|Name||Saccharomyces cerevisiae UAS construct a of Buescher||FlyBase ID||FBal0178887|
|Feature type||allele||Associated gene||Dmel\mid|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Scer\GAL4[bi-omb-Gal4]-driven expression of mid[Scer\UAS.cBa] results in ectopic sex combs and transverse bristle rows in the dorsal basitarsus and distal tibia of all male first legs. In the second leg, ectopic expression of Scer\GAL4[bi-omb-Gal4]-driven mid[Scer\UAS.cBa] in the dorsal tibia results in ectopic bristles similar to the apical and spur bristles. Scer\GAL4[Act5C.PI]-driven expression of mid[Scer\UAS.cBa] in small somatic clones also results in the appearance of similar ectopic bristles. Small clones of mid[Scer\UAS.cBa]-expressing cells either in or adjacent to rows 2/7 and 3/6 induce ventral row 1/8 bristles cell autonomously. Ventrolateral or dorsolateral Scer\GAL4[Act5C.PI]-driven mid[Scer\UAS.cBa]-expressing clones in the tarsus are often transformed to ventral character.
By stage 17, phenotypic effects of ectopic midScer\UAS.cBa expression in the mesoderm, driven by Scer\GAL4twi.PB, can be observed; dorsal vessel development is disrupted and in some embryos the two halves of the heart fail to fuse. The same phenotype is observed when midScer\UAS.cBa is expressed in the dorsal mesoderm and ectoderm under the control of Scer\GAL4pnr-MD237. Expression of midScer\UAS.cBa throughout the mesoderm, under the control of Scer\GAL4how-24B, causes the ectopic formation of up to 30 extra cardioblasts per half dorsal vessel, but only rarely disrupts the fusing of the two halves of the heart.
Expression of midScer\UAS.cBa under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4sca-P309 results in nearly all naked cuticle being replaced by denticles in the posterior half of the larva. Larvae expressing midScer\UAS.cBa under the control of Scer\GAL4ptc-559.1 have a reduced body size, strong segmental indentations and head malformations. Expression of midScer\UAS.cBa under the control of Scer\GAL4prd.RG1 results in larvae with fusion of alternating denticle belts.
|Phenotype Manifest In|
Mmus\Lef1Scer\UAS.cRa, Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, midScer\UAS.cBa has mesothoracic tarsal bristle phenotype
Mmus\Lef1Scer\UAS.cRa, Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, midScer\UAS.cBa has metathoracic tarsal bristle phenotype
Clones expressing both mid[Scer\UAS.cBa] and Mmus\Lef1[Scer\UAS.cRa] in the tarsus driven by Scer\GAL4[bi-omb-Gal4] are recovered more often in lateral and dorsal cells, indicating altered sorting behaviour. Dorsolateral clones expressing both mid[Scer\UAS.cBa] and Mmus\Lef1[Scer\UAS.cRa] do not transform towards ventral fate, whereas ventrolateral clones do so sometimes.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Buescher
|Secondary FlyBase IDs|
|References ( 6 )|