Allele Dmel\wun^Scer\UAS.cZa

General Information
Symbol	Dmel\wun^Scer\UAS.cZa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Zhang	FlyBase ID	FBal0055781
Feature type	allele	Associated gene	Dmel\wun

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Zhang et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of wun cDNA c18 is governed by Scer\UAS regulatory sequences. (Zhang et al., 1997)
Carried in construct	P{UAS-wun.Z} (Renault et al., 2004, Starz-Gaiano et al., 2001, Zhang et al., 1997, Renault et al., 2004, Renault et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	germline cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Starz-Gaiano et al., 2001) germline cell \| female, with Scer\GAL4^twi.PG (Zhang et al., 1997) germline cell \| male, with Scer\GAL4^twi.PG (Zhang et al., 1997)
Detailed Description
	Statement Reference Expression of wun^Scer\UAS.cZa under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B results in a reduction in germ cell number. (Starz-Gaiano et al., 2001) When expression is governed by Scer\GAL4^twi.PG, such that wun is expressed in the mesoderm, very few germ cells enter the mesoderm, remaining instead in the yolk. (Zhang et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor of decreased cell number \| germline clone \| maternal effect \| embryonic stage 15 phenotype of wun2^EP2650ex34, wun⁴⁹ (Renault et al., 2010) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor of decreased cell number \| germline clone \| maternal effect \| embryonic stage 16 phenotype of wun2^EP2650ex34, wun⁴⁹ (Renault et al., 2010) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor of increased cell death \| germline clone \| maternal effect \| embryonic stage phenotype of wun2^EP2650ex34, wun⁴⁹ (Renault et al., 2010)
Phenotype Manifest In
Enhancer of
Statement Reference wun^Scer\UAS.cZa/Scer\GAL4^ninaE.PT is an enhancer of eye photoreceptor cell phenotype of rdgA³ (Garcia-Murillas et al., 2006)
Suppressor of
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor \| partially of germline cell phenotype of wun2^{αTub84B.T:Hsap\MYC} (Renault et al., 2004) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor of germline cell \| germline clone \| maternal effect \| embryonic stage 15 phenotype of wun2^EP2650ex34, wun⁴⁹ (Renault et al., 2010) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor of germline cell \| germline clone \| maternal effect \| embryonic stage 16 phenotype of wun2^EP2650ex34, wun⁴⁹ (Renault et al., 2010) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/wun^Scer\UAS.cZa is a suppressor of germline cell \| maternal effect phenotype of wun2^EP2650ex34/Df(2R)wun-GL (Renault et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Expression of wun[Scer\UAS.cZa] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] rescues the germ cell death in embryos derived from females carrying homozygous wun[49] wun2[EP2650ex34] germline clones and wild type males. (Renault et al., 2010) The rate of retinal degeneration seen in rdgA³ flies is enhanced if they are also expressing wun^Scer\UAS.cZa under the control of Scer\GAL4^ninaE.PT. (Garcia-Murillas et al., 2006) The germ cell death seen in embryos with a wun2⁺ soma which are derived from wun2^EP2650ex34/Df(2R)wun-GL females is rescued by expression of wun^Scer\UAS.cZa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. Expression of wun^Scer\UAS.cZa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2^{αTub84B.T:Hsap\MYC} in the soma. (Renault et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	wun^Scer\UAS.cZa wun^UAS.cZa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Zhang (Zhang et al., 1997)
Secondary FlyBase IDs

References ( 6 )
Research paper	Renault et al., 2010, Development 137(11): 1815--1823 Lipid phosphate phosphatase activity regulates dispersal and bilateral sorting of embryonic germ cells in Drosophila. [FBrf0210764] Renault et al., 2004, Science 305(5692): 1963--1966 Soma-germ line competition for lipid phosphate uptake regulates germ cell migration and survival. [FBrf0180678] Starz-Gaiano et al., 2001, Development 128(6): 983--991 Spatially restricted activity of a Drosophila lipid phosphatase guides migrating germ cells. [FBrf0134584] Zhang et al., 1997, Nature 385(6611): 64--67 The Drosophila protein Wunen repels migrating germ cells. [FBrf0091204]
Supplementary material	Garcia-Murillas et al., 2006, Neuron 49(4): Supplementary data. [FBrf0202553] Renault et al., 2004, Science 305(5692): Supporting Online Material. [FBrf0202565]

Allele Dmel\wunScer\UAS.cZa

Allele Dmel\wun^Scer\UAS.cZa