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General Information
Symbol
Dmel\Sumo04493
Species
D. melanogaster
Name
FlyBase ID
FBal0008063
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)04493, smt304493, smt34493, P{PZ}smt304493
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion about 10bp upstream of the first exon.

P{PZ} insertion 33bp upstream of the 5' end of the CT14617 transcript of smt3.

Insertion components
P{PZ}Sumo04493
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Less than 30% of smt304493 germ-line clone (GLC) embryos hatch and all hatched larvae die during the first larval instar. Greater than 90% of the unhatched embryos die prior to cuticle formation. The embryos that deposit cuticle exhibit a wide range of both anteroposterior and dorsoventral patterning defects. These defects are not rescued by zygotic smt3 expression as embryos produced by GLC females mated with wild type males show a comparable spectrum of phenotypes as those embryos produced by GLC females mated with heterozygous males.

Approximately 10% of smt304493 GLC eggs exhibit partially to fully fused dorsal appendages.

Over 50% of smt304493 GLC embryos exhibit a broad spectrum of nuclear cycle defects, including irregular size and distribution of nuclei, asynchronous nuclear division, abnormal interphase chromosome structure, overly condensed chromosomes, loss of sister chromatid cohesion during metaphase, polyploidy, chromosome clustering, fragmentation, and chromosome bridges. smt304493 GLC embryos often contain a reduced number of nuclei in relation to the centrosome pairs, and show defects in mitotic spindle organization and attachment to centrosomes and chromosomes.

Average Citrate Synthase activity is increased by 6% in heterozygous males

0.76% of smt304493/smt3k01211 animals survive as adult escapers. These females produce few late-stage egg chambers and these rare eggs have poorly developed dorsal appendages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

Sumo04493/smt3[+] is an enhancer of visible | dominant phenotype of tna1

Sumo04493/smt3[+] is an enhancer of visible | dominant phenotype of osa1

Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhancer of
Statement
Reference

Sumo04493/smt3[+] is an enhancer of wing phenotype of tna1

Sumo04493/smt3[+] is an enhancer of wing phenotype of osa1

Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

smt304493/+ enhances both the penetrance and expressivity of the held-out wing phenotype of tna1/+ flies. A maternal effect is observed in the enhancement of the tna1/+ held out phenotype by smt304493/+.

smt304493/+ augments the penetrance and the expressivity of the weak held-out wing phenotype of osa1/+.

A smt304493/+ background suppresses the visual system degeneration seen in Scer\GAL4GMR.PF, Ppt1Scer\UAS.cKa flies.

smt304493/Df(2L)32FP-5 double heterozygotes have ectopic wing vein L3 material in the L2-L3 intervein region in 86% of cases. The wing size is reduced in size by 10% compared to wild type.

The transheterozygote lwr05486/smt304493 suppresses the mod(mdg4)ul suppression of the ct6 jagged wing phenotype, leading to a more jagged and discontinuous wing phenotype. The phenotype is also particularly pronounced in females, although it is detectable in both sexes.

The transheterozygote lwr05486/smt304493 suppresses the mod(mdg4)ul suppression of the y2 cuticle phenotype, leading to a lighter coloration of the abdomen (due to a reduction in y expression).These phenotypic changes occur in approximately 30% of the mutant flies, with the remaining 70% displaying a continuum of less-pronounced effect. The phenotype is also particularly pronounced in females, although it is detectable in both sexes.

Enhancer of the Ras85Dix12a eggshell phenotype; 57% of eggs have fused and forked dorsal appendages.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements: snRNP70K02107. Complements: Hrb27C02647. Complements: l(2)k00213k00213. Complements: miltk04704. Complements: Coproxk10617. Complements: Hrb27CrF680. Complements: poe03420. Complements: cuc06491.

Embryos derived from smt3k01211 and smt304493 germ-line clones (GLC) show an overlapping spectra of defects, with the average severity of defects being greater in smt3k01211 GLC embryos than smt304493 GLC embryos.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (17)