Allele Dmel\bi^omb-3198

General Information
Symbol	Dmel\bi^omb-3198	Species	D. melanogaster
Name		FlyBase ID	FBal0001123
Feature type	allele	Associated gene	Dmel\bi
Also Known As	omb³¹⁹⁸, l(1)omb³¹⁹⁸

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Kopp and Duncan, 1997, Tomlinson, 2003)
Mutagen	ethyl methanesulfonate (Pflugfelder et al., 1990, Poeck et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:4,344,915..4,344,916 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). evidence=experimental pr_change=W395@\|bi-PA reported_na_change=G?A reported_pr_change=W395@ (Poeck et al., 1993)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name S61742 S61743 AAB26699 S61744 AAB26699 S61955 AAB26699 S75261

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Point mutation causing premature termination of the protein. (Tsai et al., 1997) Nucleotide substitution: G?A. Amino acid replacement: W395. Lethal mutation is located within exon 4 around position -46, 336bp from the lethal mutation of bi^D4@. (Poeck et al., 1993) Point mutation. (Pflugfelder et al., 1992, Balles and Pflugfelder, 1994)
Cytology
Phenotypic Data
Phenotypic Class
	body color defective \| cell autonomous \| somatic clone (Lawrence et al., 2002) lethal (Pflugfelder et al., 1990) lethal \| pharate adult stage (del Alamo Rodriguez et al., 2004) lethal \| pupal stage \| recessive (Poeck et al., 1993, Pflugfelder et al., 1992) lethal \| recessive (Kopp and Duncan, 1997, Balles and Pflugfelder, 1994) planar polarity defective \| cell non-autonomous \| somatic clone (Lawrence et al., 2002) planar polarity defective \| somatic clone (Lawrence et al., 2002)
Phenotype Manifest In
	abdominal tergite \| posterior & trichome (Kopp and Duncan, 1997) adult cuticle & abdomen \| somatic clone \| cell autonomous (Lawrence et al., 2002) microchaeta \| somatic clone (Lawrence et al., 2002) tergite \| posterior (Kopp and Duncan, 1997) wing (del Alamo Rodriguez et al., 2004) wing disc (del Alamo Rodriguez et al., 2004) wing disc \| anterior/posterior compartment boundary \| somatic clone (Shen and Dahmann, 2005)
Detailed Description
	Statement Reference bi^omb-3198 clones generated along the A/P boundary of the wing imaginal disc, using the FLP/FRT technique, show a defective segregation phenotype. bi^omb-3198 clones of A, but not P, origin are partly misplaced into the territory of the adjacent compartment, causing a displacement of the A/P compartment boundary toward P. (Shen and Dahmann, 2005) Mutants die as pharate adults. A deletion of the central wing disc is seen, also huge overgrowths are seen that do not evaginate, but remain internal. (del Alamo Rodriguez et al., 2004) Clones of bi^omb-3198 that are induced in the dorsal half of the eye do not remove the dorsal rim ommatidia. (Tomlinson, 2003) When somatic clones are made in the segmental anterior 4 (a4) region of the anterior segmental compartment in the adult abdomen an associated patch of reversed polarity is seen, which can extend in front of and behind the clone. The clone itself also lacks the dark a4 pigment. When the clone is near the back of the anterior compartment hairs (and thus polarity) are reversed. This reversed polarity effect only extends as far as the anterior posterior boundary. When somatic clones are made in the segmental posterior 3 (p3) region of the posterior segmental compartment in the adult abdomen, about one third of the clones lost some, but not all of the hairs within the clone. (Lawrence et al., 2002) Hemizygotes usually die at the late larval or early pupal stage, with a few animals surviving to the late pharate adult stage. Homozygous clones in the abdomen have no effect on polarity or cell fates when located in the anterior tergite. Clones in the posterior tergite and posterior hairy zone cause reversal of trichome polarity and disappearance of the pigment band. Clones in the posterior tergite are small, even if induced at early embryonic stages. (Kopp and Duncan, 1997) Neuroanatomical omb phenotype. (Poeck et al., 1993) Displays lac and rb phenotypes. (Pflugfelder et al., 1990)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement Reference bi^omb-3198 is a non-suppressor of increased cell number \| somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.Scer\UAS.cNc} (Martin-Castellanos and Edgar, 2002)
Phenotype Manifest In
Suppressed by
Statement Reference bi^omb-3198 has wing disc \| anterior/posterior compartment boundary \| somatic clone phenotype, suppressible by ci^Scer\UAS.cDa/Scer\GAL4^hh-Gal4 (Shen and Dahmann, 2005) bi^omb-3198 has wing disc phenotype, suppressible \| partially by Scer\GAL4^nub-AC-62/puc^Scer\UAS.cMa (del Alamo Rodriguez et al., 2004)
NOT suppressed by
Statement Reference bi^omb-3198 has wing disc phenotype, non-suppressible \| partially by Scer\GAL4^nub-AC-62/puc^Scer\UAS.cMa (del Alamo Rodriguez et al., 2004)
Suppressor of
Statement Reference bi^omb-3198 is a suppressor of wing disc \| anterior compartment \| somatic clone phenotype of Scer\GAL4^hh-Gal4, ci^Scer\UAS.cDa (Shen and Dahmann, 2005)
Additional Comments
Genetic Interactions
Statement Reference Clones generated along the A/P boundary of the wing imaginal disc, that overexpress ci^Scer\UAS.cDa, driven by Scer\GAL4^hh-Gal4, in a bi^omb-3198 background show no mis-segregation phenotype. (Shen and Dahmann, 2005) When bi^omb-3198 ptc¹⁶ double homozygous clones are made in the anterior compartment of the abdominal segment. These clones makes segmental region anterior 6 (a6) cuticle, like ptc¹⁶ clones, but reverse polarity in the front half of the clone as do bi^omb-3198 clones. (Lawrence et al., 2002) When tkv^{Q253D.Scer\UAS.cNc} is driven by Scer\GAL4^Act5C.PP in a bi^omb-3198 background, no suppression of the proliferation phenotype is seen. (Martin-Castellanos and Edgar, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	bi^omb-3198 Df(1)omb3198 l(1)bi² l(1)omb3198 (Shen et al., 2008) l(1)omb³¹⁹⁸ (Kopp and Duncan, 1997, Tsai et al., 1997, Grimm and Pflugfelder, 1996, Balles and Pflugfelder, 1994, Pflugfelder et al., 1990) omb³¹⁹⁸ (Shen and Dahmann, 2005, Tomlinson, 2003, del Alamo Rodriguez et al., 2004, Takatsu et al., 2000, Poeck et al., 1993, Pflugfelder et al., 1992) omb^- (Lawrence et al., 2002)
Name Synonym
Secondary FlyBase IDs
	FBab0000866
References ( 14 )
Research paper	Shen et al., 2008, Mech. Dev. 125(3-4): 233--246 optomotor-blind suppresses instability at the A/P compartment boundary of the Drosophila wing. [FBrf0202782] Shen and Dahmann, 2005, Dev. Biol. 279(1): 31--43 The role of Dpp signaling in maintaining the Drosophila anteroposterior compartment boundary. [FBrf0183859] del Alamo Rodriguez et al., 2004, Dev. Biol. 268(2): 481--492 The role of the T-box gene optomotor-blind in patterning the Drosophila wing. [FBrf0174525] Tomlinson, 2003, Dev. Cell 5(5): 799--809 Patterning the peripheral retina of the fly: decoding a gradient. [FBrf0167480] Lawrence et al., 2002, Development 129(11): 2749--2760 Towards a model of the organisation of planar polarity and pattern in the Drosophila abdomen. [FBrf0148964] Martin-Castellanos and Edgar, 2002, Development 129(4): 1003--1013 A characterization of the effects of Dpp signaling on cell growth and proliferation in the Drosophila wing. [FBrf0144813] Takatsu et al., 2000, Mol. Cell. Biol. 20(9): 3015--3026 TAK1 participates in c-Jun N-terminal kinase signaling during Drosophila development. [FBrf0127351] Kopp and Duncan, 1997, Development 124(19): 3715--3726 Control of cell fate and polarity in the adult abdominal segments of Drosophila by optomotor-blind. [FBrf0099019] Tsai et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94(8): 3837--3841 Gypsy retrotransposon as a tool for the in vivo analysis of the regulatory region of the optomotor-blind gene in Drosophila. [FBrf0093233] Grimm and Pflugfelder, 1996, Science 271(5255): 1601--1604 Control of the gene optomotor-blind in Drosophila wing development by decapentaplegic and wingless. [FBrf0086426] Balles and Pflugfelder, 1994, Mol. Gen. Genet. 245(6): 734--740 Facilitated isolation of rare recombinants by ligase chain reaction: Selection for intragenic crossover events in the Drosophila optomotor-blind gene. [FBrf0078013] Poeck et al., 1993, Mol. Gen. Genet. 238(3): 325--332 Transcript identification in the optomotor-blind locus of Drosophila melanogaster by intragenic recombination mapping and PCR-aided sequence analysis of lethal point mutations. [FBrf0059076] Pflugfelder et al., 1992, Proc. Natl. Acad. Sci. U.S.A. 89: 1199--1203 The lethal(1)optomotor-blind gene of Drosophila melanogaster is a major organizer of optic lobe development: isolation and characterization of the gene. [FBrf0057510] Pflugfelder et al., 1990, Genetics 126: 91--104 Genetic and molecular characterization of the optomotor-blind gene locus in Drosophila melanogaster. [FBrf0051990]

Allele Dmel\biomb-3198

Allele Dmel\bi^omb-3198