The information in the Associated file accompanied stocks donated to the Bloomington Drosophila Stock Center by the Transgenic RNAi Project, Harvard Medical School. The stocks fall into three categories as described below. Category 1. Each line was generated using the pCFD6 plasmid and contains two gRNAs that both target the same gene. Category 2. Each line was generated using the pCFD6 plasmid and contains a single gRNA. Category 3. Each line was generated using the pCFD6 plasmid and contains two gRNAs for two genes (four gRNAs in total).