Allele Dmel\14-3-3ζ^P1188

General Information
Symbol	Dmel\14-3-3ζ^P1188	Species	D. melanogaster
Name		FlyBase ID	FBal0059629
Feature type	allele	Associated gene	Dmel\14-3-3ζ
Also Known As	leo^P1188

Allele class	hypomorphic allele - genetic evidence
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Broadie et al., 1997)
Mutagen	Delta2-3 (Han et al., 1996, Skoulakis and Davis, 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lArB}A4.1M2 (Han et al., 1996, Skoulakis and Davis, 1996)
Nature of the lesion	Statement Reference Insertion, in forward orientation, of P{lArB} into the intron between exons 1' and 2 of 14-3-3ζ. (Philip et al., 2001) P{lArB} insertion in the first intron. (Broadie et al., 1997)
Caused by insertion	P{lArB}14-3-3ζ^P1188 (Han et al., 1996, Su et al., 2001, Philip et al., 2001, Benton et al., 2002, Broadie et al., 1997, Skoulakis and Davis, 1996)
Cytology
Phenotypic Data
Phenotypic Class
	learning defective (with 14-3-3ζ^P1375), with 14-3-3ζ^LI.15.hs (Philip et al., 2001) learning defective (with 14-3-3ζ^P1375), with 14-3-3ζ^LI.15.hs, 14-3-3ζ^LII.2.hs (Philip et al., 2001) learning defective (with 14-3-3ζ^P2335), with 14-3-3ζ^LI.15.hs (Philip et al., 2001) learning defective (with 14-3-3ζ^P2335), with 14-3-3ζ^LI.15.hs, 14-3-3ζ^LII.2.hs (Philip et al., 2001) lethal (Philip et al., 2001, Benton et al., 2002, Messaritou et al., 2009) lethal (with 14-3-3ζ^P2335) (Messaritou et al., 2010, Messaritou et al., 2009) lethal \| embryonic stage \| recessive (Skoulakis and Davis, 1996) lethal \| recessive (Broadie et al., 1997, Messaritou et al., 2010) mitotic cell cycle defective \| maternal effect \| recessive (Su et al., 2001) neurophysiology defective (Broadie et al., 1997) viable (with 14-3-3ζ^P1375), with 14-3-3ζ^LI.15.hs (Philip et al., 2001) viable (with 14-3-3ζ^P1375), with 14-3-3ζ^LI.15.hs, 14-3-3ζ^LII.2.hs (Philip et al., 2001) viable (with 14-3-3ζ^P2335), with 14-3-3ζ^LI.15.hs (Philip et al., 2001) viable (with 14-3-3ζ^P2335), with 14-3-3ζ^LI.15.hs, 14-3-3ζ^LII.2.hs (Philip et al., 2001)
Phenotype Manifest In
	border follicle cell \| somatic clone (McDonald et al., 2008) denticle belt \| germline clone (Li et al., 1997) embryonic/first instar larval cuticle \| germline clone (Li et al., 1997) follicle cell \| somatic clone (Benton and St. Johnston, 2003) mitotic cell cycle \| maternal effect (Su et al., 2001) syncytial blastoderm embryo \| germline clone (Li et al., 1997)
Detailed Description
	Statement Reference 12-18% of 14-3-3ζ[P1188]/14-3-3ζ[P2335] heteroallelic animals are escapers. They do not exhibit external defects or grossly aberrant behaviors, despite no 14-3-3ζ expression. (Messaritou et al., 2009) Border follicle cell migration is disrupted in mosaic egg chambers containing homozygous follicle cell clones. (McDonald et al., 2008) Large homozygous follicle cell clones show dramatic defects in tissue organisation, forming multilayers of morphologically abnormal cells that often fail to encapsulate germline cysts properly. Smaller homozygous follicle cell clones that arise after epithelium formation have milder and less penetrant defects in morphology and polarity. (Benton and St. Johnston, 2003) 14-3-3ζ^P1188 germ-line clones do not exhibit defects in oocyte specification or polarization. (Benton et al., 2002) Flies rescued from lethality by 14-3-3ζ^LI.15.hs or 14-3-3ζ^LI.15.hs and 14-3-3ζ^LII.2.hs show a 25-30% decrement in olfactory learning, comparable to that of (rescued) 14-3-3ζ^2.3. The restoration of learning by the transgenes decays back to mutant levels 60-70 hr later. (Philip et al., 2001) Homozygous embryos show no apparent defects in the timing of mitotic cycle 14 and show delayed mitosis after irradiation (as occurs in wild type). 69 +/- 9% of mutant embryos derived from homozygous female germline clones fail to cellularise. 54/59 of the embryos have defects in cell division, including DNA bridges between telophase sister nuclei, asynchrony in division within a single embryo, free microtubule-organizing centres that are not associated with nuclei, loss of nuclei from the cortical monolayer of nuclei and larger than normal yolk DNA masses. Chromosome bridges interconnecting DNA masses are seen as early as telophase of the fourth embryonic mitosis. Mitotic spindles do appear to be formed in these embryos (as judged by the segregation of chromosome masses that are still linked by DNA bridges to opposite spindle poles), and attempts at the formation of mid-bodies are seen between segregating nuclei, despite the presence of chromosome bridges. Approximately 30% of embryos cellularise. These embryos have severe gastrulation defects. (Su et al., 2001) Embryos do not exhibit a dorsal closure defect. The amplitude and frequency of endogenous excitatory junctional currents (EJCs) is reduced relative to wild type and the NMJ exhibits a transmission defect, the calcium dependence curve is shifted to the right indicating a higher level of external calcium is required to achieve the given level of secretion. (Broadie et al., 1997) Embryos derived from homozygous female germline clones show a variable phenotype that is not significantly different whether or not the embryos contain a wild-type copy of 14-3-3ζ from the father. Approximately 50% of the embryos do not develop cuticles, and the remainder develop cuticles with various segmentation defects including missing and/or fused denticle bands. The Filzkorper appear normal. Approximately 50% of the embryos appear to stop development during the syncytial blastoderm stage, and contain many fewer nuclei compared to wild-type. Some of these nuclei appear fused. 18% of embryos carrying 14-3-3ζ^hbNRE.RpII15 and derived from homozygous 14-3-3ζ^P1188 female germline clones have a wild-type anterior region but are missing all or part of the posterior region. 56% of these 'anteriorly rescued' embryos have shortened Filzkorper. (Li et al., 1997) Heterozygotes exhibit normal 3 minute memory performance. Odour avoidance (octanol and benzaldehyde) is normal. (Skoulakis and Davis, 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference 14-3-3ζ^P1188 is an enhancer of nurse cell \| ectopic \| germline clone phenotype of 14-3-3ε^j2B10 (Benton et al., 2002) 14-3-3ζ^P1188 is an enhancer of oocyte \| germline clone phenotype of 14-3-3ε^j2B10 (Benton et al., 2002)
Suppressor of
Statement Reference 14-3-3ζ^P1188 is a suppressor \| maternal effect of embryonic/first instar larval cuticle \| maternal effect phenotype of tor^12D (Li and Li, 2003)
Other
Statement Reference 14-3-3epsilon[+]/14-3-3ε^S-1259, 14-3-3ζ^P1188 has oocyte \| germline clone phenotype (Benton et al., 2002) 14-3-3ε^j2B10, 14-3-3ζ^P1188 has oocyte \| germline clone phenotype (Benton et al., 2002) 14-3-3ε^j2B10, 14-3-3ζ^P1375/14-3-3ζ^P1188 has follicle cell phenotype (Benton and St. Johnston, 2003) 14-3-3ε^j2B10/14-3-3epsilon[+], 14-3-3ζ^P1188 has oocyte \| germline clone phenotype (Benton et al., 2002) 14-3-3ε^j2B10/14-3-3epsilon[+], 14-3-3ζ^P1375/14-3-3ζ^P1188 has follicle cell phenotype (Benton and St. Johnston, 2003) 14-3-3ε^S-1259, 14-3-3ζ^P1188 has oocyte \| germline clone phenotype (Benton et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Follicular epithelium morphogenesis is normal in 14-3-3ζ^P1188/14-3-3ζ^P1375 ; 14-3-3ε^j2B10/+ egg chambers up to stage 4, but the follicle cells subsequently lose their regular cuboidal shape. (Benton and St. Johnston, 2003) Many 14-3-3ζ^P1188 germ-line clones in 14-3-3ε^j2B10/+ females have defects in oocyte specification and polarization. Many 14-3-3ζ^P1188 germ-line clones in 14-3-3ε^S-1259/+ females have defects in oocyte specification. The penetrance of the oocyte to nurse cell transformation phenotype seen in 14-3-3ε^j2B10 germ-line clones (80% n=106) is dominantly enhanced to 100% by 14-3-3ζ^P1188. (Benton et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	14-3-3ζ^P1188 is rescued by 14-3-3ζ^I.Scer\UAS/Scer\GAL4^elav.PLu (Messaritou et al., 2009) 14-3-3ζ^P1188 is rescued by 14-3-3ζ^II.Scer\UAS/Scer\GAL4^elav.PLu (Messaritou et al., 2009) 14-3-3ζ^P1188 is rescued by 14-3-3ζ^{III.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^elav.PLu (Messaritou et al., 2009) 14-3-3ζ^P1375/14-3-3ζ^P1188 is rescued by 14-3-3ζ^LI.15.hs (Philip et al., 2001) 14-3-3ζ^P2335/14-3-3ζ^P1188 is rescued by 14-3-3ζ^I.Scer\UAS/Scer\GAL4^elav.PLu (Messaritou et al., 2009) 14-3-3ζ^P2335/14-3-3ζ^P1188 is rescued by 14-3-3ζ^II.Scer\UAS/Scer\GAL4^elav.PLu (Messaritou et al., 2009) 14-3-3ζ^P2335/14-3-3ζ^P1188 is rescued by 14-3-3ζ^{III.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^elav.PLu (Messaritou et al., 2009) 14-3-3ζ^P2335/14-3-3ζ^P1188 is rescued by 14-3-3ζ^LI.15.hs (Philip et al., 2001)
Partially rescued by	14-3-3ζ^P1188 is partially rescued by Scer\GAL4^elav.PU/14-3-3ζ^{MW.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010, Messaritou et al., 2010) 14-3-3ζ^P1188 is partially rescued by Scer\GAL4^elav.PU/14-3-3ζ^{WW.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010, Messaritou et al., 2010) 14-3-3ζ^P2335/14-3-3ζ^P1188 is partially rescued by Scer\GAL4^elav.PU/14-3-3ζ^{MW.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010) 14-3-3ζ^P2335/14-3-3ζ^P1188 is partially rescued by Scer\GAL4^elav.PU/14-3-3ζ^{WW.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010)
Not rescued by	14-3-3ζ^P1188 is not rescued by 14-3-3ζ^LI.15.hs (Philip et al., 2001) 14-3-3ζ^P1188 is not rescued by 14-3-3ζ^LII.2.hs (Philip et al., 2001) 14-3-3ζ^P1188 is not rescued by Scer\GAL4^elav.PU/14-3-3ζ^{MM.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010, Messaritou et al., 2010) 14-3-3ζ^P1188 is not rescued by Scer\GAL4^elav.PU/14-3-3ζ^{WM.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010, Messaritou et al., 2010) 14-3-3ζ^P2335/14-3-3ζ^P1188 is not rescued by Scer\GAL4^elav.PU/14-3-3ζ^{MM.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010) 14-3-3ζ^P2335/14-3-3ζ^P1188 is not rescued by Scer\GAL4^elav.PU/14-3-3ζ^{WM.Scer\UAS.T:Zzzz\FLAG} (Messaritou et al., 2010)
Comments	Expression of 14-3-3ζ[I.Scer\UAS] under the control of Scer\GAL4[elav.PLu] partially rescues the lethality found in 14-3-3ζ[P1188] homozygotes and 14-3-3ζ[P1188]/14-3-3ζ[P2335] to approximately 20% of expected. Expression of 14-3-3ζ[II.Scer\UAS] under the control of Scer\GAL4[elav.PLu] partially rescues the lethality found in 14-3-3ζ[P1188] homozygotes and 14-3-3ζ[P1188]/14-3-3ζ[P2335] heterozygotes to approximately 15-20% of expected. Expression of 14-3-3ζ[III.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav.PLu] partially rescues the lethality found in 14-3-3ζ[P1188] homozygotes and 14-3-3ζ[P1188]/14-3-3ζ[P2335] heterozygotes to approximately 60-70% of expected. (Messaritou et al., 2009)
Stocks ( 1 )
Bloomington	9402 P{lArB}14-3-3ζ^P1188/CyO; ry⁵⁰⁶
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	14-3-3^P1188 14-3-3ζ^P1188 leo^P1188 (Benton and St. Johnston, 2003, Li and Li, 2003, Benton et al., 2002, Philip et al., 2001, Li et al., 2000, Li et al., 1997, Acevedo et al., 2007, McDonald et al., 2008, Messaritou et al., 2010, Messaritou et al., 2009) P1188 (Su et al., 2001) unnamed (Skoulakis and Davis, 1996, Han et al., 1996)
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Messaritou et al., 2010, J. Biol. Chem. 285(3): 1692--1700 Dimerization is essential for 14-3-3zeta stability and function in vivo. [FBrf0209680] Messaritou et al., 2009, FEBS Lett. 583(17): 2934--2938 A third functional isoform enriched in mushroom body neurons is encoded by the Drosophila 14-3-3zeta gene. [FBrf0208682] McDonald et al., 2008, Curr. Biol. 18(21): 1659--1667 PAR-1 Kinase Regulates Epithelial Detachment and Directional Protrusion of Migrating Border Cells. [FBrf0206227] Acevedo et al., 2007, Genetics 177(1): 239--253 In vivo functional specificity and homeostasis of Drosophila 14-3-3 proteins. [FBrf0201747] Benton and St. Johnston, 2003, Cell 115(6): 691--704 Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells. [FBrf0173208] Li and Li, 2003, Genetics 164(1): 247--258 Drosophila gain-of-function mutant RTK Torso triggers ectopic Dpp and STAT signaling. [FBrf0158996] Benton et al., 2002, Dev. Cell 3(5): 659--671 Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation. [FBrf0151902] Philip et al., 2001, J. Neurosci. 21(21): 8417--8425 Conditional rescue of olfactory learning and memory defects in mutants of the 14-3-3 gene leonardo. [FBrf0139731] Su et al., 2001, J. Cell Sci. 114(19): 3445--3454 Cell cycle roles for two 14-3-3 proteins during Drosophila development. [FBrf0139721] Li et al., 2000, Genetics 156(2): 763--774 Identification of autosomal regions involved in Drosophila raf function. [FBrf0129944] Broadie et al., 1997, Neuron 19(2): 391--402 Leonardo, a Drosophila 14-3-3 protein involved in learning, regulates presynaptic function. [FBrf0098203] Li et al., 1997, Development 124(20): 4163--4171 The Drosophila 14-3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner. [FBrf0099365] Han et al., 1996, J. Neurobiol. 31(1): 88--102 The Drosophila brain revisited by enhancer detection. [FBrf0089671] Skoulakis and Davis, 1996, Neuron 17(5): 931--944 Olfactory learning deficits in mutants for leonardo, a Drosophila gene encoding a 14-3-3 protein. [FBrf0090801]

Allele Dmel\14-3-3ζP1188

Allele Dmel\14-3-3ζ^P1188