FlyBase Allele Report: Dmel\Sumo[k06307]

General Information

Symbol

Dmel\Sumo^k06307

Species

D. melanogaster

Name

FlyBase ID

FBal0064719

Feature type

allele

Associated gene

Dmel\Sumo

Associated Insertion(s)

P{lacW}Sumo^k06307

Carried in Construct

Also Known As

smt3^k06307, P{lacW}smt3^k06307

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Sumo^k06307

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(BDGP Project Members, 1994-1999)

Phenotype Manifest In

embryonic/larval crystal cell

(Milchanowski et al., 2004)

Detailed Description

Statement

Reference

Average Citrate Synthase activity is increased by 17% in heterozygous males

(Chen et al., 2008)

The average number of crystal cells per embryo is reduced (to 7.6) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).

(Milchanowski et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sumo^k06307/smt3[+] is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

Sumo^k06307/smt3[+] is a suppressor of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Sumo^k06307/smt3[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

lwr⁰²⁸⁵⁸/Sumo^k06307 is an enhancer of wing margin phenotype of ct⁶, mod(mdg4)^ul

(Capelson and Corces, 2006)

NOT Enhancer of

Statement

Reference

Sumo^k06307/smt3[+] is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, p53^UAS.Ex

(Pardi et al., 2011)

lwr⁰⁵⁴⁸⁶, Sumo^k06307, smt3[+], lwr[+] is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, p53^UAS.Ex

(Pardi et al., 2011)

Suppressor of

Statement

Reference

Sumo^k06307/smt3[+] is a suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

lwr⁰²⁸⁵⁸/Sumo^k06307 is a suppressor of adult cuticle phenotype of ct⁶, mod(mdg4)^ul

(Capelson and Corces, 2006)

lwr⁰²⁸⁵⁸/Sumo^k06307 is a suppressor of adult cuticle phenotype of mod(mdg4)^ul, y²

(Capelson and Corces, 2006)

lwr⁰²⁸⁵⁸/Sumo^k06307 is a suppressor of wing margin phenotype of ct⁶, mod(mdg4)^ul

(Capelson and Corces, 2006)

NOT Suppressor of

Statement

Reference

Sumo^k06307/smt3[+] is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, p53^UAS.Ex

(Pardi et al., 2011)

lwr⁰⁵⁴⁸⁶, Sumo^k06307, smt3[+], lwr[+] is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, p53^UAS.Ex

(Pardi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A loss of heterozygosity assay, using mwh¹ as a marker, reveals that a smt3^k06307 heterozygous background increases the number of mwh¹ clones after irradiation with 250 rad of X-rays, in p53^5A-1-4 mutants.

The rough eye phenotype found at 29[o]C in flies expressing p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PF is not affected by a smt3^k06307 heterozygous background nor in a lwr⁰⁵⁴⁸⁶-smt3^k06307 transheterozygous background.

(Pardi et al., 2011)

A smt3^k06307/+ background suppresses the visual system degeneration seen in Scer\GAL4^GMR.PF, Ppt1^Scer\UAS.cKa flies.

(Saja et al., 2010)

The transheterozygote lwr⁰²⁸⁵⁸/smt3^k06307 suppresses the mod(mdg4)^ul suppression of the ct⁶ jagged wing phenotype, leading to a more jagged and discontinuous wing phenotype. The phenotype is also particularly pronounced in females, although it is detectable in both sexes.

The transheterozygote lwr⁰²⁸⁵⁸/smt3^k06307 suppresses the mod(mdg4)^ul suppression of the y² cuticle phenotype, leading to a lighter coloration of the abdomen (due to a reduction in y expression). These phenotypic changes occur in approximately 30% of the mutant flies, with the remaining 70% displaying a continuum of less-pronounced effect. The phenotype is also particularly pronounced in females, although it is detectable in both sexes.

(Capelson and Corces, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Sumo⁰⁴⁴⁹³

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10419

y¹ w^67c23; P{lacW}Sumo^k06307/CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: Hrb27C⁰²⁶⁴⁷. Complements: Rca1⁰³³⁰⁰. Complements: xl6^k00230.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P10419

(Milchanowski et al., 2004)

Smt3^K06307

(Kalamarz et al., 2012)

Sumo^k06307

l(2)04493^k06307

(BDGP Project Members, 1994-1999)

smt3^K06307

(Chen et al., 2008)

smt3^k06307

(Saja et al., 2010, Simonsen et al., 2007, Capelson and Corces, 2006)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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