AGO1, mir-ban Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

TAP tag

mir-ban

ban

prey

mir-ban

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Okamura et al., 2004)

AGO1 associates with both the precursor and mature forms of ban miRNA.

(Okamura et al., 2004)

Mature ban miRNA coimmunoprecipitates with AGO1, AGO2 and Fmr1 in this assay. By contrast, the ban pre-miRNA coimmunoprecipitates only with AGO1.

(Okamura et al., 2004)

FBrf0190554-1.coIP.NB

Reference

Miyoshi et al., 2005

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

mir-ban

ban

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

AGO1 but not AGO2 coimmunoprecipitates ban miRNA.

(Miyoshi et al., 2005)

Interaction in vitro; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2005)

FBrf0191204-5.coIP.NB

Reference

Rehwinkel et al., 2006

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

HA tag

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

ban (mature miRNA and pre-miRNA) coimmunoprecipitates with AGO1, and to a lesser extent, with AGO2 as well.

(Rehwinkel et al., 2006)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rehwinkel et al., 2006)

FBrf0200521-2.coIP.NB

Reference

Förstemann et al., 2007

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The majority of mature ban miRNA, but not ban pre-miRNA, coimmunoprecipitates with AGO1.

(Förstemann et al., 2007)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Förstemann et al., 2007)

FBrf0204460-2.coIP.NB

Reference

Eulalio et al., 2008

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

HA tag

AGO1

mir-ban

Bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

central region

mutation disrupting interaction

aa 594-629

F594V, F629V \'F2V2\' mutant, coordinates relative to 984 residue isoform

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2008)

FBrf0206362-34.coIP.PCR

Reference

Zhou et al., 2008

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

Ago1

bait

FLAG tag

mir-ban

Bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zhou et al., 2008)

FBrf0207830-1.coIP.NB

Reference

Flynt et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2R+

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Flynt et al., 2009)

Positive control.

(Flynt et al., 2009)

FBrf0215456-13.coIP.WB

Reference

Miyoshi et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

FLAG tag

AGO1

mir-ban

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

Mid domain

mutation disrupting interaction

aa 560-595

F560V, F595V \'F2V2\' mutant, coordinates relative to 950 residue isoform

AGO1

PAZ domain

mutation disrupting interaction

aa 369-391

F369A, F387A, Y386A, Y391A, coordinates relative to 950 residue isoform

AGO1

piwi domain

necessary binding region

aa 691-950

coordinates relative to 950 residue isoform

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215456-8.coIP.NB

Reference

Miyoshi et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Anti-AGO1 immunoprecipitates mature ban miRNA almost exclusively, and not ban pre-miRNA.

(Miyoshi et al., 2009)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215487-3.coIP.NB

Reference

Eulalio et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

HA tag

AGO1

mir-ban

Bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

interaction domain residues 594,629

mutation disrupting interaction

aa 594,629

F594V,F629V, coordinates relative to AGO1-PA

AGO1

PAZ domain residues 386-388,446-448

mutation disrupting interaction

aa 386-388,446-448

R386A,K387A,Y388A,T446A,Y447A,L448A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 716,777

mutation disrupting interaction

aa 716,777

I716A,F777A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 785

mutation decreasing interaction

aa 785

K785A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 707

mutation decreasing interaction

aa 707

R707A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 749

mutation decreasing interaction

aa 749

Y749A, coordinates relative to AGO1-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2009)

FBrf0220921-3.coIP.NB

Reference

Nishida et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

Ago1

bait

FLAG tag

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

In wild-type cells, AGO1 binds miRNA, and AGO2 binds siRNA. Upon r2d2 knockdown, AGO1 inappropriately binds siRNA.

(Nishida et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Nishida et al., 2013)

FBrf0221629-1.FB.A

Reference

Hur et al., 2013

Description

physical association

Assay

filter binding

competition binding

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

DmAgo1

bait

MBP tag

AGO1

mir-ban

bantam

prey

[32]P label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

C-terminal domain

sufficient binding region

aa 538-950

coordinates relative to AGO1-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized and labeled in vitro.

(Hur et al., 2013)

FBrf0221629-3.FP.A

Reference

Hur et al., 2013

Description

physical association

Assay

nuclease footprinting

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

DmAgo1

bait

MBP tag

AGO1

mir-ban

bantam

prey

[32]P label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

N-terminal domain

mutation decreasing interaction

aa 1-538

coordinates relative to AGO1-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized and labeled in vitro.

(Hur et al., 2013)

Deletion of the AGO1 N-terminus decreases its protection of the ban 3\' end.

(Hur et al., 2013)

FBrf0221629-4.coIP.NB

Reference

Hur et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

DmAgo1

bait

FLAG tag

AGO1

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

N-terminal domain

necessary binding region

aa 1-538

coordinates relative to AGO1-PB

AGO1

N-terminal domain

mutation disrupting interaction

aa 352,353,354,412,413,414

R352A,K353A,Y354A,T412A,Y413A,L414A, coordinates relative to AGO1-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hur et al., 2013)

FBrf0221629-5.coIP.FP.A

Reference

Hur et al., 2013

Description

physical association

Assay

nuclease footprinting

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

DmAgo1

bait

FLAG tag

AGO1

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

N-terminal domain

mutation disrupting interaction

aa 619,623

Y619L,K623E, coordinates relative to AGO1-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hur et al., 2013)

RNA immunoprecipitated with AGO1 was hybridized to a labeled ban RNA probe and digested with a single strand specific RNase revealing full length and partially degraded ban bound to AGO1. N-terminally deleted or mutated AGO1 bound only the shorter partially degraded ban and to a lesser extent than wildtype.

(Hur et al., 2013)

FBrf0222591-1.coIP.NB

Reference

Carré et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

Ago1

bait

mir-ban

bantam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Carré et al., 2013)

FBrf0224072-4.coIP.NB

Reference

Yang et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2R+

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

FLAG tag

mir-ban

ban

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yang et al., 2014)

FBrf0227265-1.coIP.NB

Reference

Wen et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used