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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098206
Interaction Network
Interactions Browser links
AGO1 network
mir-ban network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0179363-4.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

TAP tag

ban

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

AGO1 associates with both the precursor and mature forms of ban miRNA.

Mature ban miRNA coimmunoprecipitates with AGO1, AGO2 and Fmr1 in this assay. By contrast, the ban pre-miRNA coimmunoprecipitates only with AGO1.

FBrf0190554-1.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

ban

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

AGO1 but not AGO2 coimmunoprecipitates ban miRNA.

Interaction in vitro; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0191204-5.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ban (mature miRNA and pre-miRNA) coimmunoprecipitates with AGO1, and to a lesser extent, with AGO2 as well.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0200521-2.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The majority of mature ban miRNA, but not ban pre-miRNA, coimmunoprecipitates with AGO1.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0204460-2.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

AGO1 

Bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
central region
mutation disrupting interaction
aa 594-629

F594V, F629V \'F2V2\' mutant, coordinates relative to 984 residue isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0206362-34.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

Bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0207830-1.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

FBrf0215456-13.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

FLAG tag

AGO1 
AGO1 
AGO1 

GW182

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
Mid domain
mutation disrupting interaction
aa 560-595

F560V, F595V \'F2V2\' mutant, coordinates relative to 950 residue isoform

AGO1 
PAZ domain
mutation disrupting interaction
aa 369-391

F369A, F387A, Y386A, Y391A, coordinates relative to 950 residue isoform

AGO1 
piwi domain
necessary binding region
aa 691-950

coordinates relative to 950 residue isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0215456-8.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Anti-AGO1 immunoprecipitates mature ban miRNA almost exclusively, and not ban pre-miRNA.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0215487-3.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

AGO1 
AGO1 
AGO1 
AGO1 
AGO1 
AGO1 

Bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
interaction domain residues 594,629
mutation disrupting interaction
aa 594,629

F594V,F629V, coordinates relative to AGO1-PA

AGO1 
PAZ domain residues 386-388,446-448
mutation disrupting interaction
aa 386-388,446-448

R386A,K387A,Y388A,T446A,Y447A,L448A, coordinates relative to AGO1-PA

AGO1 
interaction domain residues 716,777
mutation disrupting interaction
aa 716,777

I716A,F777A, coordinates relative to AGO1-PA

AGO1 
interaction domain residues 785
mutation decreasing interaction
aa 785

K785A, coordinates relative to AGO1-PA

AGO1 
interaction domain residues 707
mutation decreasing interaction
aa 707

R707A, coordinates relative to AGO1-PA

AGO1 
interaction domain residues 749
mutation decreasing interaction
aa 749

Y749A, coordinates relative to AGO1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0220921-3.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In wild-type cells, AGO1 binds miRNA, and AGO2 binds siRNA. Upon r2d2 knockdown, AGO1 inappropriately binds siRNA.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0221629-1.FB.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

DmAgo1

bait

MBP tag

AGO1 

bantam

prey

[32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
C-terminal domain
sufficient binding region
aa 538-950

coordinates relative to AGO1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized and labeled in vitro.

FBrf0221629-3.FP.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

DmAgo1

bait

MBP tag

AGO1 

bantam

prey

[32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
N-terminal domain
mutation decreasing interaction
aa 1-538

coordinates relative to AGO1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized and labeled in vitro.

Deletion of the AGO1 N-terminus decreases its protection of the ban 3\' end.

FBrf0221629-4.coIP.NB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

DmAgo1

bait

FLAG tag

AGO1 
AGO1 

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
N-terminal domain
necessary binding region
aa 1-538

coordinates relative to AGO1-PB

AGO1 
N-terminal domain
mutation disrupting interaction
aa 352,353,354,412,413,414

R352A,K353A,Y354A,T412A,Y413A,L414A, coordinates relative to AGO1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0221629-5.coIP.FP.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

DmAgo1

bait

FLAG tag

AGO1 

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
N-terminal domain
mutation disrupting interaction
aa 619,623

Y619L,K623E, coordinates relative to AGO1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

RNA immunoprecipitated with AGO1 was hybridized to a labeled ban RNA probe and digested with a single strand specific RNase revealing full length and partially degraded ban bound to AGO1. N-terminally deleted or mutated AGO1 bound only the shorter partially degraded ban and to a lesser extent than wildtype.

FBrf0222591-1.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0224072-4.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

FLAG tag

ban

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0227265-1.coIP.NB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ban miRNA is preferentially sorted to AGO1 over AGO2.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

External Crossreferences and Linkouts ( 0 )
References (14)