FB2026_02 , released June 18, 2026
Gene: Dmel\Boh1
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General Information
Symbol
Dmel\Boh1
Species
D. melanogaster
Name
Buddy of Hmr 1
Annotation Symbol
CG33213
Feature Type
protein_coding_gene
FlyBase ID
FBgn0053213
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
98B6-98B6
Sequence location
Recombination map
3-94
RefSeq locus
NT_033777 REGION:27918182..27919460
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor activity, RNA polymerase II-specific
inferred from biological aspect of ancestor with PANTHER:PTN000147941
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P32338
(Gene Ontology Curators, 2002-)
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding
inferred from sequence or structural similarity with UniProtKB:P32338
(Gene Ontology Curators, 2002-)
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000147941
(GO Reference Genome Project, 2011-)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000147941
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P32338
(Gene Ontology Curators, 2002-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in pericentric heterochromatin
inferred from direct assay
(Lukacs et al., 2021)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000147941
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from sequence or structural similarity with UniProtKB:P32338
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Boh1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VAY8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.53

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Boh1-RA
FBtr0085271
NM_176572
1279
341
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Boh1-PA
FBpp0084640
38.7
341
10.16
Q9VAY8
NP_788749
AAO41605
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Boh1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.28

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in pericentric heterochromatin
inferred from direct assay
(Lukacs et al., 2021)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Boh1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Boh1
Transgenic constructs containing regulatory region of Boh1
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
    Variants
    Variant Molecular Consequences
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Homo sapiens (Human) (40)
    Hsap\IKZF2
    1 of 14
    Yes
    No
    Hsap\PLAGL2
    1 of 14
    Yes
    Yes
    Hsap\ZBTB24
    1 of 14
    Yes
    No
    1  
    Hsap\ZFP28
    1 of 14
    Yes
    No
    Hsap\ZFP69B
    1 of 14
    Yes
    No
    Hsap\ZFP90
    1 of 14
    Yes
    No
    Hsap\ZKSCAN2
    1 of 14
    Yes
    No
    1  
    Hsap\ZKSCAN7
    1 of 14
    Yes
    No
    Hsap\ZNF16
    1 of 14
    Yes
    No
    Hsap\ZNF23
    1 of 14
    Yes
    No
    Hsap\ZNF26
    1 of 14
    Yes
    No
    Hsap\ZNF44
    1 of 14
    Yes
    No
    Hsap\ZNF70
    1 of 14
    Yes
    No
    Hsap\ZNF84
    1 of 14
    Yes
    No
    Hsap\ZNF157
    1 of 14
    Yes
    No
    Hsap\ZNF169
    1 of 14
    Yes
    No
    Hsap\ZNF182
    1 of 14
    Yes
    No
    Hsap\ZNF211
    1 of 14
    Yes
    No
    Hsap\ZNF263
    1 of 14
    Yes
    No
    Hsap\ZNF268
    1 of 14
    Yes
    No
    Hsap\ZNF287
    1 of 14
    Yes
    No
    Hsap\ZNF316
    1 of 14
    Yes
    No
    Hsap\ZNF333
    1 of 14
    Yes
    Yes
    Hsap\ZNF358
    1 of 14
    Yes
    No
    Hsap\ZNF394
    1 of 14
    Yes
    No
    Hsap\ZNF441
    1 of 14
    Yes
    No
    Hsap\ZNF544
    1 of 14
    Yes
    No
    Hsap\ZNF572
    1 of 14
    Yes
    No
    Hsap\ZNF629
    1 of 14
    Yes
    No
    Hsap\ZNF700
    1 of 14
    Yes
    No
    Hsap\ZNF764
    1 of 14
    Yes
    No
    Hsap\ZNF768
    1 of 14
    Yes
    No
    Hsap\ZNF772
    1 of 14
    Yes
    No
    Hsap\ZNF823
    1 of 14
    Yes
    No
    Hsap\ZNF835
    1 of 14
    Yes
    No
    Hsap\ZNF836
    1 of 14
    Yes
    No
    Hsap\ZNF875
    1 of 14
    Yes
    No
    Hsap\ZNF878
    1 of 14
    Yes
    No
    Hsap\ZSCAN20
    1 of 14
    Yes
    No
    Hsap\ZSCAN25
    1 of 14
    Yes
    No
    Model Organism Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Rattus norvegicus (Norway rat) (4)
    Rnor\LOC501406
    1 of 14
    Yes
    No
    Rnor\Zbtb24
    1 of 14
    Yes
    Yes
    Rnor\Zfp287
    1 of 14
    Yes
    No
    Rnor\Zfp358
    1 of 14
    Yes
    Yes
    Mus musculus (laboratory mouse) (9)
    Mmus\Zbtb24
    1 of 14
    Yes
    Yes
    Mmus\Zfp59
    1 of 14
    Yes
    No
    Mmus\Zfp90
    1 of 14
    Yes
    No
    Mmus\Zfp273
    1 of 14
    Yes
    No
    Mmus\Zfp287
    1 of 14
    Yes
    Yes
    Mmus\Zfp358
    1 of 14
    Yes
    No
    Mmus\Zfp629
    1 of 14
    Yes
    No
    Mmus\Zfp748
    1 of 14
    Yes
    No
    Mmus\Zfp949
    1 of 14
    Yes
    No
    Xenopus tropicalis (Western clawed frog) (4)
    Xtro\LOC100493522
    1 of 13
    Yes
    No
    Xtro\LOC100498654
    1 of 13
    Yes
    Yes
    Xtro\LOC108645295
    1 of 13
    Yes
    No
    Xtro\zfp2
    1 of 13
    Yes
    No
    Danio rerio (Zebrafish) (4)
    Drer\si:ch73-138e16.4
    1 of 14
    Yes
    No
    Drer\si:dkey-34m19.3
    1 of 14
    Yes
    No
    Drer\wu:fe05a04
    1 of 14
    Yes
    Yes
    Drer\zgc:113343
    1 of 14
    Yes
    No
    Caenorhabditis elegans (Nematode, roundworm) (1)
    Cele\zfp-2
    1 of 14
    Yes
    No
    Anopheles gambiae (African malaria mosquito) (3)
    Agam\AgaP_AGAP002705
    1 of 12
    Yes
    No
    Agam\AgaP_AGAP004331
    1 of 12
    Yes
    No
    Agam\AgaP_AGAP006758
    1 of 12
    Yes
    No
    Arabidopsis thaliana (thale-cress) (0)
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    Schizosaccharomyces pombe (Fission yeast) (0)
    Escherichia coli (enterobacterium) (0)
    Other Organism Orthologs (via OrthoDB)
    Data provided directly from OrthoDB:Boh1. Refer to their site for version information.
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v9.1)
    Gene Symbol
    Score
    Source
    Compara
    Domainoid
    eggNOG
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Drosophila melanogaster (Fruit fly) (4)
    CG6654
    1 of 13
    gl
    1 of 13
    Phs
    1 of 13
    rgr
    1 of 13
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-94
      Cytogenetic map
      98B6-98B6
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      98B6-98B6
      Limits computationally determined from genome sequence between P{PZ}btzrL203 and P{PZ}l(3)0648706487
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-94

      (FlyBase, 2016)

      3-92.4

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (10)
      Genomic Clones (15)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (49)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
        Other Comments
        Relationship to Other Genes
        Source for database merge of
        Additional comments

        "CG33221" resembles a retrotransposed, intronless copy of CG33213.

        (Hogan and Bettencourt, 2009)

        The CR33221 and CG33213 genes share at least 50% identity.

        (Langille and Clark, 2007)

        FlyBase curator comment: The symbol "CG12260" has been used erroneously in FlyBase to name 2 separate annotations (one on chromosome X, the other on arm 3R) in different releases of the genome annotation. The two annotations have been renamed to avoid confusion; they are CG33221 (on the X) and CG33213 (on 3R). CG33213 was labelled "CG12260" in releases 1 and 2, was not annotated in release 3.0 and was labelled "CG33213" in 3.1 of the genome annotation. CG33221 was a new annotation in release 3.1 of the genome annotation, when it was mis-labelled "CG12260".

        (FlyBase Genome Annotators, 2004)
        Nomenclature History
        Source for database identify of

        Source for identity of: Boh1 CG33213

        (Lukacs et al., 2021)
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (4)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 30 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        MIST (protein-protein) - An integrated Molecular Interaction Database
        References (36)