FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, wts
FlyBase ID
FBig0000098036
Interaction Network
Interactions Browser links
yki network
wts network
Reported Interactions
FBrf0187228-1.Y2H
Reference
Huang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

bait

fused to hSos GDP-GTP exchange factor

wts 
yki 

CG4005

prey

fused to myristoylation signal

yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wts 
N-terminal region
sufficient binding region
aa 1-608
yki 
C-terminal region
sufficient binding region
aa 229-418
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast Cytotrap. Interaction of bait and prey proteins in the cytoplasm restores the SOS/cdc25 pathway in yeast, allowing survival at elevated temperatures.

(Huang et al., 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Huang et al., 2005)

Three wts-interacting clones corresponded to yki.

(Huang et al., 2005)
FBrf0187228-2.coIP.WB
Reference
Huang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

yki 
wts 

Wts

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
mutation disrupting interaction
aa 292-364

W292A, P295A, W261A, P364A \'WM\' mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2005)
FBrf0187228-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

enzyme

V5 tag

wts 

Wts

enzyme

V5 tag

wts 
yki 

Yki

enzyme target

GST tag, [32]P label

yki 

Yki

enzyme target

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0202397-2.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

enzyme

V5 tag

wts 
wts 

Wts

enzyme

V5 tag

yki 

Yki

enzyme target
yki 
yki 

Yki

enzyme target
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0204632-3.coIP.WB
Reference
Wu et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

wts 

Wts

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wu et al., 2008)

Mutations to the yki WW domain(s) abolish binding to wts (exact mutations tested not reported).

(Wu et al., 2008)
FBrf0207465-7.coIP.WB
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

wts 

Wts

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-transfection of ex inhibits the yki-wts interaction.

(Badouel et al., 2009)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Badouel et al., 2009)
FBrf0208409-1.coIP.WB
Reference
Zhang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

yki 
yki 
yki 
wts 

Wts

prey

V5 tag

wts 
wts 
wts 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW1 domain
mutation decreasing interaction
unspecified

described as mutation of second conserved W to F and conserved P to A in WW1

yki 
WW2 domain
mutation decreasing interaction
unspecified

described as mutation of second conserved W to F and conserved P to A in WW2

yki 
WW1 and WW2 domain
mutation disrupting interaction
unspecified

described as mutation of second conserved W to F and conserved P to A in both WW domains

wts 
PY1 and PY2 domain
mutation decreasing interaction
unspecified

described as mutation of conserved Y to A in PPxY motifs 1 and 2

wts 
PY2 and PY4 domain
mutation decreasing interaction
unspecified

described as mutation of conserved Y to A in PPxY motifs 2 and 4

wts 
PY1,PY3 and PY4 domain
mutation decreasing interaction
unspecified

described as mutation of conserved Y to A in PPxY motifs 1, 3 and 4

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2009)
FBrf0208409-2.coIP.WB
Reference
Zhang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
eye disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

bait

Myc tag

yki 

Yki

prey
yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW1 and WW2 domain
mutation disrupting interaction
unspecified

described as mutation of second conserved W to F and conserved P to A in both WW domains

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was eye discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Zhang et al., 2009)
FBrf0215087-3.coIP.WB
Reference
Oh et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

V5 tag

yki 
wts 

Wts

prey

Myc tag

wts 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
mutation disrupting interaction
wts 
PPXY motifs
mutation disrupting interaction
aa 286-550

Y286A, Y304A, Y410A, Y463A, Y550A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh et al., 2009)
FBrf0220780-2.coIP.WB
Reference
Sansores-Garcia et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

wts 

Wts

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sansores-Garcia et al., 2013)

Positive control.

(Sansores-Garcia et al., 2013)
FBrf0224345-175.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
wts 

wts

bait

Protein A tag, Strep tag

yki 

yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0224345-204.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

wts 

wts

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0226836-5.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wts 

wts

enzyme

V5 tag

wts 

wts

enzyme

V5 tag

yki 

yki

enzyme target

GST tag

yki 

yki

enzyme target

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0232944-1.PD.MS
Reference
Hu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

GST tag

yki 
wts 

Wts

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Hu et al., 2016)
FBrf0244666-3.coIP.WB
Reference
Hao et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ATCC
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

bait

Myc tag

yki 

Yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hao et al., 2020)
FBrf0247927-10.coIP.WB
Reference
Srivastava et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

wts 

Wts

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Yki2/yki-PF isoform including exon 3 interacted more strongly with wts than an isoform lacking exon 3.

(Srivastava et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Srivastava et al., 2021)
FBrf0255981-5.coIP.WB
Reference
Zhao et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

wts 

Wts

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhao et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (13)