A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Gene Dmel\Hs2st

General Information
SymbolDmel\Hs2stSpeciesD. melanogaster
Nameheparan sulfate 2-O-sulfotransferaseAnnotation symbolCG10234
Feature typeprotein_coding_geneFlyBase IDFBgn0024230
Gene Model StatusCurrent Stock availability 2 publicly available
Also Known AsDmHs2st
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map37E1-37E1Sequence location2L:19,442,830..19,444,761 [-]

Genomic Maps

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Automatically generated summary

See sections below for more information
The gene heparan sulfate 2-O-sulfotransferase is referred to in FlyBase by the symbol Dmel\Hs2st (CG10234, FBgn0024230). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: heparan sulfate 2-O-sulfotransferase activity. There is experimental evidence that it is involved in the biological process: open tracheal system development. 4 alleles are reported. The phenotypes of these alleles are annotated with: dorsal branch; presumptive embryonic/larval tracheal system. It has one annotated transcript and one annotated polypeptide. Protein features are: Heparan sulphate 2-O-sulfotransferase; Sulfotransferase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637618_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hs2st. Gene sequence location is 2L:19442830..19444761.

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Description
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What does this section not display?
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FB2011_10
FB2012_01
Sequence features
HFA03348
BKN28520
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
37E1-37E1  
Limits computationally determined from genome sequence between P{EP}dntEP2158 and P{EP}EP623&P{PZ}spi01068  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
hide Gene Model & Products
Please see the GBrowse view of Dmel\Hs2st for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
detailed view FBtr0081208 FBtr0081207 FBtr0081234 FBtr0308679 FBtr0081288 FBpp0080826 FBpp0080775 FBti0104481 FBti0059252 FBti0065466 FBti0066239 FBti0045465 FBti0044421 FBti0124865_2 FBti0066398 FBti0066399 FBti0066392 FBti0124865_1 FBti0107476 FBti0076006 FBti0007782
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Hs2st-RA
FBtr0081288
 NM_057991
  1666
  349
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Hs2st-PA  
FBpp0080826  
41.3  
349  
9.55  
  NP_477339
  AAF53800
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Heparan sulphate 2-O-sulfotransferase (IPR007734)
Sulfotransferase (IPR005331)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA441272
 
AA697515
 
AA697922
 
AE014134
AAF53800
 
AC005428
 
AC007082
 
AI388978
 
AI945462
 
AW940764
 
AY058422
AAL13651
 
AY232204
 
BF490152
 
BF491135
 
BI172716
 
BI216338
 
BI236108
 
BI237325
 
BI241241
 
CK661251
 
CO180150
 
FE426578
 
FE862688
 
FE862704
 
FE862723
 
FE862743
 
FE862993
 
FE863184
 
FE863280
 
GH740310
 
GH743734
 
GH744812
 
GH746551
 
GH761959
 
GH763230
 
GH764892
 
GH768437
 
X60218
CAA42779
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
hide Mapped Features
Mapped Features have been reorganized, please see this article for details.
Additional mapped features and mutations can be found on GBrowse or related reports.
Type
Symbol & Location
Additional Notes
References
hide External Data
Linkouts
Crossreferences
hide Expression Data
hideTranscript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4  
organism  
(Herr and Harris, 2004)
Additional Descriptive Data
Hs2st is coexpressed with CG14967
(Herr and Harris, 2004)
Marker for
Subcellular Localization
CV Term
Notes
hidePolypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
hide High-Throughput Expression Data
or
Untitled Document detailed view RanGap-RA CG10237-RD Hs2st-RA
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
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modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
hide Expression Clusters
A cluster of genes with similar mRNA expression dynamics across development.
(The modENCODE Consortium, 2010)
hide External Data & Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
•
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
•
hide Alleles & Phenotypes
hide Summary of Allele Phenotypes
Lethality
Allele
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
dorsal branch
presumptive embryonic/larval tracheal system
hide Classical Alleles ( 2 )
For All Classical Alleles Show

Allele of Hs2stClassMutagenStocksKnown lesion
Hs2st9303
0 --
Hs2std267loss of function allele
0 --
hide Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show

Allele of Hs2stClassMutagenStocksKnown lesion
Hs2stGD17722 Yes
Hs2st12A0 Yes
hide Aneuploid Aberrations
Duplicated in
(Merrill et al., 1999)
Disrupted in
(Ryder, 2004.4.26)
(Ryder, 2004.4.26)
(Ryder, 2004.4.26)
hide Transgenic Constructs & Insertions
Transgenic Constructs
Type of construct
Name
Expression data
UAS construct
P{GD1772}
NA
characterization construct
P{Sd12A}
NA
Insertions
Type of insertions
Name
Expression data
insertion of mobile activating element
P{GSV6}Hs2st9303
NA
hide Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
hide Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term
Evidence
References
heparan sulfate 2-O-sulfotransferase activity
inferred from direct assay
(Kamimura et al., 2006, Xu et al., 2007)
Biological Process
CV term
Evidence
References
open tracheal system development
inferred from genetic interaction with Hs6st
(Kamimura et al., 2006)
inferred from mutant phenotype
(Kamimura et al., 2006)
Cellular Component ( 0 terms)
hide Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term
Evidence
References
heparan sulfate 2-O-sulfotransferase activity
inferred from sequence or structural similarity with EMBL:AF060178
(FlyBase, 1992-)
Biological Process
CV term
Evidence
References
signal transduction
non-traceable author statement
(Kusano et al., 2003)
Cellular Component
CV term
Evidence
References
integral to membrane
inferred from electronic annotation with InterPro:IPR005331, InterPro:IPR007734
(FlyBase Curators et al., 2004-)
hide Sequence Ontology: Class of Gene
nuclear_gene
 
protein_coding_gene
 
hide Interactions & Pathways
hide Summary of Physical Interactions
Protein-protein
Interacting group
Assay
References
hide Summary of Genetic Interactions
Interacts with
Please look at the allele data for full details of the genetic interactions
Hs2st allele
Gene
References
hide External Data
Linkouts
DPiM - DPiM, Drosophila Protein interaction Map
•
DroID - A comprehensive database of gene and protein interactions.
•
InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.
•
hide Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
 
 
Linkouts
InParanoid A subset of ortholog calls from InParanoid.
•
OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.
•
hide Stocks & Reagents
hide Stocks Listed in FlyBase ( 2 )
VDRC
v37123
w1118; P{GD1772}v37123
v37124
w1118; P{GD1772}v37124/TM3
hide Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
hide cDNA Clones ( 31 )

Please Note
This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
hide RNAi & Array Information
Linkouts
DRSC - Results from RNAi screens.
•
GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
•
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hide Etymology
hide Identification
hide Relationship to Other Genes
Source for database identity of
Source for database merge of
Source for merge of: Hs2st CG10234
(Bayraktaroglu, 2000.4.13)
Additional comments
hide Other Comments
Heparan sulfate samples from Hs2st mutants show a loss of 2-O sulfated disaccharide units, as well as an increase in 6-O sulfated disaccharides. The level of total sulfate groups on heparan sulfate is not affected, and the total charge of heparan sulfate is almost wild-type.
(Kamimura et al., 2006)
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
(Kiger et al., 2003)
hide External Crossreferences & Linkouts
Sequence Crossreferences
RefSeq (Transcripts)
•
RefSeq (Proteins)
•
DDBJ / EMBL / GenBank
•
Entrez Gene - A searchable database of RefSeq genes.
•
UniProtKB/Swiss-Prot
•
Other Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Heparan sulphate 2-O-sulfotransferase (IPR007734)
Sulfotransferase (IPR005331)
Linkouts
DPiM - DPiM, Drosophila Protein interaction Map
•
DroID - A comprehensive database of gene and protein interactions.
•
DRSC - Results from RNAi screens.
•
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
•
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
•
FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
•
GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
•
InParanoid A subset of ortholog calls from InParanoid.
•
InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.
•
modMine - Data generated by the modENCODE project.
•
OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.
•
hide Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym
CG10234
 
dHS2ST
(Merrill et al., 1999)
dmHS-2OST
(Xu et al., 2007)
DmHs2st
(Nybakken and Perrimon, 2002)
dmHs2st
(Xu et al., 2007)
HS2ST
(Kusano et al., 2003, McLean et al., 1999)
Hs2st
(Kamimura et al., 2006, Presgraves et al., 2009)
Hs2st-2
(Presgraves et al., 2009)
Name Synonym
heparan sulfate 2-O-sulfotransferase
(Xu et al., 2007)
heparan-sulfate-2-sulfotransferase
 
HS 2-O
(Kamimura et al., 2006)
Hs2st
 
Secondary FlyBase IDs
  • FBgn0032784
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