Reference Report

Reference
Citation	Long, A.A., Kim, E., Leung, H.T., Woodruff, E., An, L., Doerge, R.W., Pak, W.L., Broadie, K. (2008). Presynaptic calcium channel localization and calcium-dependent synaptic vesicle exocytosis regulated by the Fuseless protein. J. Neurosci. 28(14): 3668--3682. (Export to RIS)
FlyBase ID	FBrf0204409
Publication Type	Research paper
PubMed ID	18385325
PubMed Abstract	A systematic forward genetic Drosophila screen for electroretinogram mutants lacking synaptic transients identified the fuseless (fusl) gene, which encodes a predicted eight-pass transmembrane protein in the presynaptic membrane. Null fusl mutants display >75% reduction in evoked synaptic transmission but, conversely, an approximately threefold increase in the frequency and amplitude of spontaneous synaptic vesicle fusion events. These neurotransmission defects are rescued by a wild-type fusl transgene targeted only to the presynaptic cell, demonstrating a strictly presynaptic requirement for Fusl function. Defects in FM dye turnover at the synapse show a severely impaired exo-endo synaptic vesicle cycling pool. Consistently, ultrastructural analyses reveal accumulated vesicles arrested in clustered and docked pools at presynaptic active zones. In the absence of Fusl, calcium-dependent neurotransmitter release is dramatically compromised and there is little enhancement of synaptic efficacy with elevated external Ca(2+) concentrations. These defects are causally linked with severe loss of the Cacophony voltage-gated Ca(2+) channels, which fail to localize normally at presynaptic active zone domains in the absence of Fusl. These data indicate that Fusl regulates assembly of the presynaptic active zone Ca(2+) channel domains required for efficient coupling of the Ca(2+) influx and synaptic vesicle exocytosis during neurotransmission.
DOI	10.1523/JNEUROSCI.5553-07.2008
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (2)
	Df(2L)cl7 Df(2L)Exel6012
Alleles (9)
	cac^{Scer\UAS.T:Avic\GFP-EGFP} fusl¹ fusl² fusl^{RA.T:Avic\GFP-EGFP} fusl^{RB.T:Avic\GFP-EGFP} fusl^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^elav.PLu T:Avic\GFP^EGFP w^+mC
Constructs (5)
	P{fusl^{RA.T:Avic\GFP-EGFP}} P{fusl^{RB.T:Avic\GFP-EGFP}} P{GAL4-elav.L} P{UAS-cac1-EGFP} P{UAS-fusl.eGFP}
Genes (7)
	cac cype fusl Scer\GAL4 Syt1 vri w
Natural transposons (1)
	P-element