FlyBase Gene Report: Dmel\BthD

General Information

Symbol

Dmel\BthD

Species

D. melanogaster

Name

BthD selenoprotein

Annotation Symbol

CG11177

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030501

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

BthD selenoprotein (BthD) encodes a protein involved in salivary gland development and lifespan determination. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

SelH

Key Links

Genomic Location

Cytogenetic map

12A8-12A8

Sequence location

X:13,612,131..13,613,228 [+]

Recombination map

1-44

RefSeq locus

NC_004354 REGION:13612131..13613228

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from direct assay

(Kwon et al., 2003)

involved_in embryo development ending in birth or egg hatching

inferred from direct assay

(Castellano et al., 2001)

involved_in larval salivary gland morphogenesis

inferred from mutant phenotype

(Kwon et al., 2003)

involved_in salivary gland boundary specification

inferred from direct assay

(Kwon et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cell redox homeostasis

traceable author statement

(Castellano et al., 2001)

involved_in response to redox state

non-traceable author statement

(Martin-Romero et al., 2001)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Kwon et al., 2003)

located_in Golgi apparatus

inferred from direct assay

(Kwon et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN002132385

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SELENOPROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

BthD selenoprotein (BthD) encodes a protein involved in salivary gland development and lifespan determination. [Date last reviewed: 2019-09-19]

Gene Group (FlyBase)

SELENOPROTEINS -: Selenoproteins arise from the differential decoding of the UGA stop codon as selenocysteine. Compared with other eukaryotes, arthropods show a reduced complement of selenoproteins. (Adapted from FBrf0216226.)

Protein Function (UniProtKB)

May be involved in a redox-related process. Required for survival and specifically for salivary gland morphogenesis.

(UniProt, Q9VYB0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\BthD for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VYB0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.39

Stop-codon suppression (UGA as Sec) postulated (FBrf0137271).

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

BthD-RA

FBtr0073806

NM_132675

977

249

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

BthD-PA

FBpp0073637

27.8

249

10.44

Q9VYB0

NP_572903

AAF48293

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VYB0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\BthD using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

BthD_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.29

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | intense

Comment: assayed 0-15 hr AEL

(Kwon et al., 2003)

larval stage

organism | faint

(Kwon et al., 2003)

adult stage | female

organism | intense

(Kwon et al., 2003)

adult stage | male

organism | faint

(Kwon et al., 2003)

Additional Descriptive Data

BthD transcript is detected at high levels in embryos from 0-5 hours AEL, and in adult females. BthD is detected at moderate levels from 5-15 hours ALE, and at negligible levels during all three larval instars, and in adult males.

(Kwon et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 4

organism | intense | ubiquitous | uniform

Comment: maternal contribution

(Kwon et al., 2003)

embryonic stage 5 -- 10

organism | faint | ubiquitous

(Kwon et al., 2003)

embryonic stage 14 -- 16

salivary gland body primordium | intense

(Kwon et al., 2003)

oogenesis

ovariole

(Kwon et al., 2003)

oocyte

(Kwon et al., 2003)

Additional Descriptive Data

BthD protein is is detected in ovarioles at all oogenesis stages. It is localized to the cytoplasm of oocytes, and is not detected in follicle or nurse cells. Maternally contributed BthD protein is detected in embryos until cellularization, and is much reduced by embryonic stage 10. BthD protein is detected in the salivary glands at stage 14, accumulating through stage 16.

(Kwon et al., 2003)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Kwon et al., 2003)

located_in Golgi apparatus

inferred from direct assay

(Kwon et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\BthD in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\BthD

View exonic expression by tissue for Dmel\BthD

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32317/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030501

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030501

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030501

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11177

Flygut - An atlas of the Drosophila adult midgut

FBgn0030501

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of BthD

Transgenic constructs containing regulatory region of BthD

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6246

(Ryder, 2004.4.26)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of P-stage, with Scer\GAL4^da.G32

BthD^RNAi.UAS

lethal - all die before end of P-stage, with Scer\GAL4^fkh.PH

BthD^RNAi.UAS

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

BthD^GD6665

partially lethal - majority die, with Scer\GAL4^pnr-MD237

BthD^GD6665

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

BthD^GD6665

Other Phenotypes

Allele

decreased cell size | third instar larval stage, with Scer\GAL4^da.G32

BthD^RNAi.UAS

decreased cell size | third instar larval stage, with Scer\GAL4^fkh.PH

BthD^RNAi.UAS

some die during P-stage, with Scer\GAL4^da.G32

BthD^RNAi.UAS

some die during P-stage, with Scer\GAL4^fkh.PH

BthD^RNAi.UAS

some die during pupal stage, with Scer\GAL4^pnr-MD237

BthD^GD6665

visible, with Scer\GAL4^pnr-MD237

BthD^GD6665

Phenotype manifest in

Allele

embryonic/larval salivary gland, with Scer\GAL4^da.G32

BthD^RNAi.UAS

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

BthD^RNAi.UAS

mesonotum, with Scer\GAL4^pnr-MD237

BthD^GD6665

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\SELENOH

7 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Selenoh

7 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Selenoh

6 of 14

Yes

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (2)

Atha\AT2G24440

3 of 13

Yes

Atha\AT4G31360

3 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:BthD. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

CG13186

4 of 13

CG15147

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SELENOH; selenoprotein H

7 of 14

607914

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

BthD - Sbp2

electrophoretic mobility shift assay, autoradiography

(Takeuchi et al., 2009)

protein-protein

Physical Interaction

Assay

References

BthD - Arc1

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG1532

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG18787

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG18789

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG2540

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG4707

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG5746

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG7049

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Ccs

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Cpr11B

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Fkbp12

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Gmppb

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Got1

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Hpf1

experimental knowledge based

(Guruharsha et al., 2011)

BthD - RpS21

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Wdr82

experimental knowledge based

(Guruharsha et al., 2011)

BthD - knrl

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58694

DroID - A comprehensive database of gene and protein interactions.

FBgn0030501

MIST (protein-protein) - An integrated Molecular Interaction Database

32317

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_stop_codon_redefined_as_selenocysteine

(Martin-Romero et al., 2001)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-44

Cytogenetic map

12A8-12A8

Sequence location

X:13,612,131..13,613,228 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

12A8-12A8

Limits computationally determined from genome sequence between P{EP}CG1640^EP1626 and P{EP}NFAT^EP1353&P{EP}NFAT^EP1508

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-44

(FlyBase, 2016)

1-43.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

43267

y¹ v¹; P{TRiP.GLC01456}attP2

65094

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05991}attP40

VDRC

v45975

w¹¹¹⁸; P{GD6665}v45975/TM3

Genomic Clones (5)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (21)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030501

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030501

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kwon et al., 2003)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Source for identity of BthD CG11177 was sequence comparison ( date:010728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: BthD CG11177

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

BthD

(Xu et al., 2023, Shchedrina et al., 2011, Buffin and Gho, 2010, Kwon et al., 2003)

CG11177

(Hosono et al., 2015, Ni et al., 2010.12.1, Perkins et al., 2009, Haussmann et al., 2008, Dimova et al., 2003, Castellano et al., 2001)

SelH

(Takeuchi et al., 2009, Chapple and Guigó, 2008)

dSelH

(Shchedrina et al., 2011)

dselH

(Morozova et al., 2003)

dselM

(Castellano et al., 2001)

Name Synonyms

BthD selenoprotein

(Buffin and Gho, 2010, Martin-Romero et al., 2001)

BthDselenoprotein

(Zhan et al., 2013)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32317

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VYB0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VYB0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VYB0

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32317/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030501

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030501

FlyMine - An integrated database for Drosophila genomics

FBgn0030501

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11177

MARRVEL_MODEL - MARRVEL (model organism gene)

32317

Linkouts

BioGRID - A database of protein and genetic interactions.

58694

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030501

DroID - A comprehensive database of gene and protein interactions.

FBgn0030501

DRSC - Results frm RNAi screens

FBgn0030501

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

BthD_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030501

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030501

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11177

Flygut - An atlas of the Drosophila adult midgut

FBgn0030501

MIST (protein-protein) - An integrated Molecular Interaction Database

32317

References (38)

The following external sites may use different assemblies or annotations than FlyBase.

-0.29

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