-N.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a N RNAi construct.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Ayyaz et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is ameliorated by Smox^JF02320

(Ayyaz et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP5130

(Yin and Xi, 2018)

dMP2 neuron | embryonic stage, with N^l1N-ts1, Scer\GAL4^repo-M1B

(Kuzina et al., 2011)

enteroblast | somatic clone, with Scer\GAL4^NP5130

(Xu et al., 2018)

enteroendocrine cell | adult stage, with Scer\GAL4^NP5130

(Yin and Xi, 2018)

enteroendocrine cell | somatic clone, with Scer\GAL4^NP5130

(Xu et al., 2018)

eo neuron | third instar larval stage, with Scer\GAL4^ap-md544

(Matsumoto et al., 2016)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Yin and Xi, 2018)

intestinal stem cell | somatic clone, with Scer\GAL4^NP5130

(Xu et al., 2018)

Malpighian tubule | somatic clone, with Scer\GAL4^NP5130

(Xu et al., 2018)

midgut | somatic clone, with Scer\GAL4^NP5130

(Xu et al., 2018)

tracheolar cell | wandering third instar larval stage, with Scer\GAL4^btl.PS

(Pitsouli and Perrimon, 2013)

vMP2 tract | embryonic stage, with N^l1N-ts1, Scer\GAL4^repo-M1B

(Kuzina et al., 2011)

wing disc | third instar larval stage, with Scer\GAL4^ap-md544

(Matsumoto et al., 2016)

wing margin, with Scer\GAL4^bbg-C96

(Singh et al., 2025)

Detailed Description

Statement

Reference

The clonal expression of N^VDRC.cUa under the control of Scer\GAL4^esg-NP5130 leads to a strong reduction in the number of progenitor cells and an increase in the number of enteroendocrine cells in the midgut region, as compared to controls; this expression in the renal tubule region also leads to a decrease in the number of progenitor cells, as compared to controls.

(Xu et al., 2018)

Adulthood-only expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP5130 (using Gal80[ts] for the temporal control of expression) leads to abnormal intestinal stem cell-like and enteroendocrine cell-like tumors in the adult midgut.

(Yin and Xi, 2018)

The expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^ap-md544 leads to third instar larval wing discs exhibiting increased number of supraalar neurons, as compared to controls.

(Matsumoto et al., 2016)

Expression of N^{dsRNA.UAS.cUa} under the control of Scer\GAL4^btl.PS (in combination with a Gal80[ts] transgene to overcome embryonic and larval lethality) in spiracular branch (SB) growth zones 1 and 2, leads to a reduction of number of SB tracheoblasts.

(Pitsouli and Perrimon, 2013)

Expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^repo-M1B enhances the N^l1N-ts1 early axon phenotype.

(Kuzina et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has decreased cell number | somatic clone phenotype, non-enhanceable by ct^HMS00924, Scer\GAL4^NP5130

(Xu et al., 2018)

Suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^bbg-C96 has visible | adult stage phenotype, suppressible by Awh^RNAi.UAS.cUa, Scer\GAL4^bbg-C96

(Singh et al., 2025)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has neoplasia | adult stage phenotype, suppressible by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

NOT suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has increased cell number | somatic clone phenotype, non-suppressible by ct^HMS00924, Scer\GAL4^NP5130

(Xu et al., 2018)

Enhancer of

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 is an enhancer of increased cell number | somatic clone phenotype of Scer\GAL4^NP5130, ct^HMS00924

(Xu et al., 2018)

Suppressor of

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^nub.PU is a suppressor | partially of neoplasia | larval stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^nub.PU, p53^UAS.DΔNp53

(Simón et al., 2014)

N^RNAi.UAS.cUa, Scer\GAL4^sd.PU is a suppressor | partially of neoplasia | larval stage phenotype of Scer\GAL4^sd.PU, p53^UAS.DΔNp53

(Simón et al., 2014)

N^RNAi.UAS.cUa, Scer\GAL4^sd.PU is a suppressor | partially of abnormal size | larval stage phenotype of Scer\GAL4^sd.PU, p53^UAS.DΔNp53

(Simón et al., 2014)

Scer\GAL4^insc-Mz1407/N^RNAi.UAS.cUa is a suppressor of abnormal neuroanatomy | recessive phenotype of AP-2α^SH0460

(Song and Lu, 2012)

Scer\GAL4^insc-Mz1407/N^RNAi.UAS.cUa is a suppressor of hyperplasia | recessive phenotype of AP-2α^SH0460

(Song and Lu, 2012)

Other

Statement

Reference

Dronc^{KO.-FRT.SunTag(Unk),Tag:HA,mCherry}/Dronc^KO, N^RNAi.UAS.cUa, Scer\GAL4^esg.PU has increased cell number | somatic clone | adult stage phenotype

(Arthurton et al., 2020)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has midgut | somatic clone phenotype, non-enhanceable by ct^HMS00924, Scer\GAL4^NP5130

(Xu et al., 2018)

Suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^bbg-C96 has wing margin phenotype, suppressible by Awh^RNAi.UAS.cUa, Scer\GAL4^bbg-C96

(Singh et al., 2025)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has adult midgut epithelium phenotype, suppressible by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has enteroendocrine cell | adult stage phenotype, suppressible by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

NOT suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has midgut | somatic clone phenotype, non-suppressible by ct^HMS00924, Scer\GAL4^NP5130

(Xu et al., 2018)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has enteroendocrine cell | somatic clone phenotype, non-suppressible by ct^HMS00924, Scer\GAL4^NP5130

(Xu et al., 2018)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has intestinal stem cell | adult stage phenotype, non-suppressible by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

Enhancer of

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 is an enhancer of adult ureter | somatic clone phenotype of Scer\GAL4^NP5130, ct^HMS00924

(Xu et al., 2018)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 is an enhancer of adult enteroendocrine cell | ectopic | somatic clone phenotype of Scer\GAL4^NP5130, ct^HMS00924

(Xu et al., 2018)

Suppressor of

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^sd.PU is a suppressor | partially of wing pouch phenotype of Scer\GAL4^sd.PU, p53^UAS.DΔNp53

(Simón et al., 2014)

N^RNAi.UAS.cUa, Scer\GAL4^sd.PU is a suppressor | partially of wing disc phenotype of Scer\GAL4^sd.PU, p53^UAS.DΔNp53

(Simón et al., 2014)

N^RNAi.UAS.cUa, Scer\GAL4^nub.PU is a suppressor | partially of wing disc phenotype of BacA\p35^UAS.cUa, Scer\GAL4^nub.PU, p53^UAS.DΔNp53

(Simón et al., 2014)

Scer\GAL4^insc-Mz1407/N^RNAi.UAS.cUa is a suppressor of larval neuroblast | increased number phenotype of AP-2α^SH0460

(Song and Lu, 2012)

Scer\GAL4^insc-Mz1407/N^RNAi.UAS.cUa is a suppressor of larval brain phenotype of AP-2α^SH0460

(Song and Lu, 2012)

NOT Suppressor of

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1

(Wang et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Adult posterior midgut clones specifically generated in esg-positive progenitors (ISCs and EBs), and which both express N^{dsRNA.UAS.cUa} under the control of Scer\GAL4^esg.PU and are Dronc^{KO.-FRT.SunTag(Unk),Tag:HA,mCherry}/Dronc^KO transheterozygous show increased proportion of progenitors.

(Arthurton et al., 2020)

The abnormal adult midgut enteroendocrine cell-like tumors, but not the intestinal stem cell-like tumors, resulting from the expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP5130 (using Gal80[ts] for the temporal control of expression) are suppressed by the co-expression of phyl^GD12579.

(Yin and Xi, 2018)

Additional expression of N^TRiP.cUa in individuals expressing p53^UAS.DΔNp53 under the control of Scer\GAL4^sd.PU reduces wing pouch protruding overgrowths and suppresses the size decrease of the wing disc.

Wing discs co-expressing Diap1^UAS.cUa and p53^UAS.DΔNp53 under the control of Scer\GAL4^sd.PU become smaller upon additional expression of N^TRiP.cUa.

(Simón et al., 2014)

Expression of N^VDRC.cUa under the control of Scer\GAL4^insc-Mz1407 completely suppresses the supernumerary neuroblast phenotype in α-Adaptin^SH0460 mutant larval brains.

(Song and Lu, 2012)

Xenogenetic Interactions

Statement

Reference

Additional expression of N^TRiP.cUa in individuals expressing BacA\p35^UAS.cUa and p53^UAS.DΔNp53 under the control of Scer\GAL4^nub.PU reduces tissue overgrowths in the wing disc.

(Simón et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

N^RNAi.UAS.cUa

N^RNAi

(Petkau et al., 2017)

N^TRiP.cUa

N^VDRC.cUa

N^{dsRNA.Scer\UAS.cUa}

N^{dsRNA.UAS.cUa}

notch^RNAi

(Cai et al., 2019)

Name Synonyms

Secondary FlyBase IDs

FBal0261122
FBal0344237
FBal0270136

References (34)

Report Sections

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