FlyBase Insertion Report: Dmel\P{PZ}Dcp-1[02132]

General Information

Symbol

Dmel\P{PZ}Dcp-1⁰²¹³²

Species

D. melanogaster

Name

FlyBase ID

FBti0005211

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02132

Affected gene(s)

Dcp-1, Ecol\lacZ, pita

Viability / fertility

Associated allele(s)

Dcp-1⁰²¹³², Ecol\lacZ^Dcp-1-02132, pita⁰²¹³²

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2R ( 59E3 )

Sequence Location

2R:23,551,652..23,551,652 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:23,551,652..23,551,652 [+]

(Song et al., 1997)

Orientation

Cytological location (computed by FlyBase)

59E3 (inferred by FlyBase from sequence location)

Cytological location (reported)

59E3-59E4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2R:19439187..19439187 .

Sequence Data

Flanking sequence

G00735

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Dcp-1

(Nandi et al., 2014, McCall, 2004, Spradling et al., 1999, McCall and Steller, 1998, Song et al., 1997)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, McCall and Steller, 1998, Vosshall, 1998.8.10)

pita

(McCall, 2004, Laundrie et al., 2003, McCall et al., 2003, Spradling et al., 1999, FlyBase, 1996-)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Dcp-1⁰²¹³²

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^Dcp-1-02132

Phenotypic Class Phenotype Manifest in Detailed Description

pita⁰²¹³²

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Dcp-1

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02132

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

11179

cn¹ P{PZ}Dcp-1⁰²¹³² pita⁰²¹³²/CyO; ry⁵⁰⁶

Kyoto

108865

cn¹ P{PZ}Dcp-1⁰²¹³²/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

insertion position based on author statement that insertion maps 70bp upstream of the initiation codon and 291 bases downstream of the start of the cDNA.

(Song et al., 1997)

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P1179

(Meister and Braun, 1995.10)

P{PZ}Dcp-1⁰²¹³²

(Song et al., 1997, FlyBase, 1992-)

P{PZ}l(2)01862⁰²¹³²

(FlyBase, 1992-)

P{PZ}l(2)02132⁰²¹³²

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)01862⁰²¹³²

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02132⁰²¹³²

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000290
FBti0003025

References (18)

Report Sections

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