[98A14-98A14];[98B5-98B5];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
98A14;98B5
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Four-week old Df(3R)BSC499/Df(3R)MR22 mutant flies exhibit retinal degeneration. The retinas and rhabdomeres are indistinguishable from wild type at 1 day after eclosion, but show a loosening of the microvilli at 7 days. No photoreceptor loss is seen. An increased number of vacuoles is seen in the brains of 28-day old flies compared to age-matched controls. The axons of 1-day old flies are similar to controls but occasionally exhibit abnormal gaps between the axons. By 16 days, many more and larger extracellular gaps are seen between the axons. There is an increase in the cross sectional area of microtubules in 1-day old Df(3R)BSC499/Df(3R)MR22 mutant flies compared to controls, as well as a reduction in the density of microtubules per neurite.
Inferred to overlap with: Df(3R)BSC42.
The presence of P+PBac{XP5.WH5}BSC499 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(3R)BSC499 predicted from the Release 5 genomic coordinates of the progenitor PBac{WH}tauf00369 and P{XP}d05341 insertion sites are 98A14;98B5.