FlyBase Insertion Report: Dmel\Mi{Trojan-GAL4.2}VGlut1[MI04979-TG4.2]

General Information

Symbol

Dmel\Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}

Species

D. melanogaster

Name

FlyBase ID

FBti0168144

Feature type

transposable_element_insertion_site

Also Known As

vGlut-GAL4, VGlut^MI04979-Gal4

Description

Inserted Element

Mi{Trojan-GAL4.2}

LINE ID

Affected gene(s)

VGlut1

Viability / fertility

Associated allele(s)

Scer\GAL4^{VGlut1-MI04979-TG4.2}, VGlut1^{MI04979-TG4.2}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 22E1 )

Sequence Location

Target / Docking site

Mi{MIC}VGlut1^MI04979

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

22E1 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

Mi{Trojan-GAL4.2}

(Diao et al., 2015)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

VGlut1

(Keleş et al., 2020, Diao et al., 2015)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^{VGlut1-MI04979-TG4.2}

Phenotypic Class Phenotype Manifest in Detailed Description

VGlut1^{MI04979-TG4.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

glutamatergic neuron

(Diao et al., 2015)

embryonic/larval motor neuron

(Diao et al., 2015)

larval DA1 motor neuron

(Diao et al., 2015)

larval RP2 motor neuron

(Diao et al., 2015)

adult stage

DN1 neuron | subset

(Guo et al., 2016)

glutamatergic neuron

(Hill et al., 2019, Lacin et al., 2019)

medulla intrinsic neuron Mi9

(Davis et al., 2020)

adult glutamatergic neuron

(McKinney et al., 2020)

adult octopaminergic neuron | subset

(McKinney et al., 2020)

Additional Information

Statement

Reference

ScerGAL4^{VGlut-MI04979-TG4.2} drives expression in neurons in the adult thoracico-abdominal ganglion that express VGlut1 but not ChAT or GABA.

(Lacin et al., 2019)

Drives expression in glutamatergic neurons.

(FlyBase Curators, 2017)

ScerGAL4^{VGlut-MI04979-TG4.2} drives expression in adult neurons that use glutamate as a neurotransmitter, including a subset of DN1 neurons in the adult dorsal brain that coexpress EcollexA^GMR18H11.

(Guo et al., 2016)

ScerGAL4^{VGlut-MI04979-TG4.2} drives expression in neurons that use glutamate as a neurotransmitter, and replicates the VGlut1 immunoreactivity pattern. Expression is also observed in a small group of neurons in the posterior larval optic lobe; those neurons are not positive for VGlut1 immunoreactivity, but are also labeled by the VGlut1 reporter ScerGAL4^VGlut-OK371

(Diao et al., 2015)

Marker for

adult glutamatergic neuron

(McKinney et al., 2020)

Reflects expression of

VGlut1

(McKinney et al., 2020, Hill et al., 2019, Lacin et al., 2019, Bloomington Drosophila Stock Center, 2017, Guo et al., 2016, Diao et al., 2015)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Replacement descendant of (target)

Mi{MIC}VGlut1^MI04979

(Diao et al., 2015)

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

60312

w^*; Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}/CyO

External Crossreferences and Linkouts ( 0 )

Comments

phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 2 "Trojan GAL4" cassette from the pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70 plasmid.

(Diao et al., 2015)

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}

Mi{Trojan-GAL4.2}VGlut^{MI04979-TG4.2}

(Keleş et al., 2020)

VGluT-GAL4

(Yu et al., 2025, Chen et al., 2023)

VGlut-GAL4

(Silva et al., 2025, Bajar et al., 2022, Hill et al., 2019, Lacin et al., 2019, Riabinina et al., 2019, Schlichting et al., 2019)

VGlut^MI04979-GAL4

(Guo et al., 2016)

VGlut^MI04979-Gal4

(Diao et al., 2015)

Vglut-GAL4

(Ding et al., 2025)

Vglut^MI04979-TG4

(Bajar et al., 2022)

dVGlut-Gal4

(Sherer et al., 2020)

dvGlut-GAL4 ^MI04979

(Estacio-Gómez et al., 2020)

vGluT-Gal4

(King et al., 2020)

vGluT^MI04979-GAL4

(Ho et al., 2022)

vGluT^MI-GAL4

(Ho et al., 2022)

vGlut-GAL4

(Chaverra et al., 2026, Watanabe et al., 2017)

vGlut-Gal4

(McKinney et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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