FlyBase Gene Report: Dmel\Fis1

General Information

Symbol

Dmel\Fis1

Species

D. melanogaster

Name

Fission, mitochondrial 1

Annotation Symbol

CG17510

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039969

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Fission, mitochondrial 1 (Fis1) encodes a protein involved in mitochondrial dynamics, more specifically in the mitochondrial fission process. [Date last reviewed: 2023-01-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

41F5-41F5

Sequence location

2R:5,603,587..5,604,834 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:5603587..5604834

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables lipid binding

inferred from biological aspect of ancestor with PANTHER:PTN000326738

(GO Reference Genome Project, 2011-)

enables molecular adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000326738

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in mitochondrion organization

inferred from genetic interaction with FLYBASE:Pink1; FB:FBgn0029891

(Yang et al., 2008)

involved_in positive regulation of mitochondrial fission

inferred from mutant phenotype

(Ziviani et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mitochondrial fission

inferred from electronic annotation with InterPro:IPR016543

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000326738

(GO Reference Genome Project, 2011-)

involved_in peroxisome fission

inferred from biological aspect of ancestor with PANTHER:PTN000326738

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in mitochondrial outer membrane

inferred from biological aspect of ancestor with PANTHER:PTN000326738

(GO Reference Genome Project, 2011-)

is_active_in peroxisomal membrane

inferred from biological aspect of ancestor with PANTHER:PTN000326738

(GO Reference Genome Project, 2011-)

located_in peroxisomal membrane

inferred from sequence or structural similarity with UniProtKB:Q9Y3D6

(Faust et al., 2012)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the FIS1 family. (B7YZT2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Fission, mitochondrial 1 (Fis1) encodes a protein involved in mitochondrial dynamics, more specifically in the mitochondrial fission process. [Date last reviewed: 2023-01-19]

Protein Function (UniProtKB)

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering (PubMed:18443288, PubMed:20194754). Functions downstream of Pink1 and upstream of Drp1 to regulate mitochondrial fission (PubMed:18443288).

(UniProt, B7YZT2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Fis1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry B7YZT2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0086073

630

FBtr0273208

698

154

FBtr0273209

560

FBtr0273210

628

148

FBtr0273211

704

FBtr0345012

557

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Fis1-PA

FBpp0085409

11.0

9.37

FBpp0271716

17.8

154

5.79

FBpp0271717

10.3

8.43

FBpp0271718

17.0

148

5.11

FBpp0271719

10.3

8.43

FBpp0311263

11.0

9.37

Q8SYX2

NP_001286140

AHN55938

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

98 aa isoforms: Fis1-PA, Fis1-PG

92 aa isoforms: Fis1-PD, Fis1-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Domain

The C-terminus is required for mitochondrial localization, while the N-terminus is necessary for mitochondrial fission.

(UniProt, B7YZT2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

B7YZT2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fis1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.15

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fis1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Fis1

View exonic expression by tissue for Dmel\Fis1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039969

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49892/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039969

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039969

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039969

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17510

Flygut - An atlas of the Drosophila adult midgut

FBgn0039969

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Fis1

Transgenic constructs containing regulatory region of Fis1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Phenotype manifest in

Allele

embryonic/larval motor neuron | larval stage, with Scer\GAL4^ple.PF

Fis1^UAS.cLa

indirect flight muscle | adult stage, with Scer\GAL4^Mef2.PR

Fis1^HMS05301

mitochondrion | adult stage, with Scer\GAL4^Mef2.PR

Fis1^HMS05301

mitochondrion | larval stage, with Scer\GAL4^ple.PF

Fis1^UAS.cLa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\FIS1

13 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Fis1

14 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Fis1

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\fis1

7 of 13

Yes

Danio rerio (Zebrafish) (1)

Drer\fis1

13 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\fis-2

12 of 14

Yes

Cele\fis-1

9 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP009852

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\BIGYIN

7 of 13

Yes

Atha\FIS1B

6 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\FIS1

10 of 13

Yes

Scer\GEA1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\fis1

9 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Fis1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

FIS1; fission, mitochondrial 1

13 of 14

609003

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Fis1 - Drp1

anti tag coimmunoprecipitation, anti tag western blot

(Ti et al., 2025)

Fis1 - Rab14

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72485

DroID - A comprehensive database of gene and protein interactions.

FBgn0039969

MIST (protein-protein) - An integrated Molecular Interaction Database

49892

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Mitophagy - animal

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class I peroxisomal membrane protein import

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

41F5-41F5

Sequence location

2R:5,603,587..5,604,834 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

41F5-41F5

Limits computationally determined from genome sequence between P{PZ}vlc⁰⁷⁰²²&P{lacW}l(2)09851^k08138 and P{PZ}l(2)09851⁰⁹⁸⁵¹&P{lacW}Src42A^k10108

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

55496

y¹ w^*; Mi{MIC}Fis1^MI10520

92213

y¹ w^*; TI{CRIMIC.TG4.0}Fis1^{CR02335-TG4.0}/SM6a

604614

y¹ sc^* v¹ sev²¹; P{TOE.GS00316}attP40

63027

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05301}attP40

76480

y¹ sc^* v¹ sev²¹; P{TKO.GS00928}attP40/CyO

VDRC

v330491

P{VSH330491}attP40

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (22)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE29957

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039969

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE20116

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039969

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2021, Chen et al., 2020)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG17510 NEST:bs15a08

(FlyBase Genome Annotators, 2005)

Source for merge of: CG17510 BcDNA:RE29957

(FlyBase, 1996-)

Additional comments

Source for merge of CG17510 BcDNA:RE29957 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

BcDNA:RE29957

CG17510

(Meadows, 2018.4.4, Baron et al., 2016, Caizzi et al., 2016, Debattisti and Scorrano, 2013, Faust et al., 2012, Perkins et al., 2009, Rossi et al., 2007)

FIS1

(Anderson-Baron and Simmonds, 2018, Baron et al., 2016, Mendez et al., 2016)

Fis1

(Bosch et al., 2023, Katti et al., 2022, Lee et al., 2021, Chen et al., 2020, Funikov et al., 2020, Ponte et al., 2020, Pridie et al., 2020, Meltzer et al., 2019, Anderson-Baron and Simmonds, 2018, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, Debattisti and Scorrano, 2013, Kwon et al., 2013, Faust et al., 2012, Liu et al., 2012, Ziviani et al., 2010)

NEST:bs15a08

bs15a08.y1

(Andrews et al., 2000)

dFis1

(Yang et al., 2008)

fis1

(Li et al., 2023, Liu and Bossing, 2016, Mendez et al., 2016)

Name Synonyms

Fission, mitochondrial 1

(Anderson-Baron and Simmonds, 2018)

Secondary FlyBase IDs

FBgn0046054
FBgn0047128

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

49892

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

B7YZT2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

B7YZT2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

B7YZT2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039969

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49892/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039969

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039969

FlyMine - An integrated database for Drosophila genomics

FBgn0039969

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17510

MARRVEL_MODEL - MARRVEL (model organism gene)

49892

Linkouts

BioGRID - A database of protein and genetic interactions.

72485

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039969

DroID - A comprehensive database of gene and protein interactions.

FBgn0039969

DRSC - Results frm RNAi screens

FBgn0039969

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039969

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039969

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17510

Flygut - An atlas of the Drosophila adult midgut

FBgn0039969

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0039969

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014659

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Mitophagy - animal

MIST (protein-protein) - An integrated Molecular Interaction Database

49892

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class I peroxisomal membrane protein import

References (54)

The following external sites may use different assemblies or annotations than FlyBase.

-1.15

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