FlyBase Gene Report: Dmel\Orc6

General Information

Symbol

Dmel\Orc6

Species

D. melanogaster

Name

Origin recognition complex subunit 6

Annotation Symbol

CG1584

Feature Type

protein_coding_gene

FlyBase ID

FBgn0023180

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Origin recognition complex subunit 6 (Orc6) encodes a subunit of the origin recognition complex (ORC), which is essential for the initiation of DNA replication in eukaryotic cells. Orc6 product has an additional independent function in cytokinesis through the interaction with the septin protein encoded by pnut. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DmORC6, ORC

Key Links

Genomic Location

Cytogenetic map

46B4-46B4

Sequence location

2R:9,845,458..9,846,485 [-]

Recombination map

2-61

RefSeq locus

NT_033778 REGION:9845458..9846485

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables GTPase activator activity

inferred from direct assay

(Huijbregts et al., 2009)

enables protein binding

inferred from physical interaction with UniProtKB:Q9VYX1

(Kopytova et al., 2016)

enables protein homodimerization activity

inferred from direct assay

(Akhmetova et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR008721

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in DNA replication initiation

inferred from direct assay

(Chesnokov et al., 1999)

involved_in DNA replication

inferred from mutant phenotype

(Balasov et al., 2009)

involved_in mitotic cell cycle

inferred from mutant phenotype

(Balasov et al., 2009)

involved_in septin cytoskeleton organization

inferred from direct assay

(Akhmetova et al., 2015)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in DNA replication initiation

inferred from biological aspect of ancestor with PANTHER:PTN000333607

(GO Reference Genome Project, 2011-)

involved_in DNA replication

inferred from electronic annotation with InterPro:IPR008721, InterPro:IPR020529

(InterPro Project Members, 2004-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

part_of nuclear origin of replication recognition complex

inferred from direct assay

(Chesnokov et al., 1999)

located_in nucleus

inferred from direct assay

(Kopytova et al., 2016)

part_of origin recognition complex

inferred from direct assay

(Balasov et al., 2009)

colocalizes_with plasma membrane

inferred from direct assay

(Akhmetova et al., 2015)

located_in plasma membrane

inferred from direct assay

(Huijbregts et al., 2009)

located_in septin cytoskeleton

inferred from direct assay

(Akhmetova et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of nuclear origin of replication recognition complex

inferred from biological aspect of ancestor with PANTHER:PTN000333607

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR008721, InterPro:IPR020529

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

ORIGIN RECOGNITION COMPLEX

Protein Family (UniProt)

Belongs to the ORC6 family. (Q9Y1B2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ORIGIN RECOGNITION COMPLEX -: The Origin Recognition Complex (ORC) is a multi-subunit complex that binds replication origin during replication initiation. Many ORC subunits belong to the AAA+ superfamily of ATPases. (Adapted from FBrf0227879).

Protein Function (UniProtKB)

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity).

(UniProt, Q9Y1B2)

Summary (Interactive Fly)

A DNA-binding protein that functions as subunit of the Origin recognition complex - involved in initiation of DNA replication - facilitates GTPase activity and filament formation of the septin complex

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Orc6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9Y1B2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Orc6-RA

FBtr0088482

NM_057971

972

257

Additional Transcript Data and Comments

Reported size (kB)

1.2, 1.1 (northern blot)

(Chesnokov et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Orc6-PA

FBpp0087566

29.2

257

9.13

Q9Y1B2

NP_477319

AAF58890

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

257 (aa); 30 (kD observed)

(Chesnokov et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

ORC is composed of six subunits.

(UniProt, Q9Y1B2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9Y1B2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Orc6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Orc6_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.28

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium