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Gene Dmel\Hs2st

General Information
Symbol	Dmel\Hs2st	Species	D. melanogaster
Name	Heparan sulfate 2-O-sulfotransferase	Annotation symbol	CG10234
Feature type	protein_coding_gene	FlyBase ID	FBgn0024230
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	DmHs2st
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	37E1-37E1	Sequence location	2L:19,442,833..19,444,761 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heparan sulfate 2-O-sulfotransferase is referred to in FlyBase by the symbol Dmel\Hs2st (CG10234, FBgn0024230). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: heparan sulfate 2-O-sulfotransferase activity. There is experimental evidence that it is involved in the biological process: open tracheal system development. 5 alleles are reported. The phenotypes of these alleles are annotated with: dorsal branch; presumptive embryonic/larval tracheal system. It has one annotated transcript and one annotated polypeptide. Protein features are: Heparan sulphate 2-O-sulfotransferase; Sulfotransferase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637618_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hs2st. Gene sequence location is 2L:19442833..19444761.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 37E1-37E1   Limits computationally determined from genome sequence between P{EP}dntEP2158 and P{EP}EP623&P{PZ}spi01068
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hs2st for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hs2st-RA FBtr0081288  NM_057991   1663   349
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hs2st-PA   FBpp0080826   41.3   349   9.55     NP_477339   AAF53800
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Heparan sulphate 2-O-sulfotransferase (IPR007734) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Sulfotransferase (IPR005331)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA441272   AA697515   AA697922   AE014134 AAF53800   AC005428   AC007082   AI388978   AI945462   AW940764   AY058422 AAL13651   AY232204   BF490152   BF491135   BI172716   BI216338   BI236108   BI237325   BI241241   CK661251   CO180150   FE426578   FE862688   FE862704   FE862723   FE862743   FE862993   FE863184   FE863280   GH740310   GH743734   GH744812   GH746551   GH761959   GH763230   GH764892   GH768437   X60218 CAA42779
UniProtKB/Swiss-Prot	P25722
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4   organism   (Herr and Harris, 2004) embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
	Hs2st is coexpressed with CG14967 (Herr and Harris, 2004)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    79.175 Larval Midgut    69.4 Larval Hindgut    86.6 Larval Malpighian Tubules    166.5 Larval Fat Body    294.6 Larval Salivary Gland    198.1 Larval Trachea    165.75 Larval Carcass    129.175 Adult Head    97.1 Adult Eye    78.8 Adult Brain    89.6 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    123.7 Adult Midgut    42 Adult Hindgut    71.5 Adult Malpighian Tubules    52.5 Adult Fat Body    212.6 Adult Salivary Gland    120 Adult Heart    165.75 Adult VirginFemale Spermatheca    151.2 Adult InseminatedFemale Spermatheca    151.1 Adult Ovary    253.2 Adult Testis    65.5 Adult Male Accessory Gland    55 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    97.1   NA  Eye    78.8   NA  Brain    89.6   79.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    63.8   NA  Crop    123.7   69.4  Midgut    42   86.6  Hindgut    71.5   166.5  Malpighian Tubules    52.5   294.6  Fat Body    212.6   198.1  Salivary Gland    120   NA  Heart    165.75   165.75  Trachea    NA   NA  VirginFemale Spermatheca    151.2   NA  InseminatedFemale Spermatheca    151.1   NA  Ovary    253.2   NA  Testis    65.5   NA  Male Accessory Gland    55   129.175  Carcass    102.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female    44 ovary, mated 4-day female    33 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (33) testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female    44 ovary, mated 4-day female    33 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female    44 ovary, mated 4-day female    33 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female    44 ovary, mated 4-day female    33 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (33) testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female    44 ovary, mated 4-day female    33 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    11 central nervous system, pupae P8    15 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    13 head, mated 1-day female    7 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    12 head, mated 4-day male    14 head, mated 20-day male    16 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    12 fat body, white prepupae    18 fat body, pupae P8    13 carcass, larvae L3 wandering    18 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    13 ovary, virgin 4-day female    44 ovary, mated 4-day female    33 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    9 embryo 04-06hr    13 embryo 06-08hr    12 embryo 08-10hr    11 embryo 10-12hr    9 embryo 12-14hr    9 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    5 larva L2    7 larva L3 12hr old    8 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    20 white prepupae new    18 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    14 pupae 3d postWPP    9 pupae 4d postWPP    6 adult male 01day    8 adult male 05day    9 adult male 30day    7 adult female 01day    10 adult female 05day    21 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1    29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1  (29) embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1    29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1    29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1    29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1  29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1    29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    17 embryonic 1182-4H    13 embryonic GM2    24 embryonic Kc167    21 embryonic S1    29 embryonic S3    12 leg disc CME L1    19 wing disc CME-W2    22 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    5 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • GH20044 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024230 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10234-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die, with Scer\GAL4Act.PU Hs2stScer\UAS.cKa viable, with Scer\GAL4hh.PU Hs2stScer\UAS.cKa viable, with Scer\GAL4pnr-MD237 Hs2stGD1772 Phenotype manifest in Allele dorsal branch Hs2std267 presumptive embryonic/larval tracheal system Hs2std267
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Hs2st Class Mutagen Stocks Known lesion Hs2st9303 0 -- Hs2std267 loss of function allele 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hs2st Class Mutagen Stocks Known lesion Hs2stGD1772 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Hs2st12A in vitro construct 0 Yes Hs2stScer\UAS.cKa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Duplicated in	Dp(2;2)RanGapSD (Merrill et al., 1999)
Disrupted in	Df(2L)Exel7075 (Ryder, 2004.4.26) Df(2L)Exel7076 (Ryder, 2004.4.26) Df(2L)Exel8041 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1772} NA P{UAS-Hs2st.K} NA characterization construct P{Sd12A} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{GSV6}Hs2st9303 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term Evidence References
heparan sulfate 2-O-sulfotransferase activity inferred from direct assay (Kamimura et al., 2006, Xu et al., 2007)
Biological Process
CV term Evidence References
open tracheal system development inferred from genetic interaction with Hs6st (Kamimura et al., 2006) inferred from mutant phenotype (Kamimura et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
heparan sulfate 2-O-sulfotransferase activity inferred from sequence or structural similarity with EMBL:AF060178 (FlyBase, 1992-)
Biological Process
CV term Evidence References
signal transduction non-traceable author statement (Kusano et al., 2003)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR005331, InterPro:IPR007734 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hs2st - CG15098 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - CG43444 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - CG6506 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Hs2st - CG7049 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - Cg25C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - tmod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hs2st - vkg anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hs2st allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024230 DroID - A comprehensive database of gene and protein interactions. • FBgn0024230 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44433
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG608NTZ Dipteran inclusive ortholog search EOG6KKZBN Insect inclusive ortholog search EOG64MWNT Arthropod inclusive ortholog search EOG6905RR Metazoa inclusive ortholog search EOG62ZHHW
Orthologs in Drosophila Species (EOG608NTZ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hs2st     Drosophila simulans   Dsim\GD21759 Y   Drosophila sechellia   Dsec\GM17009 Y   Drosophila erecta   Dere\GG21631 Y   Drosophila yakuba   Dyak\Hs2st Y   Drosophila ananassae   Dana\GF15842 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10178 Y   Drosophila persimilis   Dper\GL21106 Y   Drosophila willistoni   Dwil\GK18194 Y   Drosophila virilis   Dvir\GJ17976 Y   Drosophila mojavensis   Dmoj\GI17219 Y   Drosophila grimshawi   Dgri\GH13467 Y
Orthologs in non-Drosophila Dipterans (EOG6KKZBN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003346   Anopheles gambiae Malaria mosquito  Agam\AGAP008166   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801420
Orthologs in non-Dipteran Insects (EOG64MWNT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB47193   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005350   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12870   Atta cephalotes Leafcutter ant  Acep\ACEP21971   Camponotus floridanus Florida carpenter ant  Cflo\CFLO23266   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23074   Linepithema humile Argentine ant  Lhum\LH11989   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16407   Solenopsis invicta Red fire ant  Sinv\SINV19957   Acyrthosiphon pisum Pea aphid  Apim\ACYPI069516   Bombyx mori Silkmoth  Bmor\BGIBMGA006029   Pediculus humanus Human body louse  Phum\PHUM363280   Tribolium castaneum Red flour beetle  Tcas\Hs2st
Orthologs in non-Insect Arthropods (EOG6905RR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\coi   Ixodes scapularis Deer tick  Isca\ISCW000208
Orthologs in non-Arthropod Metazoa (EOG62ZHHW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\hst-2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004260gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005843gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009508gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007334gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024278gn   Danio rerio Zebrafish  Drer\ENSDARG00000062008   Danio rerio Zebrafish  Drer\hs2st1a   Xenopus tropicalis Western clawed frog  Xtro\hs2st1   Gallus gallus Domestic chicken  Ggal\HS2ST1   Mus musculus House mouse  Mmus\Hs2st1   Rattus norvegicus Norway rat  Rnor\Hs2st1   Homo sapiens Human  Hsap\ENSG00000153936
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000153936
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21759 Drosophila sechellia Dsec\GM17009 Drosophila erecta Dere\GG21631 Drosophila yakuba Dyak\Hs2st Drosophila ananassae Dana\GF15842 Drosophila pseudoobscura pseudoobscura Dpse\GA10178 Drosophila persimilis Dper\GL21106 Drosophila willistoni Dwil\GK18194 Drosophila virilis Dvir\GJ17976 Drosophila mojavensis Dmoj\GI17219 Drosophila grimshawi Dgri\GH13467
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v37123 w1118; P{GD1772}v37123 v37124 w1118; P{GD1772}v37124/TM3
Genomic Clones ( 2 )
	BACR27M12 BACR28G24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 31 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH20044
Other clones	DPiM_GH20044
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT26450 AT27807 HL02702 HL03464 LD16148 LP20582 RE15375 RE23442 RE32111 RE33538 RE38266
Other clones	bs13b09 EC01056 RM05686 RM05886 RM05986 RM06086 RM06186 RM06286 RM06386 RM06586 RP001011475 RP001014684 RP001020677 RP001021893 RP001032456 RP001032551 RP001034202 RP001034785
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024230 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44433
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Hs2st CG10234 (Bayraktaroglu, 2000.4.13)
Additional comments
Other Comments
	Heparan sulfate samples from Hs2st mutants show a loss of 2-O sulfated disaccharide units, as well as an increase in 6-O sulfated disaccharides. The level of total sulfate groups on heparan sulfate is not affected, and the total charge of heparan sulfate is almost wild-type. (Kamimura et al., 2006) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057991 RefSeq (Proteins) • NP_477339 DDBJ / EMBL / GenBank • AAF53800 AAL13651 AC005428 AC007082 AI388978 AI945462 AW940764 AY058422 CAA42779 X60218 Entrez Gene - A searchable database of RefSeq genes. • 44433 UniProtKB/Swiss-Prot • P25722
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Heparan sulphate 2-O-sulfotransferase (IPR007734) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Sulfotransferase (IPR005331) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH20044
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024230 DroID - A comprehensive database of gene and protein interactions. • FBgn0024230 DRSC - Results from RNAi screens. • FBgn0024230 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024230 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10234-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024230 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44433 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44433 modMine - Data generated by the modENCODE project. • FBgn0024230
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG10234   dHS2ST (Merrill et al., 1999) dmHS-2OST (Xu et al., 2007) DmHs2st (Nybakken and Perrimon, 2002) dmHs2st (Xu et al., 2007) HS2ST (Kusano et al., 2003, McLean et al., 1999) Hs2st (Kamimura et al., 2006, Kamimura et al., 2011, Presgraves et al., 2009, Hayashi et al., 2012) Hs2st-2 (Presgraves et al., 2009)
Name Synonym	2-O sulfotransferase (Kamimura et al., 2011) heparan sulfate 2-O-sulfotransferase (Xu et al., 2007) Heparan sulfate 2-O-sulfotransferase   heparan-sulfate-2-sulfotransferase   HS 2-O (Kamimura et al., 2006) Hs2st
Secondary FlyBase IDs
	FBgn0032784
References ( 31 )
Generate a list of	All references relating to this gene ( 31 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 4 ) Review  ( 3 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Dejima et al., 2013, J. Biol. Chem. 288(9): 6574--6582 The role of Drosophila heparan sulfate 6-o-endosulfatase in sulfation compensation. [FBrf0220932] Hayashi et al., 2012, Development 139(22): 4162--4171 Glypicans regulate JAK/STAT signaling and distribution of the Unpaired morphogen. [FBrf0219707] Kamimura et al., 2011, Glycobiology 21(5): 607--618 In vivo manipulation of heparan sulfate structure and its effect on Drosophila development. [FBrf0213406] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...