SUMO, dSmt3, DmSmt3, l(2)04493, Small Ubiquitin-like Modifier
a low molecular weight protein modifier that is vital for multicellular development - over 140 SUMO conjugates have been identified in the early embryo including Dorsal, Tramtrack, Vestigial, SoxNeuro, Medea, Mod(mdg4) and CP190 and the bi-functional tRNA charging enzyme glutamylprolyl-tRNA synthetase
Gene model reviewed during 5.49
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.44
Gene model reviewed during 6.02
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\smt3 using the Feature Mapper tool.
Maternal smt3 transcripts are distributed throughout the embryo until stage 10 and then decrease gradually. From stage 14 until the end of embryogenesis, transcripts are observed in the embryonic gonads in the pole cells. smt3 is also strongly expressed in the CNS. Within ovaries, transcripts are weakly detected in the germarium, are strongly expressed in stage S1-10 nurse cells and are weakly detected in the oocyte up until stage S10. smt3 transcripts are weakly expressed in spermatogonial cells at the tip region and remain detectable in the spermatocytes and spermatids.
GBrowse - Visual display of RNA-Seq signalsView Dmel\smt3 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: smt3 SUMO
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in the formation of monastral bipolar spindles when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
Expression is enriched in embryonic gonads.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against smt3 that is transfected into S2 treated with Listeria monocytogenes reveals smt3 to be involved in Listeria monocytogenes intracellular growth.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
Area matching Drosophila gene SMT3, Acc. No. AF053083.