Huang, J., Zhou, W., Watson, A.M., Jan, Y.N., Hong, Y. (2008). Efficient ends-out gene targeting in Drosophila.  Genetics 180(1): 703--707.

FBrf0206486

Research paper

In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.

PMC2535722 (PMC) (EuropePMC)