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FB2026_01
,
released March 12, 2026
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Bait expressed in transiently transfected cells.
Prey expressed in transiently transfected cells.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
K1693A and N1698K mutations in gig did not affect binding
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Tsc2 variations to nonphosphorylatable alanine (S924A and T1518A) or phosphomimicking residues (S924D and T1518E) had no effect on the interaction.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene
Presence of insulin resulted in a mobility shift of Tsc2 but absence of insulin did not affect interaction.
SAINT score = 1
Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.
SAINT score = 1
Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Observed in two of three conditions tested: 10 minutes and 30 minutes after insulin stimulation.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.