FlyBase Gene Report: Dmel\spg

General Information

Symbol

Dmel\spg

Species

D. melanogaster

Name

sponge

Annotation Symbol

CG31048

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264324

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

sponge (spg) encodes a DOCK family RhoGEF involved in nervous system development and regulation of signaling pathways. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Red Book
Interactive Fly

Also Known As

mat(3)6

Key Links

Genomic Location

Cytogenetic map

98E6-98F1

Sequence location

3R:28,845,270..28,870,439 [+]

Recombination map

3-97

RefSeq locus

NT_033777 REGION:28845270..28870439

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from electronic annotation with InterPro:IPR026791

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:mbc; FB:FBgn0015513

(Meller et al., 2005)

inferred from biological aspect of ancestor with PANTHER:PTN002902098

(GO Reference Genome Project, 2011-)

enables small GTPase binding

inferred from biological aspect of ancestor with PANTHER:PTN002902098

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in central nervous system development

inferred from mutant phenotype

(Biersmith et al., 2011)

involved_in positive regulation of ERK1 and ERK2 cascade

inferred from mutant phenotype

(Eguchi et al., 2013)

involved_in positive regulation of sevenless signaling pathway

inferred from mutant phenotype

(Eguchi et al., 2013)

involved_in R7 cell fate commitment

inferred from mutant phenotype

(Eguchi et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in small GTPase-mediated signal transduction

inferred from electronic annotation with InterPro:IPR026791

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Biersmith et al., 2011)

located_in cytosol

inferred from direct assay

(Biersmith et al., 2011)

located_in plasma membrane

inferred from direct assay

(Biersmith et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002902098

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002902098

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS

Pathway (FlyBase)

POSITIVE REGULATORS OF SEVENLESS SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

sponge (spg) encodes a DOCK family RhoGEF involved in nervous system development and regulation of signaling pathways. [Date last reviewed: 2019-08-01]

Gene Group (FlyBase)

RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS -: RhoGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Rho family GTPases. They include Dbl-homology domain-containing proteins (the largest family of RhoGEFs) and CZH2 domain/Dock family GEFs. (Adapted from FBrf0190957).

Pathway (FlyBase)

POSITIVE REGULATORS OF SEVENLESS SIGNALING PATHWAY -: Positive regulators of Sevenless signaling up-regulate the pathway, promoting cell fate to that of an R7 photoreceptor. (Adapted from FBrf0127283 and FBrf0221727).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

spg: sponge

Maternal-effect lethal; embryos produced by homozygous females produce pole cells, but syncytial blastoderm fails to cellularize completely; cells form at the anterior and posterior ends of the embryo but are separated by a non-cellular region comprising 70% of the embryo surface. Abortive gastrulation signified by formation of posterior midgut rudiment.

Summary (Interactive Fly)

member of the Dedicator of cytokinesis (DOCK) scaffolding family - regulates actin filament polymerization and/or depolymerization and are GEF proteins, which contribute to cellular signaling events by activating small G proteins - a Drosophila counterpart to mammalian DOCK3/DOCK4 - plays a role in embryonic cellularization, central nervous system development, R7 photoreceptor cell differentiation, and adult thorax development

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\spg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VAS8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 6.02

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

spg-RD

FBtr0330303

NM_001144646

6722

2002

spg-RE

FBtr0330304

NM_170379

7513

2187

spg-RF

FBtr0330305

NM_001276118

6710

2008

spg-RG

FBtr0336765

NM_001276119

7249

2008

spg-RH

FBtr0336766

NM_001276120

8052

2002

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

spg-PD

FBpp0303335

227.0

2002

6.85

FBpp0303336

247.3

2187

6.78

FBpp0303337

227.7

2008

6.83

FBpp0307741

227.7

2008

6.83

FBpp0307742

227.0

2002

6.85

B7Z0R2

NP_001263049

AGB96429

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2002 aa isoforms: spg-PD, spg-PH

2008 aa isoforms: spg-PF, spg-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\spg using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

spg_1
spg_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.03

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

presumptive embryonic/larval central nervous system

(Biersmith et al., 2011)

presumptive embryonic/larval peripheral nervous system

(Biersmith et al., 2011)

embryonic stage 13

embryonic somatic muscle cell

(Biersmith et al., 2011)

embryonic visceral muscle cell

(Biersmith et al., 2011)

embryonic stage 13 -- 17

larval ventral nerve cord

(Biersmith et al., 2011)

embryonic stage 16

embryonic/larval dorsal vessel

(Biersmith et al., 2011)

larval brain

(Biersmith et al., 2011)

Additional Descriptive Data

spg is expressed strongly in the developing nervous system throughout embryonic development. Expression is also detected in somatic and visceral musculature and in the dorsal vessel.

(Biersmith et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13

embryonic visceral muscle cell

(Biersmith et al., 2011)

embryonic stage 13 -- 17

larval ventral nerve cord

(Biersmith et al., 2011)

symmetrical commissure

(Biersmith et al., 2011)

larval longitudinal connective

(Biersmith et al., 2011)

third instar larval stage

mesonotum

(Binh et al., 2019)

wing disc dorsal compartment

(Morishita et al., 2014)

wandering third instar larval stage

eye disc posterior to the morphogenetic furrow

(Eguchi et al., 2013)

photoreceptor cell R7

(Eguchi et al., 2013)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13

larval peripheral nervous system

(Biersmith et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

spg protein is detected in the eye disc immediately posterior to the morphogenetic furrow, with signal becoming stronger more posterior in the disc. This expression is predominantly in R7 photoreceptor cells, with low levels expression detected in other photoreceptor cells.

(Eguchi et al., 2013)

spg protein is detected in the ventral nerve cord and in visceral muscle. It is also detected in all commissural and longitudinal neurons. It is not detected in glial cells.

(Biersmith et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Biersmith et al., 2011)

located_in cytosol

inferred from direct assay

(Biersmith et al., 2011)

located_in plasma membrane

inferred from direct assay

(Biersmith et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\spg in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\spg

View exonic expression by tissue for Dmel\spg

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43404/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264324

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051048

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264324

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of spg

Transgenic constructs containing regulatory region of spg

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)3450

(Hayashi et al., 1997)

Df(3R)Exel6210

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

spg^GD10145

lethal - all die during embryonic stage, with Scer\GAL4^Act5C.PU

spg^{RNAi.1447-1632.UAS}

lethal - all die during embryonic stage, with Scer\GAL4^en.PU

spg^{RNAi.1447-1632.UAS}

lethal - all die during embryonic stage, with Scer\GAL4^Tub.PU

spg^{RNAi.1447-1632.UAS}

lethal - all die during embryonic stage | maternal effect

lethal - all die during embryonic stage | maternal effect | recessive

lethal - all die during embryonic stage | non-rescuable maternal effect | recessive

spg^unspecified

lethal - all die during pupal stage, with Scer\GAL4^Ddc.PL

spg^{RNAi.1447-1632.UAS}

lethal - all die during pupal stage, with Scer\GAL4^MsrA.657

spg^{RNAi.1447-1632.UAS}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

spg^GD10145

viable, with Scer\GAL4^tin.CΔ4

spg^GD10145

Other Phenotypes

Allele

abnormal neuroanatomy | embryonic stage

spg²

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav-C155

spg^GD10145

abnormal neuroanatomy | embryonic stage (with Df(3R)3450)

abnormal neuroanatomy | embryonic stage (with spg²)

spg³

abnormal neuroanatomy | embryonic stage (with spg³)

spg²

decreased cell number | embryonic stage | maternal effect

spg^unspecified

some die during embryonic stage | maternal effect

some die during embryonic stage | recessive

spg^unspecified

some die during embryonic stage | recessive | maternal effect | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

spg^GD10145

visible, with Scer\GAL4³⁸⁹

spg^{RNAi.1447-1632.UAS}

visible, with Scer\GAL4^GMR.PU

visible, with Scer\GAL4^pnr-MD237

spg^{RNAi.1447-1632.UAS}

Phenotype manifest in

Allele

adult thorax, with Scer\GAL4^pnr-MD237

spg^{RNAi.1447-1632.UAS}

amnioproctodeal invagination | maternal effect

spg^unspecified

embryo | blastoderm stage | maternal effect

spg^unspecified

embryo | embryonic stage 4 | maternal effect

spg^unspecified

embryonic cortex | cleavage stage

spg^unspecified

eye, with Scer\GAL4^GMR.PU

lateral longitudinal fascicle

spg²

lateral longitudinal fascicle, with Scer\GAL4^elav-C155

spg^GD10145

lateral longitudinal fascicle (with Df(3R)3450)

lateral longitudinal fascicle (with spg²)

spg³

lateral longitudinal fascicle (with spg³)

spg²

nucleus | embryonic stage 4 | maternal effect

spg^unspecified

ommatidium, with Scer\GAL4^GMR.PU

spg^{RNAi.1447-1632.UAS}

primordial germ cell | maternal effect

spg^unspecified

wing, with Scer\GAL4³⁸⁹

spg^{RNAi.1447-1632.UAS}

yolk | maternal effect

spg^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\DOCK3

12 of 14

Yes

Hsap\DOCK4

11 of 14

Yes

8 of 14

8 of 14

7 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (10)

Rnor\Dock3

12 of 14

Yes

Rnor\Dock4

10 of 14

Yes

8 of 14

7 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Dock3

12 of 14

Yes

Mmus\Dock4

11 of 14

Yes

8 of 14

8 of 14

7 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\dock3

8 of 13

Yes

Xtro\dock4

6 of 13

Yes

6 of 13

3 of 13

3 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (14)

Drer\dock3

12 of 14

Yes

Drer\dock4b

11 of 14

Yes

Drer\dock4

9 of 14

Yes

Drer\dock5

8 of 14

Drer\dock1

7 of 14

Drer\dock2

3 of 14

Yes

Drer\dock2-like

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\ced-5

11 of 14

Yes

Cele\F46H5.4

2 of 14

Cele\F22G12.5

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP004507

11 of 12

Yes

6 of 12

2 of 12

2 of 12

2 of 12

Arabidopsis thaliana (thale-cress) (2)

Atha\SPK1

2 of 13

Yes

Atha\AT4G08360

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\DCK1

8 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:spg. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

mbc

10 of 13

Zir

2 of 13

Ziz

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

spg^GD10145

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DOCK3; dedicator of cytokinesis 3

12 of 14

603123

NEURODEVELOPMENTAL DISORDER WITH IMPAIRED INTELLECTUAL DEVELOPMENT, HYPOTONIA, AND ATAXIA; NEDIDHA

DOCK4; dedicator of cytokinesis 4

11 of 14

607679

DOCK1; dedicator of cytokinesis 1

8 of 14

601403

DOCK5; dedicator of cytokinesis 5

8 of 14

616904

DOCK2; dedicator of cytokinesis 2

7 of 14

603122

IMMUNODEFICIENCY 40; IMD40

immunodeficiency 40

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

spg - Ced-12

anti tag coimmunoprecipitation, western blot, multidimensional protein identification technology

(Biersmith et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

spg

enhanceable

Ced-12

(Biersmith et al., 2011)

spg

suppressible

Raf

(Eguchi et al., 2013)

spg

suppressible

sev

(Eguchi et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ced-12

enhanceable

spg

(Biersmith et al., 2011)

mbc

enhanceable

spg

(Biersmith et al., 2011)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264324

MIST (protein-protein) - An integrated Molecular Interaction Database

43404

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF SEVENLESS SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-97

Cytogenetic map

98E6-98F1

Sequence location

3R:28,845,270..28,870,439 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98E6-98F1

Limits computationally determined from genome sequence between P{PZ}l(3)06487⁰⁶⁴⁸⁷ and P{EP}EP3390^EP3390

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-97

(FlyBase, 2016)

3-94.3

(Comeron et al., 2012)

3-95

(Tearle and Nusslein-Volhard, 1987)

3-98.1

(Rice and Garen, 1975)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

35396

y¹ v¹; P{TRiP.GL00316}attP2

52195

y¹ w^*; Mi{MIC}spg^MI09702

9039

mwh¹ red¹ e¹ spg⁵/TM1

76205

y¹ w^*; Mi{Trojan-GAL4.1}spg^{MI09702-TG4.1}

34367

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01356}attP2/TM3, Sb¹

NIG-Fly

GL00316

y¹ sc^* v¹ sev²¹; P{TRiP.GL00316}attP2

VDRC

v21293

w¹¹¹⁸ P{GD10145}v21293

v47316

w¹¹¹⁸; P{GD10145}v47316/TM3

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Eguchi et al., 2013, Biersmith et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

The sponge gene product is apparently required for the formation of actin caps over interphase nuclei at the syncytial blastoderm and for the formation of metaphase pseudocleavage furrows.

(Postner et al., 1992)

spg function is essential only for oogenesis and not for any other period of development.

(Rice and Garen, 1975)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG11754 CG14530

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: spg CG31048

(Biersmith et al., 2011)

Additional comments

Annotations CG11754 and CG14530 merged as CG31048 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG11754

CG14530

CG31048

(Çiçek et al., 2016, Eguchi et al., 2013, Butchar et al., 2012, Christis and Munro, 2012, Biersmith et al., 2011, Friedman et al., 2011, Jungreis et al., 2011, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Stuart et al., 2007)

Dm Dock4

(Meller et al., 2005)

Spg

(Campanale et al., 2022, Biersmith et al., 2015)

Sponge

(Lv et al., 2021)

mat(3)6

(Costa et al., 1993, Schweisguth et al., 1991, Warn, 1990, Rickoll and Counce, 1981, Rice and Garen, 1975)

mat3(6)

Name Synonyms

Dock4

(Balagopalan et al., 2006)

Sponge

(Hudson et al., 2019, Schmidt and Grosshans, 2018, Biersmith et al., 2015, Balagopalan et al., 2006)

early-D

sponge

(Hudson et al., 2021, Lv et al., 2020, Schmidt et al., 2018, Morishita et al., 2014, Eguchi et al., 2013, Biersmith et al., 2011, Haralalka et al., 2011, Cao et al., 2010, Riggs et al., 2007, Riparbelli et al., 2007, Schejter et al., 1997)

Secondary FlyBase IDs

FBgn0003472
FBgn0051048
FBgn0039605
FBgn0039607

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43404

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VAS8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VAS8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43404/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264324

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264324

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31048

MARRVEL_MODEL - MARRVEL (model organism gene)

43404

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264324

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

spg_1
spg_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051048

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0264324

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0264324

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034453

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

43404

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (101)

The following external sites may use different assemblies or annotations than FlyBase.

-1.03

Report Sections