FB2026_02 , released June 18, 2026
Recombinant Construct: M{gcm-FLAG.BAC}
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General Information
Symbol
M{gcm-FLAG.BAC}
FlyBase ID
FBtp0085751
Feature type
transgenic_transposable_element
Component Allele(s)
gcmTag:FLAG
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele gcmTag:FLAG
Transgenic product class
genomic_DNA
(Laneve et al., 2013)
wild_type
(Laneve et al., 2013)
Component
Use(s)
Regulatory region(s)
gcm
Encoded product / tool
gcm
Tagged with
Tag:FLAG
Description

Two copies of the Tag:FLAG tag has been inserted at the C-terminal end of the gcm coding region in the genomic fragment included in M{gcm.BAC}.

(Laneve et al., 2013)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5 -- 9
head mesoderm
(Laneve et al., 2013)
embryonic stage 9
embryonic longitudinal glial cell
(Laneve et al., 2013)
embryonic stage 10 -- 14
embryonic hemocyte primordium
(Laneve et al., 2013)
embryonic stage 11 -- 13
glial cell | subset of larval ventral nerve cord
(Laneve et al., 2013)
embryonic stage 11 -- 14
larval ventral nerve cord
(Laneve et al., 2013)
embryonic stage 12
tendon cell
(Laneve et al., 2013)
embryonic stage 13
embryonic/larval peritracheal cell
(Laneve et al., 2013)
embryonic stage 13 -- 16
glial cell | subset of larval brain
(Laneve et al., 2013)
neuron | subset of larval brain
(Laneve et al., 2013)
larval brain | lateral | restricted
(Laneve et al., 2013)
Additional Information
Statement
Reference

In the embryo, gcmT:ZzzzFLAG transiently labels a subset of glial cells in the ventral nerve cord, hemocyte precursors, tendon cells, two symmetric clusters in the procephalic mesoderm, interface glial cells and peritracheal cells in the abdomen, thorax and head. Expression in the ventral nerve cord glial cells starts at stage 10, earlier than expression of repo, with colocalisation observed in stages 11 to 13. Expression is also observed in a few elav-positive cells in the embryonic brain at stage 16. After stage 16, expression of gcmT:ZzzzFLAG disappears.

(Laneve et al., 2013)
 
Marker for
Reflects expression of
gcm
(Laneve et al., 2013)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
M{gcm.BAC}
(Laneve et al., 2013)
descendant(s)
Comments

Two copies of the T:Zzzz\FLAG tag has been inserted at the C-terminal end of the gcm coding region in the genomic fragment included in M{gcm.BAC}.

(Laneve et al., 2013)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
M{gcm-FLAG.BAC}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)