Allele Dmel\mid^Scer\UAS.cBa

General Information
Symbol	Dmel\mid^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Buescher	FlyBase ID	FBal0178887
Feature type	allele	Associated gene	Dmel\mid

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Buescher et al., 2004, Miskolczi-McCallum et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a full length mid cDNA (derived from RE27439). (Buescher et al., 2004)
Carried in construct	P{UAS-mid.B} (Miskolczi-McCallum et al., 2005, Buescher et al., 2004, Buescher et al., 2006, Svendsen et al., 2009, Ryu et al., 2011, Formaz-Preston et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	small body \| larval stage, with Scer\GAL4^ptc-559.1 (Buescher et al., 2004) visible \| male limited, with Scer\GAL4^bi-omb-Gal4 (Svendsen et al., 2009)
Phenotype Manifest In
	cardioblast \| ectopic, with Scer\GAL4^how-24B (Miskolczi-McCallum et al., 2005) denticle \| ectopic, with Scer\GAL4^ptc-559.1 (Buescher et al., 2004) denticle \| ectopic, with Scer\GAL4^sca-P309 (Buescher et al., 2004) denticle belt, with Scer\GAL4^prd.RG1 (Buescher et al., 2004) embryonic/larval cuticle, with Scer\GAL4^ptc-559.1 (Buescher et al., 2004) embryonic/larval cuticle, with Scer\GAL4^sca-P309 (Buescher et al., 2004) embryonic/larval dorsal vessel, with Scer\GAL4^pnr-MD237 (Miskolczi-McCallum et al., 2005) embryonic/larval dorsal vessel, with Scer\GAL4^twi.PB (Miskolczi-McCallum et al., 2005) embryonic/larval heart, with Scer\GAL4^pnr-MD237 (Miskolczi-McCallum et al., 2005) embryonic/larval heart, with Scer\GAL4^twi.PB (Miskolczi-McCallum et al., 2005) larval head, with Scer\GAL4^ptc-559.1 (Buescher et al., 2004) mesothoracic tarsal bristle, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) mesothoracic tarsal bristle longitudinal row 1 \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) mesothoracic tarsal bristle longitudinal row 8 \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) mesothoracic tibial apical bristle \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Svendsen et al., 2009) mesothoracic tibial apical bristle \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) mesothoracic tibial spur bristle \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Svendsen et al., 2009) mesothoracic tibial spur bristle \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) metathoracic tarsal bristle, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) prothoracic tarsal bristle, with Scer\GAL4^Act5C.PI (Svendsen et al., 2009) prothoracic tibial transverse row bristle \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Svendsen et al., 2009) sex comb \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Svendsen et al., 2009)
Detailed Description
	Statement Reference Scer\GAL4[bi-omb-Gal4]-driven expression of mid[Scer\UAS.cBa] results in ectopic sex combs and transverse bristle rows in the dorsal basitarsus and distal tibia of all male first legs. In the second leg, ectopic expression of Scer\GAL4[bi-omb-Gal4]-driven mid[Scer\UAS.cBa] in the dorsal tibia results in ectopic bristles similar to the apical and spur bristles. Scer\GAL4[Act5C.PI]-driven expression of mid[Scer\UAS.cBa] in small somatic clones also results in the appearance of similar ectopic bristles. Small clones of mid[Scer\UAS.cBa]-expressing cells either in or adjacent to rows 2/7 and 3/6 induce ventral row 1/8 bristles cell autonomously. Ventrolateral or dorsolateral Scer\GAL4[Act5C.PI]-driven mid[Scer\UAS.cBa]-expressing clones in the tarsus are often transformed to ventral character. (Svendsen et al., 2009) By stage 17, phenotypic effects of ectopic mid^Scer\UAS.cBa expression in the mesoderm, driven by Scer\GAL4^twi.PB, can be observed; dorsal vessel development is disrupted and in some embryos the two halves of the heart fail to fuse. The same phenotype is observed when mid^Scer\UAS.cBa is expressed in the dorsal mesoderm and ectoderm under the control of Scer\GAL4^pnr-MD237. Expression of mid^Scer\UAS.cBa throughout the mesoderm, under the control of Scer\GAL4^how-24B, causes the ectopic formation of up to 30 extra cardioblasts per half dorsal vessel, but only rarely disrupts the fusing of the two halves of the heart. (Miskolczi-McCallum et al., 2005) Expression of mid^Scer\UAS.cBa under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^sca-P309 results in nearly all naked cuticle being replaced by denticles in the posterior half of the larva. Larvae expressing mid^Scer\UAS.cBa under the control of Scer\GAL4^ptc-559.1 have a reduced body size, strong segmental indentations and head malformations. Expression of mid^Scer\UAS.cBa under the control of Scer\GAL4^prd.RG1 results in larvae with fusion of alternating denticle belts. (Buescher et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^prd.RG1, mid^Scer\UAS.cBa has denticle belt phenotype, suppressible by wg^{wg.3'UTR.Scer\UAS}/Scer\GAL4^prd.RG1 (Buescher et al., 2004)
Other
Statement Reference Mmus\Lef1^Scer\UAS.cRa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, mid^Scer\UAS.cBa has mesothoracic tarsal bristle phenotype (Svendsen et al., 2009) Mmus\Lef1^Scer\UAS.cRa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, mid^Scer\UAS.cBa has metathoracic tarsal bristle phenotype (Svendsen et al., 2009) Mmus\Lef1^Scer\UAS.cRa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, mid^Scer\UAS.cBa has prothoracic tarsal bristle phenotype (Svendsen et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of wg^{wg.3'UTR.Scer\UAS} results in near complete suppression of the fusion of alternating denticle belts seen in larvae expressing mid^Scer\UAS.cBa under the control of Scer\GAL4^prd.RG1. (Buescher et al., 2004)
Xenogenetic Interactions
Statement Reference Clones expressing both mid[Scer\UAS.cBa] and Mmus\Lef1[Scer\UAS.cRa] in the tarsus driven by Scer\GAL4[bi-omb-Gal4] are recovered more often in lateral and dorsal cells, indicating altered sorting behaviour. Dorsolateral clones expressing both mid[Scer\UAS.cBa] and Mmus\Lef1[Scer\UAS.cRa] do not transform towards ventral fate, whereas ventrolateral clones do so sometimes. (Svendsen et al., 2009)
Complementation & Rescue Data
Rescues	Scer\GAL4^sca-P309/mid^Scer\UAS.cBa rescues mid^GA174 (Buescher et al., 2004)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	mid^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Buescher (Buescher et al., 2004)
Secondary FlyBase IDs

References ( 6 )
Research paper	Formaz-Preston et al., 2012, Dev. Biol. 369(2): 319--329 The Tbx20 homolog Midline represses wingless in conjunction with Groucho during the maintenance of segment polarity. [FBrf0219199] Ryu et al., 2011, Dev. Dyn. 240(1): 86--96 Tinman is a direct activator of midline in the drosophila dorsal vessel. [FBrf0212547] Svendsen et al., 2009, Development 136(16): 2689--2693 The Tbx20 homologs midline and H15 specify ventral fate in the Drosophila melanogaster leg. [FBrf0208458] Buescher et al., 2006, Dev. Biol. 292(2): 418--429 Functions of the segment polarity genes midline and H15 in Drosophila melanogaster neurogenesis. [FBrf0190096] Miskolczi-McCallum et al., 2005, Dev. Biol. 278(2): 459--472 The Drosophila melanogaster T-box genes midline and H15 are conserved regulators of heart development. [FBrf0183853] Buescher et al., 2004, Curr. Biol. 14(19): 1694--1702 Drosophila T box proteins break the symmetry of hedgehog-dependent activation of wingless. [FBrf0180065]

Allele Dmel\midScer\UAS.cBa

Allele Dmel\mid^Scer\UAS.cBa