FlyBase Insertion Report: Dmel\P{GawB}NP4288

General Information

Symbol

Dmel\P{GawB}NP4288

Species

D. melanogaster

Name

FlyBase ID

FBti0035902

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP4288

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 44D2 )

Sequence Location

2R:8,535,064..8,535,718

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:8,535,064..8,535,718

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

44D2 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG215788

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Mitri et al., 2009)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP4288

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

gustatory receptor neuron of the labellum

(Mitri et al., 2009)

high salt/bitter-sensing neuron

(Mitri et al., 2009)

prothoracic leg taste bristle chemosensory neuron

(Mitri et al., 2009)

mesothoracic leg taste bristle chemosensory neuron

(Mitri et al., 2009)

metathoracic leg taste bristle chemosensory neuron

(Mitri et al., 2009)

labral sense organ neuron

(Mitri et al., 2009)

ventral cibarial sense organ neuron

(Mitri et al., 2009)

Additional Information

Statement

Reference

Reporter GFP expression is observed in taste organs. In the labellum, chemosensory sensilla contained one GFP-positive neuron. About 28 GFP-positive neurons are observed per labial palp and two in each tarsus of the legs. Four GFP-positive neurons are present in labral sense organs and in the ventral cibarial sense organs. Coexpression with ScerGAL4^Gr66a.PD suggests that it is expressed in bitter-sensistive gustatory receptor neurons.

(Mitri et al., 2009)

Marker for

Reflects expression of

mtt

(Mitri et al., 2009)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Mitri et al., 2009)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome